Evolutionary biogeography on Ophiocordyceps sinensis: An indicator of molecular phylogeny to geochronological and ecological exchanges
The fungus Ophiocordyceps sinensis is endemic to the vast region of the Qinghai-Tibetan plateau (QTP). The unique and complex geographical environmental conditions have led to the “sky island” distribution structure of O. sinensis. Due to limited and unbalanced sample collections, the previous data...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-05-01
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| Series: | Geoscience Frontiers |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1674987119301641 |
| _version_ | 1797711612466954240 |
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| author | Yongdong Dai Changkui Wu Feng Yuan Yuanbing Wang Luodong Huang Zihong Chen Wenbo Zeng Yao Wang Zhuliang Yang Pusheng Zeng Paul Lemetti Xuanxue Mo Hong Yu |
| author_facet | Yongdong Dai Changkui Wu Feng Yuan Yuanbing Wang Luodong Huang Zihong Chen Wenbo Zeng Yao Wang Zhuliang Yang Pusheng Zeng Paul Lemetti Xuanxue Mo Hong Yu |
| author_sort | Yongdong Dai |
| collection | DOAJ |
| description | The fungus Ophiocordyceps sinensis is endemic to the vast region of the Qinghai-Tibetan plateau (QTP). The unique and complex geographical environmental conditions have led to the “sky island” distribution structure of O. sinensis. Due to limited and unbalanced sample collections, the previous data on O. sinensis regarding its genetic diversity and spatial structure have been deemed insufficient. In this study, we analyzed the diversity and phylogeographic structures of O. sinensis using internally transcribed spacer region (ITS) and 5-locus datasets by a large-scale sampling. A total of 111 haplotypes of ITS sequences were identified from 948 samples data of the fungus O. sinensis, with representing high genetic diversity, and 8 phylogenetic clades were recognized in O. sinensis. Both the southeastern Tibet and the northwestern Yunnan were the centers of genetic diversity and genetic differentiation of the fungus, and they were inferred as the glacial refugia in the Quaternary. Three distribution patterns were identified to correspond to the 8 clades, including but not limited to the coexistence of widely and specific local distributive structures. It also revealed that the differentiation pattern of O. sinensis did not fit for the isolation-by-distance model. The differentiation into the 8 clades occurred between 1.56 Myr and 6.62 Myr. The ancestor of O. sinensis most likely originated in the late Miocene (6.62 Myr) in the northwestern Yunnan, and the Scene A–C of the Qinghai–Tibetan movements may have played an important role in the differentiation of O. sinensis during the late Miocene–Pliocene periods. Our current results provide a much clearer and detailed understanding of the genetic diversity and geographical spatial distribution of the endemic alpine fungus O. sinensis. It also revealed that the geochronology resulting from paleogeology could be cross-examined with biomolecular clock at a finer scale. Keywords: Large-scale sampling, Phylogeographic structure, Molecular clock, Correlation, Qinghai–Tibetan plateau |
| first_indexed | 2024-03-12T07:09:37Z |
| format | Article |
| id | doaj.art-deb73ab5c77e40b3a91f77a45e7a3786 |
| institution | Directory Open Access Journal |
| issn | 1674-9871 |
| language | English |
| last_indexed | 2024-03-12T07:09:37Z |
| publishDate | 2020-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Geoscience Frontiers |
| spelling | doaj.art-deb73ab5c77e40b3a91f77a45e7a37862023-09-02T23:15:30ZengElsevierGeoscience Frontiers1674-98712020-05-01113807820Evolutionary biogeography on Ophiocordyceps sinensis: An indicator of molecular phylogeny to geochronological and ecological exchangesYongdong Dai0Changkui Wu1Feng Yuan2Yuanbing Wang3Luodong Huang4Zihong Chen5Wenbo Zeng6Yao Wang7Zhuliang Yang8Pusheng Zeng9Paul Lemetti10Xuanxue Mo11Hong Yu12Yunnan Herbal Laboratory, Institute of Herb Biotic Resources, School of Life Sciences, Yunnan University, Kunming, Yunnan Province 650091, ChinaYunnan Herbal Laboratory, Institute of Herb Biotic Resources, School of Life Sciences, Yunnan University, Kunming, Yunnan Province 650091, ChinaYunnan Herbal Laboratory, Institute of Herb Biotic Resources, School of Life Sciences, Yunnan University, Kunming, Yunnan Province 650091, ChinaYunnan Herbal Laboratory, Institute of Herb Biotic Resources, School of Life Sciences, Yunnan University, Kunming, Yunnan Province 650091, China; Yunnan Herbal Biotech Co., Ltd, Kunming, Yunnan Province 650203, ChinaYunnan Herbal Laboratory, Institute of Herb Biotic Resources, School of Life Sciences, Yunnan University, Kunming, Yunnan Province 650091, ChinaYunnan Herbal Laboratory, Institute of Herb Biotic Resources, School of Life Sciences, Yunnan University, Kunming, Yunnan Province 650091, ChinaYunnan Herbal Laboratory, Institute of Herb Biotic Resources, School of Life Sciences, Yunnan University, Kunming, Yunnan Province 650091, ChinaYunnan Herbal Laboratory, Institute of Herb Biotic Resources, School of Life Sciences, Yunnan University, Kunming, Yunnan Province 650091, ChinaKey Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan Province 650201, ChinaNational Research Center for Geoanalysis, Beijing 100037, ChinaYunnan Herbal Laboratory, Institute of Herb Biotic Resources, School of Life Sciences, Yunnan University, Kunming, Yunnan Province 650091, ChinaSchool of Earth Sciences and Resources, China University of Geosciences (Beijing), Beijing 100083, ChinaYunnan Herbal Laboratory, Institute of Herb Biotic Resources, School of Life Sciences, Yunnan University, Kunming, Yunnan Province 650091, China; Corresponding author. No. 2 Cuihu Lake North Road, Kunming, Yunnan, 650091, China.The fungus Ophiocordyceps sinensis is endemic to the vast region of the Qinghai-Tibetan plateau (QTP). The unique and complex geographical environmental conditions have led to the “sky island” distribution structure of O. sinensis. Due to limited and unbalanced sample collections, the previous data on O. sinensis regarding its genetic diversity and spatial structure have been deemed insufficient. In this study, we analyzed the diversity and phylogeographic structures of O. sinensis using internally transcribed spacer region (ITS) and 5-locus datasets by a large-scale sampling. A total of 111 haplotypes of ITS sequences were identified from 948 samples data of the fungus O. sinensis, with representing high genetic diversity, and 8 phylogenetic clades were recognized in O. sinensis. Both the southeastern Tibet and the northwestern Yunnan were the centers of genetic diversity and genetic differentiation of the fungus, and they were inferred as the glacial refugia in the Quaternary. Three distribution patterns were identified to correspond to the 8 clades, including but not limited to the coexistence of widely and specific local distributive structures. It also revealed that the differentiation pattern of O. sinensis did not fit for the isolation-by-distance model. The differentiation into the 8 clades occurred between 1.56 Myr and 6.62 Myr. The ancestor of O. sinensis most likely originated in the late Miocene (6.62 Myr) in the northwestern Yunnan, and the Scene A–C of the Qinghai–Tibetan movements may have played an important role in the differentiation of O. sinensis during the late Miocene–Pliocene periods. Our current results provide a much clearer and detailed understanding of the genetic diversity and geographical spatial distribution of the endemic alpine fungus O. sinensis. It also revealed that the geochronology resulting from paleogeology could be cross-examined with biomolecular clock at a finer scale. Keywords: Large-scale sampling, Phylogeographic structure, Molecular clock, Correlation, Qinghai–Tibetan plateauhttp://www.sciencedirect.com/science/article/pii/S1674987119301641 |
| spellingShingle | Yongdong Dai Changkui Wu Feng Yuan Yuanbing Wang Luodong Huang Zihong Chen Wenbo Zeng Yao Wang Zhuliang Yang Pusheng Zeng Paul Lemetti Xuanxue Mo Hong Yu Evolutionary biogeography on Ophiocordyceps sinensis: An indicator of molecular phylogeny to geochronological and ecological exchanges Geoscience Frontiers |
| title | Evolutionary biogeography on Ophiocordyceps sinensis: An indicator of molecular phylogeny to geochronological and ecological exchanges |
| title_full | Evolutionary biogeography on Ophiocordyceps sinensis: An indicator of molecular phylogeny to geochronological and ecological exchanges |
| title_fullStr | Evolutionary biogeography on Ophiocordyceps sinensis: An indicator of molecular phylogeny to geochronological and ecological exchanges |
| title_full_unstemmed | Evolutionary biogeography on Ophiocordyceps sinensis: An indicator of molecular phylogeny to geochronological and ecological exchanges |
| title_short | Evolutionary biogeography on Ophiocordyceps sinensis: An indicator of molecular phylogeny to geochronological and ecological exchanges |
| title_sort | evolutionary biogeography on ophiocordyceps sinensis an indicator of molecular phylogeny to geochronological and ecological exchanges |
| url | http://www.sciencedirect.com/science/article/pii/S1674987119301641 |
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