Genome-wide identification and expression patterns in response to signals from cadmium of StCADs gene family in potato (Solanum tuberosum L.)
Abstract Background With the rapid development of the economy and society, soil pollution is becoming more and more serious. Heavy metal cadmium (Cd) pollution is one of the typical problems, which poses a potentially serious threat to crop production and human health. Cinnamyl alcohol dehydrogenase...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2024-02-01
|
| Series: | Chemical and Biological Technologies in Agriculture |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40538-024-00543-7 |
| _version_ | 1797275580306030592 |
|---|---|
| author | XinYu Yang HePing Lv Wu Zhang HongJie Liang YanPing Gao YiChen Kang YanBin Wu FangFang Wang Chunyan Xi |
| author_facet | XinYu Yang HePing Lv Wu Zhang HongJie Liang YanPing Gao YiChen Kang YanBin Wu FangFang Wang Chunyan Xi |
| author_sort | XinYu Yang |
| collection | DOAJ |
| description | Abstract Background With the rapid development of the economy and society, soil pollution is becoming more and more serious. Heavy metal cadmium (Cd) pollution is one of the typical problems, which poses a potentially serious threat to crop production and human health. Cinnamyl alcohol dehydrogenase (CAD) is a key enzyme in lignin synthesis and plays an important role in plant resistance to external stress. In this study, combined with bioinformatics analysis and expression pattern analysis, the members of the potato CAD family were identified, and their physical and chemical properties, evolutionary characteristics and chromosome location were clarified, as well as their regulatory effects on Cd tolerance. Results A total of 50 StCAD genes belonging to 6 subfamilies were obtained, and all of them were located in the cytoplasm. Members of the same family had similar gene structures and functional domains. The promoter region of each StCAD family member contains at least 5 or more abiotic stress response elements, indicating that the family had potential functions in regulating stress. According to the expression pattern analysis, most genes in this family were upregulated after Cd stress, further enhanced CAD activity and significantly promoted lignin accumulation in potato roots. Conclusion In summary, the StCAD family plays an important role in potato response to Cd stress. This study lays a foundation for further studies on the functions of the StCAD family and provides candidate genes for Cd resistance molecular breeding in potato. Graphical Abstract |
| first_indexed | 2024-03-07T15:16:29Z |
| format | Article |
| id | doaj.art-deb2d97f9a68451da9ec56d9b0409c4e |
| institution | Directory Open Access Journal |
| issn | 2196-5641 |
| language | English |
| last_indexed | 2024-03-07T15:16:29Z |
| publishDate | 2024-02-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Chemical and Biological Technologies in Agriculture |
| spelling | doaj.art-deb2d97f9a68451da9ec56d9b0409c4e2024-03-05T17:54:48ZengSpringerOpenChemical and Biological Technologies in Agriculture2196-56412024-02-0111111310.1186/s40538-024-00543-7Genome-wide identification and expression patterns in response to signals from cadmium of StCADs gene family in potato (Solanum tuberosum L.)XinYu Yang0HePing Lv1Wu Zhang2HongJie Liang3YanPing Gao4YiChen Kang5YanBin Wu6FangFang Wang7Chunyan Xi8Institute of Potato, Gansu Academy of Agricultural SciencesInstitute of Potato, Gansu Academy of Agricultural SciencesInstitute of Potato, Gansu Academy of Agricultural SciencesInstitute of Potato, Gansu Academy of Agricultural SciencesInstitute of Potato, Gansu Academy of Agricultural SciencesHorticulture College of Gansu Agricultural UniversityInstitute of Potato, Gansu Academy of Agricultural SciencesInstitute of Potato, Gansu Academy of Agricultural SciencesInstitute of Potato, Gansu Academy of Agricultural SciencesAbstract Background With the rapid development of the economy and society, soil pollution is becoming more and more serious. Heavy metal cadmium (Cd) pollution is one of the typical problems, which poses a potentially serious threat to crop production and human health. Cinnamyl alcohol dehydrogenase (CAD) is a key enzyme in lignin synthesis and plays an important role in plant resistance to external stress. In this study, combined with bioinformatics analysis and expression pattern analysis, the members of the potato CAD family were identified, and their physical and chemical properties, evolutionary characteristics and chromosome location were clarified, as well as their regulatory effects on Cd tolerance. Results A total of 50 StCAD genes belonging to 6 subfamilies were obtained, and all of them were located in the cytoplasm. Members of the same family had similar gene structures and functional domains. The promoter region of each StCAD family member contains at least 5 or more abiotic stress response elements, indicating that the family had potential functions in regulating stress. According to the expression pattern analysis, most genes in this family were upregulated after Cd stress, further enhanced CAD activity and significantly promoted lignin accumulation in potato roots. Conclusion In summary, the StCAD family plays an important role in potato response to Cd stress. This study lays a foundation for further studies on the functions of the StCAD family and provides candidate genes for Cd resistance molecular breeding in potato. Graphical Abstracthttps://doi.org/10.1186/s40538-024-00543-7Heavy metal stressCAD familyBioinformatics analysisGene expressive pattern |
| spellingShingle | XinYu Yang HePing Lv Wu Zhang HongJie Liang YanPing Gao YiChen Kang YanBin Wu FangFang Wang Chunyan Xi Genome-wide identification and expression patterns in response to signals from cadmium of StCADs gene family in potato (Solanum tuberosum L.) Chemical and Biological Technologies in Agriculture Heavy metal stress CAD family Bioinformatics analysis Gene expressive pattern |
| title | Genome-wide identification and expression patterns in response to signals from cadmium of StCADs gene family in potato (Solanum tuberosum L.) |
| title_full | Genome-wide identification and expression patterns in response to signals from cadmium of StCADs gene family in potato (Solanum tuberosum L.) |
| title_fullStr | Genome-wide identification and expression patterns in response to signals from cadmium of StCADs gene family in potato (Solanum tuberosum L.) |
| title_full_unstemmed | Genome-wide identification and expression patterns in response to signals from cadmium of StCADs gene family in potato (Solanum tuberosum L.) |
| title_short | Genome-wide identification and expression patterns in response to signals from cadmium of StCADs gene family in potato (Solanum tuberosum L.) |
| title_sort | genome wide identification and expression patterns in response to signals from cadmium of stcads gene family in potato solanum tuberosum l |
| topic | Heavy metal stress CAD family Bioinformatics analysis Gene expressive pattern |
| url | https://doi.org/10.1186/s40538-024-00543-7 |
| work_keys_str_mv | AT xinyuyang genomewideidentificationandexpressionpatternsinresponsetosignalsfromcadmiumofstcadsgenefamilyinpotatosolanumtuberosuml AT hepinglv genomewideidentificationandexpressionpatternsinresponsetosignalsfromcadmiumofstcadsgenefamilyinpotatosolanumtuberosuml AT wuzhang genomewideidentificationandexpressionpatternsinresponsetosignalsfromcadmiumofstcadsgenefamilyinpotatosolanumtuberosuml AT hongjieliang genomewideidentificationandexpressionpatternsinresponsetosignalsfromcadmiumofstcadsgenefamilyinpotatosolanumtuberosuml AT yanpinggao genomewideidentificationandexpressionpatternsinresponsetosignalsfromcadmiumofstcadsgenefamilyinpotatosolanumtuberosuml AT yichenkang genomewideidentificationandexpressionpatternsinresponsetosignalsfromcadmiumofstcadsgenefamilyinpotatosolanumtuberosuml AT yanbinwu genomewideidentificationandexpressionpatternsinresponsetosignalsfromcadmiumofstcadsgenefamilyinpotatosolanumtuberosuml AT fangfangwang genomewideidentificationandexpressionpatternsinresponsetosignalsfromcadmiumofstcadsgenefamilyinpotatosolanumtuberosuml AT chunyanxi genomewideidentificationandexpressionpatternsinresponsetosignalsfromcadmiumofstcadsgenefamilyinpotatosolanumtuberosuml |