Effects of delayed NSAID administration after experimental eccentric contraction injury - A cellular and proteomics study.
BACKGROUND:Acute muscle injuries are exceedingly common and non-steroidal anti-inflammatory drugs (NSAIDs) are widely consumed to reduce the associated inflammation, swelling and pain that peak 1-2 days post-injury. While prophylactic use or early administration of NSAIDs has been shown to delay mus...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5330483?pdf=render |
| _version_ | 1798043243005345792 |
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| author | Amy E Bryant Michael J Aldape Clifford R Bayer Eva J Katahira Laura Bond Carrie D Nicora Thomas L Fillmore Therese R W Clauss Thomas O Metz Bobbie-Jo Webb-Robertson Dennis L Stevens |
| author_facet | Amy E Bryant Michael J Aldape Clifford R Bayer Eva J Katahira Laura Bond Carrie D Nicora Thomas L Fillmore Therese R W Clauss Thomas O Metz Bobbie-Jo Webb-Robertson Dennis L Stevens |
| author_sort | Amy E Bryant |
| collection | DOAJ |
| description | BACKGROUND:Acute muscle injuries are exceedingly common and non-steroidal anti-inflammatory drugs (NSAIDs) are widely consumed to reduce the associated inflammation, swelling and pain that peak 1-2 days post-injury. While prophylactic use or early administration of NSAIDs has been shown to delay muscle regeneration and contribute to loss of muscle strength after healing, little is known about the effects of delayed NSAID use. Further, NSAID use following non-penetrating injury has been associated with increased risk and severity of infection, including that due to group A streptococcus, though the mechanisms remain to be elucidated. The present study investigated the effects of delayed NSAID administration on muscle repair and sought mechanisms supporting an injury/NSAID/infection axis. METHODS:A murine model of eccentric contraction (EC)-induced injury of the tibialis anterior muscle was used to profile the cellular and molecular changes induced by ketorolac tromethamine administered 47 hr post injury. RESULTS:NSAID administration inhibited several important muscle regeneration processes and down-regulated multiple cytoprotective proteins known to inhibit the intrinsic pathway of programmed cell death. These activities were associated with increased caspase activity in injured muscles but were independent of any NSAID effect on macrophage influx or phenotype switching. CONCLUSIONS:These findings provide new molecular evidence supporting the notion that NSAIDs have a direct negative influence on muscle repair after acute strain injury in mice and thus add to renewed concern about the safety and benefits of NSAIDS in both children and adults, in those with progressive loss of muscle mass such as the elderly or patients with cancer or AIDS, and those at risk of secondary infection after trauma or surgery. |
| first_indexed | 2024-04-11T22:46:39Z |
| format | Article |
| id | doaj.art-debe4290161745cba72e49bf78a41bbd |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-11T22:46:39Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-debe4290161745cba72e49bf78a41bbd2022-12-22T03:58:44ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01122e017248610.1371/journal.pone.0172486Effects of delayed NSAID administration after experimental eccentric contraction injury - A cellular and proteomics study.Amy E BryantMichael J AldapeClifford R BayerEva J KatahiraLaura BondCarrie D NicoraThomas L FillmoreTherese R W ClaussThomas O MetzBobbie-Jo Webb-RobertsonDennis L StevensBACKGROUND:Acute muscle injuries are exceedingly common and non-steroidal anti-inflammatory drugs (NSAIDs) are widely consumed to reduce the associated inflammation, swelling and pain that peak 1-2 days post-injury. While prophylactic use or early administration of NSAIDs has been shown to delay muscle regeneration and contribute to loss of muscle strength after healing, little is known about the effects of delayed NSAID use. Further, NSAID use following non-penetrating injury has been associated with increased risk and severity of infection, including that due to group A streptococcus, though the mechanisms remain to be elucidated. The present study investigated the effects of delayed NSAID administration on muscle repair and sought mechanisms supporting an injury/NSAID/infection axis. METHODS:A murine model of eccentric contraction (EC)-induced injury of the tibialis anterior muscle was used to profile the cellular and molecular changes induced by ketorolac tromethamine administered 47 hr post injury. RESULTS:NSAID administration inhibited several important muscle regeneration processes and down-regulated multiple cytoprotective proteins known to inhibit the intrinsic pathway of programmed cell death. These activities were associated with increased caspase activity in injured muscles but were independent of any NSAID effect on macrophage influx or phenotype switching. CONCLUSIONS:These findings provide new molecular evidence supporting the notion that NSAIDs have a direct negative influence on muscle repair after acute strain injury in mice and thus add to renewed concern about the safety and benefits of NSAIDS in both children and adults, in those with progressive loss of muscle mass such as the elderly or patients with cancer or AIDS, and those at risk of secondary infection after trauma or surgery.http://europepmc.org/articles/PMC5330483?pdf=render |
| spellingShingle | Amy E Bryant Michael J Aldape Clifford R Bayer Eva J Katahira Laura Bond Carrie D Nicora Thomas L Fillmore Therese R W Clauss Thomas O Metz Bobbie-Jo Webb-Robertson Dennis L Stevens Effects of delayed NSAID administration after experimental eccentric contraction injury - A cellular and proteomics study. PLoS ONE |
| title | Effects of delayed NSAID administration after experimental eccentric contraction injury - A cellular and proteomics study. |
| title_full | Effects of delayed NSAID administration after experimental eccentric contraction injury - A cellular and proteomics study. |
| title_fullStr | Effects of delayed NSAID administration after experimental eccentric contraction injury - A cellular and proteomics study. |
| title_full_unstemmed | Effects of delayed NSAID administration after experimental eccentric contraction injury - A cellular and proteomics study. |
| title_short | Effects of delayed NSAID administration after experimental eccentric contraction injury - A cellular and proteomics study. |
| title_sort | effects of delayed nsaid administration after experimental eccentric contraction injury a cellular and proteomics study |
| url | http://europepmc.org/articles/PMC5330483?pdf=render |
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