Phylogenetic analysis of a swine influenza A(H3N2) virus isolated in Korea in 2012.
Influenza A virus (IAV) can infect avian and mammalian species, including humans. The genome nature of IAVs may contribute to viral adaptation in different animal hosts, resulting in gene reassortment and the reproduction of variants with optimal fitness. As seen again in the 2009 swine-origin influ...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3921248?pdf=render |
| _version_ | 1818316052649476096 |
|---|---|
| author | Jin Il Kim Ilseob Lee Sehee Park Sangmoo Lee Min-Woong Hwang Joon-Yong Bae Jun Heo Donghwan Kim Seok-Il Jang Kabsu Kim Man-Seong Park |
| author_facet | Jin Il Kim Ilseob Lee Sehee Park Sangmoo Lee Min-Woong Hwang Joon-Yong Bae Jun Heo Donghwan Kim Seok-Il Jang Kabsu Kim Man-Seong Park |
| author_sort | Jin Il Kim |
| collection | DOAJ |
| description | Influenza A virus (IAV) can infect avian and mammalian species, including humans. The genome nature of IAVs may contribute to viral adaptation in different animal hosts, resulting in gene reassortment and the reproduction of variants with optimal fitness. As seen again in the 2009 swine-origin influenza A H1N1 pandemic, pigs are known to be susceptible to swine, avian, and human IAVs and can serve as a 'mixing vessel' for the generation of novel IAV variants. To this end, the emergence of swine influenza viruses must be kept under close surveillance. Herein, we report the isolation and phylogenetic study of a swine IAV, A/swine/Korea/PL01/2012 (swPL01, H3N2 subtype). After screening nasopharyngeal samples from pigs in the Gyeongsangnam-do region of Korea from December 2011 to May 2012, we isolated the swPL01 virus and sequenced its all of 8 genome segments (polymerase basic 2, PB2; polymerase basic 1, PB1; polymerase acidic, PA; hemagglutinin, HA; nucleocapsid protein, NP; neuraminidase, NA; matrix protein, M; and nonstructural protein, NS). The phylogenetic study, analyzed with reference strains registered in the National Center for Biotechnology Information (NCBI) database, indicated that the swPL01 virus was similar to the North American triple-reassortant swine strains and that the HA gene of the swPL01 virus was categorized into swine H3 cluster IV. The swPL01 virus had the M gene of the triple-reassortant swine H3N2 viruses, whereas that of other contemporary strains in Korea was transferred from the 2009 pandemic H1N1 virus. These data suggest the possibility that various swine H3N2 viruses may co-circulate in Korea, which underlines the importance of a sustained surveillance system against swine IAVs. |
| first_indexed | 2024-12-13T09:15:18Z |
| format | Article |
| id | doaj.art-deb682115e314e6ea8480bc0aff363a0 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T09:15:18Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-deb682115e314e6ea8480bc0aff363a02022-12-21T23:52:52ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0192e8878210.1371/journal.pone.0088782Phylogenetic analysis of a swine influenza A(H3N2) virus isolated in Korea in 2012.Jin Il KimIlseob LeeSehee ParkSangmoo LeeMin-Woong HwangJoon-Yong BaeJun HeoDonghwan KimSeok-Il JangKabsu KimMan-Seong ParkInfluenza A virus (IAV) can infect avian and mammalian species, including humans. The genome nature of IAVs may contribute to viral adaptation in different animal hosts, resulting in gene reassortment and the reproduction of variants with optimal fitness. As seen again in the 2009 swine-origin influenza A H1N1 pandemic, pigs are known to be susceptible to swine, avian, and human IAVs and can serve as a 'mixing vessel' for the generation of novel IAV variants. To this end, the emergence of swine influenza viruses must be kept under close surveillance. Herein, we report the isolation and phylogenetic study of a swine IAV, A/swine/Korea/PL01/2012 (swPL01, H3N2 subtype). After screening nasopharyngeal samples from pigs in the Gyeongsangnam-do region of Korea from December 2011 to May 2012, we isolated the swPL01 virus and sequenced its all of 8 genome segments (polymerase basic 2, PB2; polymerase basic 1, PB1; polymerase acidic, PA; hemagglutinin, HA; nucleocapsid protein, NP; neuraminidase, NA; matrix protein, M; and nonstructural protein, NS). The phylogenetic study, analyzed with reference strains registered in the National Center for Biotechnology Information (NCBI) database, indicated that the swPL01 virus was similar to the North American triple-reassortant swine strains and that the HA gene of the swPL01 virus was categorized into swine H3 cluster IV. The swPL01 virus had the M gene of the triple-reassortant swine H3N2 viruses, whereas that of other contemporary strains in Korea was transferred from the 2009 pandemic H1N1 virus. These data suggest the possibility that various swine H3N2 viruses may co-circulate in Korea, which underlines the importance of a sustained surveillance system against swine IAVs.http://europepmc.org/articles/PMC3921248?pdf=render |
| spellingShingle | Jin Il Kim Ilseob Lee Sehee Park Sangmoo Lee Min-Woong Hwang Joon-Yong Bae Jun Heo Donghwan Kim Seok-Il Jang Kabsu Kim Man-Seong Park Phylogenetic analysis of a swine influenza A(H3N2) virus isolated in Korea in 2012. PLoS ONE |
| title | Phylogenetic analysis of a swine influenza A(H3N2) virus isolated in Korea in 2012. |
| title_full | Phylogenetic analysis of a swine influenza A(H3N2) virus isolated in Korea in 2012. |
| title_fullStr | Phylogenetic analysis of a swine influenza A(H3N2) virus isolated in Korea in 2012. |
| title_full_unstemmed | Phylogenetic analysis of a swine influenza A(H3N2) virus isolated in Korea in 2012. |
| title_short | Phylogenetic analysis of a swine influenza A(H3N2) virus isolated in Korea in 2012. |
| title_sort | phylogenetic analysis of a swine influenza a h3n2 virus isolated in korea in 2012 |
| url | http://europepmc.org/articles/PMC3921248?pdf=render |
| work_keys_str_mv | AT jinilkim phylogeneticanalysisofaswineinfluenzaah3n2virusisolatedinkoreain2012 AT ilseoblee phylogeneticanalysisofaswineinfluenzaah3n2virusisolatedinkoreain2012 AT seheepark phylogeneticanalysisofaswineinfluenzaah3n2virusisolatedinkoreain2012 AT sangmoolee phylogeneticanalysisofaswineinfluenzaah3n2virusisolatedinkoreain2012 AT minwoonghwang phylogeneticanalysisofaswineinfluenzaah3n2virusisolatedinkoreain2012 AT joonyongbae phylogeneticanalysisofaswineinfluenzaah3n2virusisolatedinkoreain2012 AT junheo phylogeneticanalysisofaswineinfluenzaah3n2virusisolatedinkoreain2012 AT donghwankim phylogeneticanalysisofaswineinfluenzaah3n2virusisolatedinkoreain2012 AT seokiljang phylogeneticanalysisofaswineinfluenzaah3n2virusisolatedinkoreain2012 AT kabsukim phylogeneticanalysisofaswineinfluenzaah3n2virusisolatedinkoreain2012 AT manseongpark phylogeneticanalysisofaswineinfluenzaah3n2virusisolatedinkoreain2012 |