Implications of lysyl oxidase-like protein 3 expression in the periodontium of diabetic rats
Abstract Objectives Diabetes has been strongly associated with periodontal diseases. The periodontal ligament (PDL) has an abundant extracellular matrix (ECM). Lysyl oxidases (LOXs) are closely associated with various diseases caused by abnormal ECM functions, however, the role of LOXs in periodon...
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| Format: | Article |
| Language: | English |
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University of São Paulo
2022-09-01
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| Series: | Journal of Applied Oral Science |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572022000100438&tlng=en |
| _version_ | 1828377917064740864 |
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| author | Li HUANG Jun CHEN Yuling ZUO Jinle LI Yuan YUE Min WANG Liang HAO |
| author_facet | Li HUANG Jun CHEN Yuling ZUO Jinle LI Yuan YUE Min WANG Liang HAO |
| author_sort | Li HUANG |
| collection | DOAJ |
| description | Abstract Objectives Diabetes has been strongly associated with periodontal diseases. The periodontal ligament (PDL) has an abundant extracellular matrix (ECM). Lysyl oxidases (LOXs) are closely associated with various diseases caused by abnormal ECM functions, however, the role of LOXs in periodontal diseases induced by diabetes remains unclear. Methodology In this study, 8-week-old Zucker diabetic fatty rats were used to establish a type 2 diabetes mellitus (T2DM) model. After 9 and 16 weeks, hematoxylin and eosin (H&E), Masson’s trichrome, and immunohistochemical staining were performed. Results After 9 weeks, loose collagen fibers were found in the interradicular area of the diabetic group, in opposition to the control group. There were no significant differences in LOX expression between the diabetic and control groups (p>0.05). However, after 16 weeks, the diabetic group presented a disordered arrangement of the PDL, showing decreased collagen content and significantly increased lysyl oxidase-like protein 3 (LOXL3) expression when compared with the control group (p<0.05). This suggests that LOXL3 plays a significant role in periodontal histopathological changes in diabetic rats. Conclusion Our study showed elevated LOXL3 expression in the PDL of diabetic rats after 16 weeks, suggesting that LOXL3 may be involved in the occurrence and development of periodontal histopathological changes in diabetic rats. LOXL3 could be further used as an indicator for the early diagnosis of diabetic periodontitis in T2DM patients in clinical settings. |
| first_indexed | 2024-04-14T08:18:45Z |
| format | Article |
| id | doaj.art-debc7050e86d42f68adddf8fb5c50eb9 |
| institution | Directory Open Access Journal |
| issn | 1678-7765 |
| language | English |
| last_indexed | 2024-04-14T08:18:45Z |
| publishDate | 2022-09-01 |
| publisher | University of São Paulo |
| record_format | Article |
| series | Journal of Applied Oral Science |
| spelling | doaj.art-debc7050e86d42f68adddf8fb5c50eb92022-12-22T02:04:19ZengUniversity of São PauloJournal of Applied Oral Science1678-77652022-09-013010.1590/1678-7757-2022-0176Implications of lysyl oxidase-like protein 3 expression in the periodontium of diabetic ratsLi HUANGhttps://orcid.org/0000-0002-2534-2926Jun CHENhttps://orcid.org/0000-0003-1403-9806Yuling ZUOhttps://orcid.org/0000-0003-2084-5097Jinle LIhttps://orcid.org/0000-0001-5305-6639Yuan YUEhttps://orcid.org/0000-0002-3076-4971Min WANGhttps://orcid.org/0000-0003-1791-2631Liang HAOhttps://orcid.org/0000-0001-7207-9131Abstract Objectives Diabetes has been strongly associated with periodontal diseases. The periodontal ligament (PDL) has an abundant extracellular matrix (ECM). Lysyl oxidases (LOXs) are closely associated with various diseases caused by abnormal ECM functions, however, the role of LOXs in periodontal diseases induced by diabetes remains unclear. Methodology In this study, 8-week-old Zucker diabetic fatty rats were used to establish a type 2 diabetes mellitus (T2DM) model. After 9 and 16 weeks, hematoxylin and eosin (H&E), Masson’s trichrome, and immunohistochemical staining were performed. Results After 9 weeks, loose collagen fibers were found in the interradicular area of the diabetic group, in opposition to the control group. There were no significant differences in LOX expression between the diabetic and control groups (p>0.05). However, after 16 weeks, the diabetic group presented a disordered arrangement of the PDL, showing decreased collagen content and significantly increased lysyl oxidase-like protein 3 (LOXL3) expression when compared with the control group (p<0.05). This suggests that LOXL3 plays a significant role in periodontal histopathological changes in diabetic rats. Conclusion Our study showed elevated LOXL3 expression in the PDL of diabetic rats after 16 weeks, suggesting that LOXL3 may be involved in the occurrence and development of periodontal histopathological changes in diabetic rats. LOXL3 could be further used as an indicator for the early diagnosis of diabetic periodontitis in T2DM patients in clinical settings.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572022000100438&tlng=enLysyl oxidasesPeriodontal ligamentDiabetesPeriodontal diseasesLOXL3 |
| spellingShingle | Li HUANG Jun CHEN Yuling ZUO Jinle LI Yuan YUE Min WANG Liang HAO Implications of lysyl oxidase-like protein 3 expression in the periodontium of diabetic rats Journal of Applied Oral Science Lysyl oxidases Periodontal ligament Diabetes Periodontal diseases LOXL3 |
| title | Implications of lysyl oxidase-like protein 3 expression in the periodontium of diabetic rats |
| title_full | Implications of lysyl oxidase-like protein 3 expression in the periodontium of diabetic rats |
| title_fullStr | Implications of lysyl oxidase-like protein 3 expression in the periodontium of diabetic rats |
| title_full_unstemmed | Implications of lysyl oxidase-like protein 3 expression in the periodontium of diabetic rats |
| title_short | Implications of lysyl oxidase-like protein 3 expression in the periodontium of diabetic rats |
| title_sort | implications of lysyl oxidase like protein 3 expression in the periodontium of diabetic rats |
| topic | Lysyl oxidases Periodontal ligament Diabetes Periodontal diseases LOXL3 |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572022000100438&tlng=en |
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