Novel dandelion mannan-lipid nanoparticle: Exploring the molecular mechanism underlying the potent anticancer effect against non-small lung carcinoma
New β-branched glucomannan (TOPL) with an average molecular weight (4.29 × 104 Da) was purified from dandelion leaves for the first time. TOPL possesses a potent IC50 against non-small lung carcinoma (NSCLC); A549 and H460 cell lines (4.01 and 6.57 μg/ml), respectively. To improve the drug-like prop...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-12-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464621004308 |
| _version_ | 1818572831957450752 |
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| author | Soad Z. El-Emam Dina Moustafa Abo El-Ella Sahar M. Fayez Mohsen Asker Jilan A. Nazeam |
| author_facet | Soad Z. El-Emam Dina Moustafa Abo El-Ella Sahar M. Fayez Mohsen Asker Jilan A. Nazeam |
| author_sort | Soad Z. El-Emam |
| collection | DOAJ |
| description | New β-branched glucomannan (TOPL) with an average molecular weight (4.29 × 104 Da) was purified from dandelion leaves for the first time. TOPL possesses a potent IC50 against non-small lung carcinoma (NSCLC); A549 and H460 cell lines (4.01 and 6.57 μg/ml), respectively. To improve the drug-like properties of TOPL, enhance solubility, and counteract its poor bioavailability, the study aimed to design nano-functional particle (nTOPL) by using different nanocarriers such as liposomes and solid lipid nanoparticles (SLNs). Moreover, nTOPL exerted a marked cytotoxic effect against A549 (2.2 μg/ml), H460 (1.7 μg/ml), with minimal toxicity against normal WI-38 cells (18.0 μg/ml). Besides, nTOPL promoted apoptosis and induced arrest in the S-phase, G2/M phase, with up-regulation in Beclin-1, Bax thresholds, and contritely inhibition of Bcl-2 expression. In conclusion, the study implied that nTOPL is a new mannan-lipid nanoparticle that could provide a future promising candidate as a functional food and alternative medicine in lung cancer. |
| first_indexed | 2024-12-15T00:02:54Z |
| format | Article |
| id | doaj.art-deb3113bacb14f339e9cb5bc97654b38 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-15T00:02:54Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-deb3113bacb14f339e9cb5bc97654b382022-12-21T22:42:51ZengElsevierJournal of Functional Foods1756-46462021-12-0187104781Novel dandelion mannan-lipid nanoparticle: Exploring the molecular mechanism underlying the potent anticancer effect against non-small lung carcinomaSoad Z. El-Emam0Dina Moustafa Abo El-Ella1Sahar M. Fayez2Mohsen Asker3Jilan A. Nazeam4Pharmacology and Toxicology Department, October 6 University, Giza, Egypt; Corresponding authors at: Pharmacology and Toxicology, Faculty of Pharmacy, October 6 University, Giza, Egypt.Pharmacology and Toxicology Department, October 6 University, Giza, EgyptPharmaceutics and Industrial Pharmacy Department, October 6 University, Giza, EgyptMicrobial Biotechnology Department, National Research Centre, Dokki, Cairo, EgyptPharmacognosy Department, October 6 University, Giza, Egypt; Corresponding authors at: Pharmacology and Toxicology, Faculty of Pharmacy, October 6 University, Giza, Egypt.New β-branched glucomannan (TOPL) with an average molecular weight (4.29 × 104 Da) was purified from dandelion leaves for the first time. TOPL possesses a potent IC50 against non-small lung carcinoma (NSCLC); A549 and H460 cell lines (4.01 and 6.57 μg/ml), respectively. To improve the drug-like properties of TOPL, enhance solubility, and counteract its poor bioavailability, the study aimed to design nano-functional particle (nTOPL) by using different nanocarriers such as liposomes and solid lipid nanoparticles (SLNs). Moreover, nTOPL exerted a marked cytotoxic effect against A549 (2.2 μg/ml), H460 (1.7 μg/ml), with minimal toxicity against normal WI-38 cells (18.0 μg/ml). Besides, nTOPL promoted apoptosis and induced arrest in the S-phase, G2/M phase, with up-regulation in Beclin-1, Bax thresholds, and contritely inhibition of Bcl-2 expression. In conclusion, the study implied that nTOPL is a new mannan-lipid nanoparticle that could provide a future promising candidate as a functional food and alternative medicine in lung cancer.http://www.sciencedirect.com/science/article/pii/S1756464621004308Solid lipid nanoparticlesTaraxacum officinaleDandelionMannanAutophagyApoptosis |
| spellingShingle | Soad Z. El-Emam Dina Moustafa Abo El-Ella Sahar M. Fayez Mohsen Asker Jilan A. Nazeam Novel dandelion mannan-lipid nanoparticle: Exploring the molecular mechanism underlying the potent anticancer effect against non-small lung carcinoma Journal of Functional Foods Solid lipid nanoparticles Taraxacum officinale Dandelion Mannan Autophagy Apoptosis |
| title | Novel dandelion mannan-lipid nanoparticle: Exploring the molecular mechanism underlying the potent anticancer effect against non-small lung carcinoma |
| title_full | Novel dandelion mannan-lipid nanoparticle: Exploring the molecular mechanism underlying the potent anticancer effect against non-small lung carcinoma |
| title_fullStr | Novel dandelion mannan-lipid nanoparticle: Exploring the molecular mechanism underlying the potent anticancer effect against non-small lung carcinoma |
| title_full_unstemmed | Novel dandelion mannan-lipid nanoparticle: Exploring the molecular mechanism underlying the potent anticancer effect against non-small lung carcinoma |
| title_short | Novel dandelion mannan-lipid nanoparticle: Exploring the molecular mechanism underlying the potent anticancer effect against non-small lung carcinoma |
| title_sort | novel dandelion mannan lipid nanoparticle exploring the molecular mechanism underlying the potent anticancer effect against non small lung carcinoma |
| topic | Solid lipid nanoparticles Taraxacum officinale Dandelion Mannan Autophagy Apoptosis |
| url | http://www.sciencedirect.com/science/article/pii/S1756464621004308 |
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