Expression Pattern of iNOS, BCL-2 and MMP-9 in the Hip Synovium Tissue of Patients with Osteoarthritis
Hip osteoarthritis (HOA) is characterized by degradation of the cartilage and synovitis. However, the pathohistological effects of synovial tissue inflammation on HOA are not clear. The aim of this study was to evaluate the expression of iNOS, BCL-2 and MMP-9 markers in different synovial cell popul...
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MDPI AG
2021-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/3/1489 |
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| author | Davor Caric Sandra Zekic Tomas Natalija Filipovic Violeta Soljic Benjamin Benzon Sandro Glumac Ivan Rakovac Katarina Vukojevic |
| author_facet | Davor Caric Sandra Zekic Tomas Natalija Filipovic Violeta Soljic Benjamin Benzon Sandro Glumac Ivan Rakovac Katarina Vukojevic |
| author_sort | Davor Caric |
| collection | DOAJ |
| description | Hip osteoarthritis (HOA) is characterized by degradation of the cartilage and synovitis. However, the pathohistological effects of synovial tissue inflammation on HOA are not clear. The aim of this study was to evaluate the expression of iNOS, BCL-2 and MMP-9 markers in different synovial cell populations. A total of 32 patients were evaluated retrospectively. Age, sex, height, weight, body mass index were recorded and lymphocyte, fibrocytes and macrophages were analysed in tissue sections. Osteoarthritis cartilage histopathology assessment system (OARSI), Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), Krenn score, Harris Hip Score (HHS) and Kellgren–Lawrence (K-L) grading of the hip joints were performed. Total hip arthroplasty was performed on 32 patients and controls. Patients were divided into two groups according to their disease severity. The tissues were immunohistochemically analysed. K-L grade and Krenn score differ between all three groups, but also between moderate and severe OA. Synovial lining cell layer, resident cells in stroma and especially inflammatory infiltration were increasing with severity of OA. iNOS expression in both intima and subintima was positively correlated with Krenn score in moderate and severe osteoarthritis (OA) groups. Expression of BCL-2 in intima of severe OA patients was positively correlated with Krenn score. In conclusion, iNOS, BCL-2 and MMP-9 are involved in the regulation of HOA. Our study indicates a relationship between the pathohistological features, the synovial inflammation and the cartilage condition at the time of hip replacement due to OA or femoral neck fracture. |
| first_indexed | 2024-03-09T06:04:49Z |
| format | Article |
| id | doaj.art-dea78fbb569b42ba8dce6176cf4105a5 |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T06:04:49Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-dea78fbb569b42ba8dce6176cf4105a52023-12-03T12:06:13ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-02-01223148910.3390/ijms22031489Expression Pattern of iNOS, BCL-2 and MMP-9 in the Hip Synovium Tissue of Patients with OsteoarthritisDavor Caric0Sandra Zekic Tomas1Natalija Filipovic2Violeta Soljic3Benjamin Benzon4Sandro Glumac5Ivan Rakovac6Katarina Vukojevic7Department of Orthopaedics and Traumatology, University Hospital in Split, Spinciceva 1, 21000 Split, CroatiaDepartment of Pathology, Forensic Medicine and Cytology, University Hospital in Split, Spinciceva 1, 21000 Split, CroatiaDepartment of Anatomy, Histology and Embryology, School of Medicine, University of Split, Soltanska 2, 21000 Split, CroatiaDepartment of Histology and Embryology, School of Medicine, University of Mostar, Kralja Petra Kresimira IV, 88000 Mostar, Bosnia and HerzegovinaDepartment of Anatomy, Histology and Embryology, School of Medicine, University of Split, Soltanska 2, 21000 Split, CroatiaDepartment of Anesthesiology and Intensive Care, University Hospital in Split, Spinciceva 1, 21000 Split, CroatiaDepartment of Natural and Health Sciences, Juraj Dobrila University of Pula, Pula, Zagrebačka ul. 30, 52100 Pula, CroatiaDepartment of Anatomy, Histology and Embryology, School of Medicine, University of Split, Soltanska 2, 21000 Split, CroatiaHip osteoarthritis (HOA) is characterized by degradation of the cartilage and synovitis. However, the pathohistological effects of synovial tissue inflammation on HOA are not clear. The aim of this study was to evaluate the expression of iNOS, BCL-2 and MMP-9 markers in different synovial cell populations. A total of 32 patients were evaluated retrospectively. Age, sex, height, weight, body mass index were recorded and lymphocyte, fibrocytes and macrophages were analysed in tissue sections. Osteoarthritis cartilage histopathology assessment system (OARSI), Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), Krenn score, Harris Hip Score (HHS) and Kellgren–Lawrence (K-L) grading of the hip joints were performed. Total hip arthroplasty was performed on 32 patients and controls. Patients were divided into two groups according to their disease severity. The tissues were immunohistochemically analysed. K-L grade and Krenn score differ between all three groups, but also between moderate and severe OA. Synovial lining cell layer, resident cells in stroma and especially inflammatory infiltration were increasing with severity of OA. iNOS expression in both intima and subintima was positively correlated with Krenn score in moderate and severe osteoarthritis (OA) groups. Expression of BCL-2 in intima of severe OA patients was positively correlated with Krenn score. In conclusion, iNOS, BCL-2 and MMP-9 are involved in the regulation of HOA. Our study indicates a relationship between the pathohistological features, the synovial inflammation and the cartilage condition at the time of hip replacement due to OA or femoral neck fracture.https://www.mdpi.com/1422-0067/22/3/1489osteoarthritisHipiNOSBCL-2MMP-9cartilage |
| spellingShingle | Davor Caric Sandra Zekic Tomas Natalija Filipovic Violeta Soljic Benjamin Benzon Sandro Glumac Ivan Rakovac Katarina Vukojevic Expression Pattern of iNOS, BCL-2 and MMP-9 in the Hip Synovium Tissue of Patients with Osteoarthritis International Journal of Molecular Sciences osteoarthritis Hip iNOS BCL-2 MMP-9 cartilage |
| title | Expression Pattern of iNOS, BCL-2 and MMP-9 in the Hip Synovium Tissue of Patients with Osteoarthritis |
| title_full | Expression Pattern of iNOS, BCL-2 and MMP-9 in the Hip Synovium Tissue of Patients with Osteoarthritis |
| title_fullStr | Expression Pattern of iNOS, BCL-2 and MMP-9 in the Hip Synovium Tissue of Patients with Osteoarthritis |
| title_full_unstemmed | Expression Pattern of iNOS, BCL-2 and MMP-9 in the Hip Synovium Tissue of Patients with Osteoarthritis |
| title_short | Expression Pattern of iNOS, BCL-2 and MMP-9 in the Hip Synovium Tissue of Patients with Osteoarthritis |
| title_sort | expression pattern of inos bcl 2 and mmp 9 in the hip synovium tissue of patients with osteoarthritis |
| topic | osteoarthritis Hip iNOS BCL-2 MMP-9 cartilage |
| url | https://www.mdpi.com/1422-0067/22/3/1489 |
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