Serum-free Culture of Chicken Bursal Epithelial Cells
We developed a method of culturing bursal epithelial cells under serum-free condition. Bursal cells sampled from 14 days of embryogenesis were cultured in DMEM/Ham’s F12 1 : 1 mixture medium supplemented with epidermal growth factor, insulin, transferrin, sodium selenite, hydrocortisone a...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Japan Poultry Science Association
2003-01-01
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| Series: | The Journal of Poultry Science |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/40/1/40_1_69/_pdf/-char/en |
| _version_ | 1797816971226513408 |
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| author | Song Han Eiichiro Mitsusada Asaki Abe Yasuhiro Kondo |
| author_facet | Song Han Eiichiro Mitsusada Asaki Abe Yasuhiro Kondo |
| author_sort | Song Han |
| collection | DOAJ |
| description | We developed a method of culturing bursal epithelial cells under serum-free condition. Bursal cells sampled from 14 days of embryogenesis were cultured in DMEM/Ham’s F12 1 : 1 mixture medium supplemented with epidermal growth factor, insulin, transferrin, sodium selenite, hydrocortisone and 2-aminoethanol. A monolayer, in which polygonal cells were extensively observed, entirely covered the well after 2 to 3 weeks of incubation. The cultured cells were maintained for 5 months without any apparent morphological abnormalities. Polygonal cells in wells cultured for 3 weeks or 5 months were reacted with anti-keratin antibody, indicating these cells are cytokeratin-positive cells. These results suggest that most cells proliferated under a serum-free condition are epithelial cells. It seems possible to precisely analyze presumable factors in the supernatant of bursal epithelial cell culture. |
| first_indexed | 2024-03-13T08:45:32Z |
| format | Article |
| id | doaj.art-dec12f4784eb40eeb9029d6d7dd1413e |
| institution | Directory Open Access Journal |
| issn | 1346-7395 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:45:32Z |
| publishDate | 2003-01-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-dec12f4784eb40eeb9029d6d7dd1413e2023-05-30T05:46:31ZengJapan Poultry Science AssociationThe Journal of Poultry Science1346-73951349-04862003-01-01401697310.2141/jpsa.40.69jpsaSerum-free Culture of Chicken Bursal Epithelial CellsSong Han0Eiichiro Mitsusada1Asaki Abe2Yasuhiro Kondo3Faculty of Agriculture, Okayama UniversityFaculty of Agriculture, Okayama UniversityFaculty of Agriculture, Okayama UniversityFaculty of Agriculture, Okayama UniversityWe developed a method of culturing bursal epithelial cells under serum-free condition. Bursal cells sampled from 14 days of embryogenesis were cultured in DMEM/Ham’s F12 1 : 1 mixture medium supplemented with epidermal growth factor, insulin, transferrin, sodium selenite, hydrocortisone and 2-aminoethanol. A monolayer, in which polygonal cells were extensively observed, entirely covered the well after 2 to 3 weeks of incubation. The cultured cells were maintained for 5 months without any apparent morphological abnormalities. Polygonal cells in wells cultured for 3 weeks or 5 months were reacted with anti-keratin antibody, indicating these cells are cytokeratin-positive cells. These results suggest that most cells proliferated under a serum-free condition are epithelial cells. It seems possible to precisely analyze presumable factors in the supernatant of bursal epithelial cell culture.https://www.jstage.jst.go.jp/article/jpsa/40/1/40_1_69/_pdf/-char/enbursa of fabriciusepithelial cellserum-free culture |
| spellingShingle | Song Han Eiichiro Mitsusada Asaki Abe Yasuhiro Kondo Serum-free Culture of Chicken Bursal Epithelial Cells The Journal of Poultry Science bursa of fabricius epithelial cell serum-free culture |
| title | Serum-free Culture of Chicken Bursal Epithelial Cells |
| title_full | Serum-free Culture of Chicken Bursal Epithelial Cells |
| title_fullStr | Serum-free Culture of Chicken Bursal Epithelial Cells |
| title_full_unstemmed | Serum-free Culture of Chicken Bursal Epithelial Cells |
| title_short | Serum-free Culture of Chicken Bursal Epithelial Cells |
| title_sort | serum free culture of chicken bursal epithelial cells |
| topic | bursa of fabricius epithelial cell serum-free culture |
| url | https://www.jstage.jst.go.jp/article/jpsa/40/1/40_1_69/_pdf/-char/en |
| work_keys_str_mv | AT songhan serumfreecultureofchickenbursalepithelialcells AT eiichiromitsusada serumfreecultureofchickenbursalepithelialcells AT asakiabe serumfreecultureofchickenbursalepithelialcells AT yasuhirokondo serumfreecultureofchickenbursalepithelialcells |