Evaluation Of Immune Response To Brucella Infection In Sheep
Brucellosis in sheep and goats is a zoonotic infection (excluding Brucellaovis) with important effects on both public health and animal health. The disease is widespread in many areas of the world, particularly in some Mediterranean and Middle Easterncountries. Aim of the study: the present study...
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| Format: | Article |
| Language: | English |
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Faculty of Veterinary Medicine, University of Kufa
2015-06-01
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| Series: | Kufa Journal for Veterinary Medical Sciences |
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| Online Access: | https://journal.uokufa.edu.iq/index.php/kjvs/article/view/4018 |
| _version_ | 1797364043622645760 |
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| author | Kareem Mohamed Abdullah O Alhatami Akram Motlak |
| author_facet | Kareem Mohamed Abdullah O Alhatami Akram Motlak |
| author_sort | Kareem Mohamed |
| collection | DOAJ |
| description |
Brucellosis in sheep and goats is a zoonotic infection (excluding Brucellaovis) with important effects on both public health and animal health. The disease is widespread in many areas of the world, particularly in some Mediterranean and Middle Easterncountries.
Aim of the study: the present study aims to find out the prevalence of brucellosis in sheep in one of the areas of An Najaf El-Ashraf city, and study of the immune response by measuring the levels of and interleukin-10 (IL-10).
Methodology: Current study conducted in the Najaf province (Al-Barakyia) during the period between September 2013- March 2014, fulfilled by using Rose Bengal and Enzyme Immunosorbent Assay (ELISA) tests for estimation of brucella antibodies. A total number of 48 serum samples were collected from sheep and stored at -20 C.
Results: the frequency of positive sera by Rose Bengal test (RBT) was 20% and by ELISA technique was 25%. The level of interleukin 10 (IL-10) was estimated in all surveyed animals, 12 sera samples were showed an elevated IL-10 levels, all were positive with ELISA serological test.
Conclusion: the detection of significant concentration of IL-10 among the seropositive cases may be indicated an active infection of brucella with type 2 cytokine production.
Recommendations: the evaluation of immune response of vaccinated sheep with brucella REV1 vaccine, depending on the estimation of IFN-γ and IL-10 ratio that will reflect the cytokines induced during vaccination and consequently, the effective or not immune response.
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| first_indexed | 2024-03-08T16:29:41Z |
| format | Article |
| id | doaj.art-deb9e145289845e59421c5a3c5f9e6b9 |
| institution | Directory Open Access Journal |
| issn | 2077-9798 2959-8478 |
| language | English |
| last_indexed | 2024-03-08T16:29:41Z |
| publishDate | 2015-06-01 |
| publisher | Faculty of Veterinary Medicine, University of Kufa |
| record_format | Article |
| series | Kufa Journal for Veterinary Medical Sciences |
| spelling | doaj.art-deb9e145289845e59421c5a3c5f9e6b92024-01-06T10:21:49ZengFaculty of Veterinary Medicine, University of KufaKufa Journal for Veterinary Medical Sciences2077-97982959-84782015-06-016110.36326/kjvs/2015/v6i14018Evaluation Of Immune Response To Brucella Infection In SheepKareem MohamedAbdullah O Alhatamihttps://orcid.org/0000-0002-4656-7657Akram Motlak Brucellosis in sheep and goats is a zoonotic infection (excluding Brucellaovis) with important effects on both public health and animal health. The disease is widespread in many areas of the world, particularly in some Mediterranean and Middle Easterncountries. Aim of the study: the present study aims to find out the prevalence of brucellosis in sheep in one of the areas of An Najaf El-Ashraf city, and study of the immune response by measuring the levels of and interleukin-10 (IL-10). Methodology: Current study conducted in the Najaf province (Al-Barakyia) during the period between September 2013- March 2014, fulfilled by using Rose Bengal and Enzyme Immunosorbent Assay (ELISA) tests for estimation of brucella antibodies. A total number of 48 serum samples were collected from sheep and stored at -20 C. Results: the frequency of positive sera by Rose Bengal test (RBT) was 20% and by ELISA technique was 25%. The level of interleukin 10 (IL-10) was estimated in all surveyed animals, 12 sera samples were showed an elevated IL-10 levels, all were positive with ELISA serological test. Conclusion: the detection of significant concentration of IL-10 among the seropositive cases may be indicated an active infection of brucella with type 2 cytokine production. Recommendations: the evaluation of immune response of vaccinated sheep with brucella REV1 vaccine, depending on the estimation of IFN-γ and IL-10 ratio that will reflect the cytokines induced during vaccination and consequently, the effective or not immune response. https://journal.uokufa.edu.iq/index.php/kjvs/article/view/4018BrucellosisELISAInterleukin 10Rose Bengalsheep |
| spellingShingle | Kareem Mohamed Abdullah O Alhatami Akram Motlak Evaluation Of Immune Response To Brucella Infection In Sheep Kufa Journal for Veterinary Medical Sciences Brucellosis ELISA Interleukin 10 Rose Bengal sheep |
| title | Evaluation Of Immune Response To Brucella Infection In Sheep |
| title_full | Evaluation Of Immune Response To Brucella Infection In Sheep |
| title_fullStr | Evaluation Of Immune Response To Brucella Infection In Sheep |
| title_full_unstemmed | Evaluation Of Immune Response To Brucella Infection In Sheep |
| title_short | Evaluation Of Immune Response To Brucella Infection In Sheep |
| title_sort | evaluation of immune response to brucella infection in sheep |
| topic | Brucellosis ELISA Interleukin 10 Rose Bengal sheep |
| url | https://journal.uokufa.edu.iq/index.php/kjvs/article/view/4018 |
| work_keys_str_mv | AT kareemmohamed evaluationofimmuneresponsetobrucellainfectioninsheep AT abdullahoalhatami evaluationofimmuneresponsetobrucellainfectioninsheep AT akrammotlak evaluationofimmuneresponsetobrucellainfectioninsheep |