Investigating the Effects of Ellagic Acid on Thioacetamide-Induced Acute Liver Damage and Subsequent Encephalopathy in Rats
Background and purpose: Ellagic acid is a natural polyphenol compound with potential antioxidant properties that is present in most fruits, seeds, and vegetables. Therefore, this study was designed to investigate the effects of ellagic acid administration on the liver damage model caused by thioacet...
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences
2023-11-01
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| Series: | Journal of Mazandaran University of Medical Sciences |
| Subjects: | |
| Online Access: | http://jmums.mazums.ac.ir/article-1-19184-en.pdf |
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| author | Afarin Joudaki nezhad Hamidreza Mohammadi Farhad Momeni Morteza Amraei Ahmad Adineh |
| author_facet | Afarin Joudaki nezhad Hamidreza Mohammadi Farhad Momeni Morteza Amraei Ahmad Adineh |
| author_sort | Afarin Joudaki nezhad |
| collection | DOAJ |
| description | Background and purpose: Ellagic acid is a natural polyphenol compound with potential antioxidant properties that is present in most fruits, seeds, and vegetables. Therefore, this study was designed to investigate the effects of ellagic acid administration on the liver damage model caused by thioacetamide in rats.
Materials and methods: In this experimental study, 60 male Wistar rats weighing approximately 200 grams were randomly divided into six groups (n=10). Liver damage was caused by a single dose (100 mg/kg) administration of thioacetamide for three consecutive days. Different doses of ellagic acid (10, 25 and 50 mg/kg) were administered to the sick mice as a single dose for three consecutive days. Finally, 24 hours after the last injection, biochemical factors (ALP, AST, ALT, LDH, bilirubin, total protein), oxidative stress markers (LPO, ROS, FRAP, GSH), ammonium ion concentration, and movement activity of the mice were examined. One-way analysis of variance and Tukey's post hoc test were used for statistical analysis.
Results: Thioacetamide led to a significant increase in serum biochemical factors and oxidative stress markers in the studied groups (P<0.05). In addition, the amount of ammonium ion in the plasma, as a key effective factor in hepatic encephalopathy, was greatly increased in animals receiving thioacetamide (P<0.05). Administration of ellagic acid in different doses significantly reduced liver damage and plasma ammonium concentration (P<0.05).
Conclusion: The results of this study showed that ellagic acid reduced liver damage caused by thioacetamide through its antioxidant activities. |
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| format | Article |
| id | doaj.art-dec18360d7e84f10a19af8b218d3272a |
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| issn | 1735-9260 1735-9279 |
| language | English |
| last_indexed | 2024-03-10T18:18:21Z |
| publishDate | 2023-11-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Journal of Mazandaran University of Medical Sciences |
| spelling | doaj.art-dec18360d7e84f10a19af8b218d3272a2023-11-20T07:33:34ZengMazandaran University of Medical SciencesJournal of Mazandaran University of Medical Sciences1735-92601735-92792023-11-0133226157163Investigating the Effects of Ellagic Acid on Thioacetamide-Induced Acute Liver Damage and Subsequent Encephalopathy in RatsAfarin Joudaki nezhad0Hamidreza Mohammadi1Farhad Momeni2Morteza Amraei3Ahmad Adineh4 Pharmacy Student, Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran Assistant Professor, Department of Toxicology, Faculty of Pharmacy, Razi Herbal Medicine Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran Associate Professor, Department of Pharmacognosy and Pharmaceutical Biotechnology, Faculty of Pharmacy, Lorestan University of Medical Sciences, Khorramabad, Iran Pharmacist, Food and Drug Administration of Lorestan University of Medical Sciences, Khorramabad, Iran Assistant Professor, Department of Toxicology, Faculty of Pharmacy, Razi Herbal Medicine Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran Background and purpose: Ellagic acid is a natural polyphenol compound with potential antioxidant properties that is present in most fruits, seeds, and vegetables. Therefore, this study was designed to investigate the effects of ellagic acid administration on the liver damage model caused by thioacetamide in rats. Materials and methods: In this experimental study, 60 male Wistar rats weighing approximately 200 grams were randomly divided into six groups (n=10). Liver damage was caused by a single dose (100 mg/kg) administration of thioacetamide for three consecutive days. Different doses of ellagic acid (10, 25 and 50 mg/kg) were administered to the sick mice as a single dose for three consecutive days. Finally, 24 hours after the last injection, biochemical factors (ALP, AST, ALT, LDH, bilirubin, total protein), oxidative stress markers (LPO, ROS, FRAP, GSH), ammonium ion concentration, and movement activity of the mice were examined. One-way analysis of variance and Tukey's post hoc test were used for statistical analysis. Results: Thioacetamide led to a significant increase in serum biochemical factors and oxidative stress markers in the studied groups (P<0.05). In addition, the amount of ammonium ion in the plasma, as a key effective factor in hepatic encephalopathy, was greatly increased in animals receiving thioacetamide (P<0.05). Administration of ellagic acid in different doses significantly reduced liver damage and plasma ammonium concentration (P<0.05). Conclusion: The results of this study showed that ellagic acid reduced liver damage caused by thioacetamide through its antioxidant activities.http://jmums.mazums.ac.ir/article-1-19184-en.pdfthioacetamideliver damageplasma ammoniumellagic acidoxidative stress |
| spellingShingle | Afarin Joudaki nezhad Hamidreza Mohammadi Farhad Momeni Morteza Amraei Ahmad Adineh Investigating the Effects of Ellagic Acid on Thioacetamide-Induced Acute Liver Damage and Subsequent Encephalopathy in Rats Journal of Mazandaran University of Medical Sciences thioacetamide liver damage plasma ammonium ellagic acid oxidative stress |
| title | Investigating the Effects of Ellagic Acid on Thioacetamide-Induced Acute Liver Damage and Subsequent Encephalopathy in Rats |
| title_full | Investigating the Effects of Ellagic Acid on Thioacetamide-Induced Acute Liver Damage and Subsequent Encephalopathy in Rats |
| title_fullStr | Investigating the Effects of Ellagic Acid on Thioacetamide-Induced Acute Liver Damage and Subsequent Encephalopathy in Rats |
| title_full_unstemmed | Investigating the Effects of Ellagic Acid on Thioacetamide-Induced Acute Liver Damage and Subsequent Encephalopathy in Rats |
| title_short | Investigating the Effects of Ellagic Acid on Thioacetamide-Induced Acute Liver Damage and Subsequent Encephalopathy in Rats |
| title_sort | investigating the effects of ellagic acid on thioacetamide induced acute liver damage and subsequent encephalopathy in rats |
| topic | thioacetamide liver damage plasma ammonium ellagic acid oxidative stress |
| url | http://jmums.mazums.ac.ir/article-1-19184-en.pdf |
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