Grapevine <i>DMR6-1</i> Is a Candidate Gene for Susceptibility to Downy Mildew
Grapevine (<i>Vitis vinifera</i>) is a valuable crop in Europe for both economical and cultural reasons, but highly susceptible to Downy mildew (DM). The generation of resistant vines is of critical importance for a sustainable viticulture and can be achieved either by introgression of r...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-01-01
|
| Series: | Biomolecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-273X/12/2/182 |
| _version_ | 1797482303202525184 |
|---|---|
| author | Carlotta Pirrello Giulia Malacarne Marco Moretto Luisa Lenzi Michele Perazzolli Tieme Zeilmaker Guido Van den Ackerveken Stefania Pilati Claudio Moser Lisa Giacomelli |
| author_facet | Carlotta Pirrello Giulia Malacarne Marco Moretto Luisa Lenzi Michele Perazzolli Tieme Zeilmaker Guido Van den Ackerveken Stefania Pilati Claudio Moser Lisa Giacomelli |
| author_sort | Carlotta Pirrello |
| collection | DOAJ |
| description | Grapevine (<i>Vitis vinifera</i>) is a valuable crop in Europe for both economical and cultural reasons, but highly susceptible to Downy mildew (DM). The generation of resistant vines is of critical importance for a sustainable viticulture and can be achieved either by introgression of resistance genes in susceptible varieties or by mutation of Susceptibility (S) genes, e.g., by gene editing. This second approach offers several advantages: it maintains the genetic identity of cultivars otherwise disrupted by crossing and generally results in a broad-spectrum and durable resistance, but it is hindered by the poor knowledge about S genes in grapevines. Candidate S genes are <i>Downy mildew Resistance 6</i> (<i>DMR6</i>) and <i>DMR6-Like Oxygenases</i> (<i>DLO</i>s), whose mutations confer resistance to DM in Arabidopsis. In this work, we show that grapevine <i>VviDMR6-1</i> complements the Arabidopsis <i>dmr6-1</i> resistant mutant. We studied the expression of grapevine <i>VviDMR6</i> and <i>VviDLO</i> genes in different organs and in response to the DM causative agent <i>Plasmopara viticola</i>. Through an automated evaluation of causal relationships among genes, we show that <i>VviDMR6-1</i>, <i>VviDMR6-2</i>, and <i>VviDLO1</i> group into different co-regulatory networks, suggesting distinct functions, and that mostly <i>VviDMR6-1</i> is connected with pathogenesis-responsive genes. Therefore, <i>VviDMR6-1</i> represents a good candidate to produce resistant cultivars with a gene-editing approach. |
| first_indexed | 2024-03-09T22:31:20Z |
| format | Article |
| id | doaj.art-dea634f1ee75430fbc1fe2f4ed06397f |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-09T22:31:20Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-dea634f1ee75430fbc1fe2f4ed06397f2023-11-23T18:57:47ZengMDPI AGBiomolecules2218-273X2022-01-0112218210.3390/biom12020182Grapevine <i>DMR6-1</i> Is a Candidate Gene for Susceptibility to Downy MildewCarlotta Pirrello0Giulia Malacarne1Marco Moretto2Luisa Lenzi3Michele Perazzolli4Tieme Zeilmaker5Guido Van den Ackerveken6Stefania Pilati7Claudio Moser8Lisa Giacomelli9Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, 38098 San Michele all’Adige, ItalyResearch and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, 38098 San Michele all’Adige, ItalyResearch and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, 38098 San Michele all’Adige, ItalyResearch and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, 38098 San Michele all’Adige, ItalyResearch and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, 38098 San Michele all’Adige, ItalySciENZA Biotechnologies B.V., Sciencepark 904, 1098 XH Amsterdam, The NetherlandsPlant-Microbe Interactions, Department of Biology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The NetherlandsResearch and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, 38098 San Michele all’Adige, ItalyResearch and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, 38098 San Michele all’Adige, ItalyResearch and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, 38098 San Michele all’Adige, ItalyGrapevine (<i>Vitis vinifera</i>) is a valuable crop in Europe for both economical and cultural reasons, but highly susceptible to Downy mildew (DM). The generation of resistant vines is of critical importance for a sustainable viticulture and can be achieved either by introgression of resistance genes in susceptible varieties or by mutation of Susceptibility (S) genes, e.g., by gene editing. This second approach offers several advantages: it maintains the genetic identity of cultivars otherwise disrupted by crossing and generally results in a broad-spectrum and durable resistance, but it is hindered by the poor knowledge about S genes in grapevines. Candidate S genes are <i>Downy mildew Resistance 6</i> (<i>DMR6</i>) and <i>DMR6-Like Oxygenases</i> (<i>DLO</i>s), whose mutations confer resistance to DM in Arabidopsis. In this work, we show that grapevine <i>VviDMR6-1</i> complements the Arabidopsis <i>dmr6-1</i> resistant mutant. We studied the expression of grapevine <i>VviDMR6</i> and <i>VviDLO</i> genes in different organs and in response to the DM causative agent <i>Plasmopara viticola</i>. Through an automated evaluation of causal relationships among genes, we show that <i>VviDMR6-1</i>, <i>VviDMR6-2</i>, and <i>VviDLO1</i> group into different co-regulatory networks, suggesting distinct functions, and that mostly <i>VviDMR6-1</i> is connected with pathogenesis-responsive genes. Therefore, <i>VviDMR6-1</i> represents a good candidate to produce resistant cultivars with a gene-editing approach.https://www.mdpi.com/2218-273X/12/2/182<i>DMR6</i><i>DLO</i>downy mildew<i>Plasmopara viticola</i><i>Vitis vinifera</i> |
| spellingShingle | Carlotta Pirrello Giulia Malacarne Marco Moretto Luisa Lenzi Michele Perazzolli Tieme Zeilmaker Guido Van den Ackerveken Stefania Pilati Claudio Moser Lisa Giacomelli Grapevine <i>DMR6-1</i> Is a Candidate Gene for Susceptibility to Downy Mildew Biomolecules <i>DMR6</i> <i>DLO</i> downy mildew <i>Plasmopara viticola</i> <i>Vitis vinifera</i> |
| title | Grapevine <i>DMR6-1</i> Is a Candidate Gene for Susceptibility to Downy Mildew |
| title_full | Grapevine <i>DMR6-1</i> Is a Candidate Gene for Susceptibility to Downy Mildew |
| title_fullStr | Grapevine <i>DMR6-1</i> Is a Candidate Gene for Susceptibility to Downy Mildew |
| title_full_unstemmed | Grapevine <i>DMR6-1</i> Is a Candidate Gene for Susceptibility to Downy Mildew |
| title_short | Grapevine <i>DMR6-1</i> Is a Candidate Gene for Susceptibility to Downy Mildew |
| title_sort | grapevine i dmr6 1 i is a candidate gene for susceptibility to downy mildew |
| topic | <i>DMR6</i> <i>DLO</i> downy mildew <i>Plasmopara viticola</i> <i>Vitis vinifera</i> |
| url | https://www.mdpi.com/2218-273X/12/2/182 |
| work_keys_str_mv | AT carlottapirrello grapevineidmr61iisacandidategeneforsusceptibilitytodownymildew AT giuliamalacarne grapevineidmr61iisacandidategeneforsusceptibilitytodownymildew AT marcomoretto grapevineidmr61iisacandidategeneforsusceptibilitytodownymildew AT luisalenzi grapevineidmr61iisacandidategeneforsusceptibilitytodownymildew AT micheleperazzolli grapevineidmr61iisacandidategeneforsusceptibilitytodownymildew AT tiemezeilmaker grapevineidmr61iisacandidategeneforsusceptibilitytodownymildew AT guidovandenackerveken grapevineidmr61iisacandidategeneforsusceptibilitytodownymildew AT stefaniapilati grapevineidmr61iisacandidategeneforsusceptibilitytodownymildew AT claudiomoser grapevineidmr61iisacandidategeneforsusceptibilitytodownymildew AT lisagiacomelli grapevineidmr61iisacandidategeneforsusceptibilitytodownymildew |