Anti-Aging Effect and Mechanism of Proanthocyanidins Extracted from <i>Sea buckthorn</i> on Hydrogen Peroxide-Induced Aging Human Skin Fibroblasts
Oxidative stress is the leading cause of skin aging damage. Excessive accumulation of reactive oxygen species (ROS) in cells induced by hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) triggers a decrease in collagen synthesis and an increase in collagen degradation, which are...
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MDPI AG
2022-09-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/10/1900 |
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| author | Xinying Liu Yi Xing Michael Yuen Tina Yuen Hywel Yuen Qiang Peng |
| author_facet | Xinying Liu Yi Xing Michael Yuen Tina Yuen Hywel Yuen Qiang Peng |
| author_sort | Xinying Liu |
| collection | DOAJ |
| description | Oxidative stress is the leading cause of skin aging damage. Excessive accumulation of reactive oxygen species (ROS) in cells induced by hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) triggers a decrease in collagen synthesis and an increase in collagen degradation, which are biomarkers of skin aging. We evaluated the potential protective mechanism of <i>Sea buckthorn</i> proanthocyanidins (SBP) against the oxidative stress-induced skin aging process from multiple aspects. We treated human skin fibroblasts (HSFs) with 300 µmoL/L of H<sub>2</sub>O<sub>2</sub> for 24 h, followed by 25, 50, and 100 µg/mL of SBP for 24 h. The results showed that SBP could enhance the activities of superoxide dismutase (SOD) and glutathione (GSH), effectively remove excess ROS, and significantly improve the changes in cell morphology and viability caused by excessive ROS in skin cells. In addition, SBP could promote the synthesis of Col I in aging HSFs through the TGF-β1/Smads pathway and inhibit the degradation of Col I by regulating the MMPs/TIMPs system, thereby maintaining the stability of the ECM structure to achieve anti-aging purposes. Finally, we studied the migration ability of SBP, and the results showed that 100 µg/mL of SBP was most conducive to the cell migration of senescent cells, laying a foundation for follow-up animal experiments. These results will increase the application value of SBP in the cosmetic and antioxidative functional food industries. |
| first_indexed | 2024-03-09T20:49:59Z |
| format | Article |
| id | doaj.art-dea36a1b9cbf4874859b1c031a5e5270 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T20:49:59Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-dea36a1b9cbf4874859b1c031a5e52702023-11-23T22:37:34ZengMDPI AGAntioxidants2076-39212022-09-011110190010.3390/antiox11101900Anti-Aging Effect and Mechanism of Proanthocyanidins Extracted from <i>Sea buckthorn</i> on Hydrogen Peroxide-Induced Aging Human Skin FibroblastsXinying Liu0Yi Xing1Michael Yuen2Tina Yuen3Hywel Yuen4Qiang Peng5College of Food Science and Engineering, Northwest A&F University, Yangling, Xianyang 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling, Xianyang 712100, ChinaPuredia Limited, Xining 810000, ChinaPuredia Limited, Xining 810000, ChinaPuredia Limited, Xining 810000, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling, Xianyang 712100, ChinaOxidative stress is the leading cause of skin aging damage. Excessive accumulation of reactive oxygen species (ROS) in cells induced by hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) triggers a decrease in collagen synthesis and an increase in collagen degradation, which are biomarkers of skin aging. We evaluated the potential protective mechanism of <i>Sea buckthorn</i> proanthocyanidins (SBP) against the oxidative stress-induced skin aging process from multiple aspects. We treated human skin fibroblasts (HSFs) with 300 µmoL/L of H<sub>2</sub>O<sub>2</sub> for 24 h, followed by 25, 50, and 100 µg/mL of SBP for 24 h. The results showed that SBP could enhance the activities of superoxide dismutase (SOD) and glutathione (GSH), effectively remove excess ROS, and significantly improve the changes in cell morphology and viability caused by excessive ROS in skin cells. In addition, SBP could promote the synthesis of Col I in aging HSFs through the TGF-β1/Smads pathway and inhibit the degradation of Col I by regulating the MMPs/TIMPs system, thereby maintaining the stability of the ECM structure to achieve anti-aging purposes. Finally, we studied the migration ability of SBP, and the results showed that 100 µg/mL of SBP was most conducive to the cell migration of senescent cells, laying a foundation for follow-up animal experiments. These results will increase the application value of SBP in the cosmetic and antioxidative functional food industries.https://www.mdpi.com/2076-3921/11/10/1900<i>Sea buckthorn</i> proanthocyanidinsanti-agingtype I collagenoxidative stressreactive oxygen species |
| spellingShingle | Xinying Liu Yi Xing Michael Yuen Tina Yuen Hywel Yuen Qiang Peng Anti-Aging Effect and Mechanism of Proanthocyanidins Extracted from <i>Sea buckthorn</i> on Hydrogen Peroxide-Induced Aging Human Skin Fibroblasts Antioxidants <i>Sea buckthorn</i> proanthocyanidins anti-aging type I collagen oxidative stress reactive oxygen species |
| title | Anti-Aging Effect and Mechanism of Proanthocyanidins Extracted from <i>Sea buckthorn</i> on Hydrogen Peroxide-Induced Aging Human Skin Fibroblasts |
| title_full | Anti-Aging Effect and Mechanism of Proanthocyanidins Extracted from <i>Sea buckthorn</i> on Hydrogen Peroxide-Induced Aging Human Skin Fibroblasts |
| title_fullStr | Anti-Aging Effect and Mechanism of Proanthocyanidins Extracted from <i>Sea buckthorn</i> on Hydrogen Peroxide-Induced Aging Human Skin Fibroblasts |
| title_full_unstemmed | Anti-Aging Effect and Mechanism of Proanthocyanidins Extracted from <i>Sea buckthorn</i> on Hydrogen Peroxide-Induced Aging Human Skin Fibroblasts |
| title_short | Anti-Aging Effect and Mechanism of Proanthocyanidins Extracted from <i>Sea buckthorn</i> on Hydrogen Peroxide-Induced Aging Human Skin Fibroblasts |
| title_sort | anti aging effect and mechanism of proanthocyanidins extracted from i sea buckthorn i on hydrogen peroxide induced aging human skin fibroblasts |
| topic | <i>Sea buckthorn</i> proanthocyanidins anti-aging type I collagen oxidative stress reactive oxygen species |
| url | https://www.mdpi.com/2076-3921/11/10/1900 |
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