A simplified transposon mutagenesis method to perform phenotypic forward genetic screens in cultured cells
Abstract Background The introduction of genome-wide shRNA and CRISPR libraries has facilitated cell-based screens to identify loss-of-function mutations associated with a phenotype of interest. Approaches to perform analogous gain-of-function screens are less common, although some reports have utili...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2019-06-01
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| Series: | BMC Genomics |
| Online Access: | http://link.springer.com/article/10.1186/s12864-019-5888-6 |