Mosquito CYP4C21 knockout reduces dengue virus and Zika virus replication in Aedes aegypti cells

Mosquito CYP4C21 knockout reduces dengue virus and Zika virus replication in Aedes aegypti cells

Aedes aegypti (Ae. aegypti) is a major vector of dengue virus (DENV) and Zika virus (ZIKV). Understanding the complex interaction mechanisms between mosquito vectors and arboviruses is essential to interrupt virus transmission. This study constructed CYP4C21 knockout (KO) Aag2 cells (Ae. aegypti cel...

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Bibliographic Details
Main Authors: Xiaoxue Xie, Di Wang, Bo Li, Manjin Li, Dan Xing, Teng Zhao, Xinyu Zhou, Chunxiao Li
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:Biosafety and Health
Subjects:
CYP4C21
Aag2 cell
DENV2
ZIKV
CRISPR/Cas9
Online Access:http://www.sciencedirect.com/science/article/pii/S2590053623000496
Description
Summary:Aedes aegypti (Ae. aegypti) is a major vector of dengue virus (DENV) and Zika virus (ZIKV). Understanding the complex interaction mechanisms between mosquito vectors and arboviruses is essential to interrupt virus transmission. This study constructed CYP4C21 knockout (KO) Aag2 cells (Ae. aegypti cells) and confirmed that CYP4C21 KO reduced DENV2 and ZIKV copies in Aag2 cells, which suggests that CYP4C21 may play an important role in mosquito infection with arboviruses. Furthermore, it is the first report of the CYP4 family related to viral infection, which lays the foundation for exploring the role of the CYP4C21 in the interaction of Ae. aegypti and arbovirus and provides novel insights into the function of cytochrome family proteins.
ISSN:2590-0536

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