In vitro growth of Cattleya nobilior Rchb. f.: culture media, sealing systems and irradiance
Some factors may influence the in vitro culture of native orchids, especially the culture medium, sealing system and light condition. This study aimed to evaluate the addition of banana pulp and peel in the formulation of culture media, sealing system and irradiance, in the in vitro growth of Ca...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Goiás
2021-03-01
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| Series: | Pesquisa Agropecuária Tropical |
| Subjects: | |
| Online Access: | https://www.revistas.ufg.br/pat/article/view/67131/36468 |
| _version_ | 1798029205987917824 |
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| author | Kaliana Gottschalk de Freitas José Carlos Sorgato Jackeline Schultz Soares Luan Marlon Ribeiro |
| author_facet | Kaliana Gottschalk de Freitas José Carlos Sorgato Jackeline Schultz Soares Luan Marlon Ribeiro |
| author_sort | Kaliana Gottschalk de Freitas |
| collection | DOAJ |
| description | Some factors may influence the in vitro culture of native orchids, especially the culture medium, sealing system and light condition. This study aimed to evaluate the addition of banana pulp and peel in the formulation of culture media, sealing system and irradiance, in the in vitro growth of Cattleya nobilior Rchb. f. A completely randomized design was used in a factorial scheme with five culture media [Murashige & Skoog supplemented or not with banana pulp or peel (100 or 200g L-1)], two light conditions [3,000 K LED lamps (43 μmol m-2 s-1 or 86 μmol m-2 s-1)] and two sealing systems (natural and conventional ventilation), with six replicates of one vial each. The plant height, diameter of the largest pseudobulb, length of the largest root and of the largest leaf, fresh weight and number of leaves, roots and shoots were evaluated. The use of both the banana pulp and peel as additives in the culture medium formulation promoted the C. nobilior growth. The natural ventilation system increased the plant height, pseudobulb diameter and leaf length, while the conventional system promoted tillering. |
| first_indexed | 2024-04-11T19:20:31Z |
| format | Article |
| id | doaj.art-debaa0a85bdc48038f4826b2d767a91d |
| institution | Directory Open Access Journal |
| issn | 1983-4063 |
| language | English |
| last_indexed | 2024-04-11T19:20:31Z |
| publishDate | 2021-03-01 |
| publisher | Universidade Federal de Goiás |
| record_format | Article |
| series | Pesquisa Agropecuária Tropical |
| spelling | doaj.art-debaa0a85bdc48038f4826b2d767a91d2022-12-22T04:07:19ZengUniversidade Federal de GoiásPesquisa Agropecuária Tropical1983-40632021-03-0151e6713110.1590/1983-40632021v5167131In vitro growth of Cattleya nobilior Rchb. f.: culture media, sealing systems and irradianceKaliana Gottschalk de Freitas0https://orcid.org/0000-0003-2223-3676José Carlos Sorgato1https://orcid.org/0000-0003-0001-5467Jackeline Schultz Soares2https://orcid.org/0000-0001-9497-2975Luan Marlon Ribeiro3https://orcid.org/0000-0002-7278-7696Universidade Federal da Grande Dourados, Faculdade de Ciências Agrárias, Dourados, MS, Brasil.Universidade Federal da Grande Dourados, Faculdade de Ciências Agrárias, Dourados, MS, Brasil.Universidade Federal da Grande Dourados, Faculdade de Ciências Agrárias, Dourados, MS, Brasil.Universidade Federal da Grande Dourados, Faculdade de Ciências Agrárias, Dourados, MS, Brasil.Some factors may influence the in vitro culture of native orchids, especially the culture medium, sealing system and light condition. This study aimed to evaluate the addition of banana pulp and peel in the formulation of culture media, sealing system and irradiance, in the in vitro growth of Cattleya nobilior Rchb. f. A completely randomized design was used in a factorial scheme with five culture media [Murashige & Skoog supplemented or not with banana pulp or peel (100 or 200g L-1)], two light conditions [3,000 K LED lamps (43 μmol m-2 s-1 or 86 μmol m-2 s-1)] and two sealing systems (natural and conventional ventilation), with six replicates of one vial each. The plant height, diameter of the largest pseudobulb, length of the largest root and of the largest leaf, fresh weight and number of leaves, roots and shoots were evaluated. The use of both the banana pulp and peel as additives in the culture medium formulation promoted the C. nobilior growth. The natural ventilation system increased the plant height, pseudobulb diameter and leaf length, while the conventional system promoted tillering.https://www.revistas.ufg.br/pat/article/view/67131/36468orchidaceaeornamental horticulturemicropropagationnative plantbrazilian savanna |
| spellingShingle | Kaliana Gottschalk de Freitas José Carlos Sorgato Jackeline Schultz Soares Luan Marlon Ribeiro In vitro growth of Cattleya nobilior Rchb. f.: culture media, sealing systems and irradiance Pesquisa Agropecuária Tropical orchidaceae ornamental horticulture micropropagation native plant brazilian savanna |
| title | In vitro growth of Cattleya nobilior Rchb. f.: culture media, sealing systems and irradiance |
| title_full | In vitro growth of Cattleya nobilior Rchb. f.: culture media, sealing systems and irradiance |
| title_fullStr | In vitro growth of Cattleya nobilior Rchb. f.: culture media, sealing systems and irradiance |
| title_full_unstemmed | In vitro growth of Cattleya nobilior Rchb. f.: culture media, sealing systems and irradiance |
| title_short | In vitro growth of Cattleya nobilior Rchb. f.: culture media, sealing systems and irradiance |
| title_sort | in vitro growth of cattleya nobilior rchb f culture media sealing systems and irradiance |
| topic | orchidaceae ornamental horticulture micropropagation native plant brazilian savanna |
| url | https://www.revistas.ufg.br/pat/article/view/67131/36468 |
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