Genetic and Phenotypic Characterisation of a <i>Saccharomyces cerevisiae</i> Population of ‘Merwah’ White Wine
The study of yeast biodiversity represents an important step in the preservation of the local heritage, and this work in particular has an innovative character since no further studies have investigated ‘Merwah’, one of the main grape varieties used in winemaking in Lebanon. To g...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-10-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/7/11/492 |
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| author | Nadine Feghali Warren Albertin Edouard Tabet Ziad Rizk Angela Bianco Giacomo Zara Isabelle Masneuf-Pomarede Marilena Budroni |
| author_facet | Nadine Feghali Warren Albertin Edouard Tabet Ziad Rizk Angela Bianco Giacomo Zara Isabelle Masneuf-Pomarede Marilena Budroni |
| author_sort | Nadine Feghali |
| collection | DOAJ |
| description | The study of yeast biodiversity represents an important step in the preservation of the local heritage, and this work in particular has an innovative character since no further studies have investigated ‘Merwah’, one of the main grape varieties used in winemaking in Lebanon. To gain deeper knowledge of the genetic diversity and population structure of native <i>Saccharomyces cerevisiae</i> wine strains, 202 isolates were collected during spontaneous alcoholic fermentation of eight must/wine samples of cultivar ‘Merwah’, over two consecutive years (2016, 2017) in a traditional winery in Mount Lebanon (1400 m a.s.l.). The isolates were identified as <i>S. cerevisiae</i> on the basis of their morphology and preliminary sequence analysis of their internal transcribed spacer (ITS) PCR. They were then characterised at the strain level by interdelta PCR and genotyped using multiplex PCR reactions of 12 microsatellite markers. High genetic diversity was observed for the studied population. To select potential yeast starter strains from this population, micro-fermentations were carried out for 22 <i>S. cerevisiae</i> strains that were selected as representative of the ‘Merwah’ wine yeast population in order to determine their technological and oenological properties. Three indigenous yeast strains might represent candidates for pilot-scale fermentation in the winery, based on relevant features such as high fermentation vigour, low production of volatile acidity and H<sub>2</sub>S and low residual sugar content at the end of alcoholic fermentation. |
| first_indexed | 2024-12-12T21:33:02Z |
| format | Article |
| id | doaj.art-dec711ff01624374bc0061ac9ac13676 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-12-12T21:33:02Z |
| publishDate | 2019-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-dec711ff01624374bc0061ac9ac136762022-12-22T00:11:17ZengMDPI AGMicroorganisms2076-26072019-10-0171149210.3390/microorganisms7110492microorganisms7110492Genetic and Phenotypic Characterisation of a <i>Saccharomyces cerevisiae</i> Population of ‘Merwah’ White WineNadine Feghali0Warren Albertin1Edouard Tabet2Ziad Rizk3Angela Bianco4Giacomo Zara5Isabelle Masneuf-Pomarede6Marilena Budroni7Department of Agricultural Science, University of Sassari, 07100 Sassari, ItalyUR Œnologie EA 4577, USC 1366 INRA, University of Bordeaux, Villenave d’Ornon, 33882 Bordeaux, FranceFaculty of Agricultural Sciences-CRFA, Lebanese University, Ghazir, LebanonLebanese Agricultural Research Institute (LARI), 90-1965 Fanar, LebanonDepartment of Agricultural Science, University of Sassari, 07100 Sassari, ItalyDepartment of Agricultural Science, University of Sassari, 07100 Sassari, ItalyUR Œnologie EA 4577, USC 1366 INRA, University of Bordeaux, Villenave d’Ornon, 33882 Bordeaux, FranceDepartment of Agricultural Science, University of Sassari, 07100 Sassari, ItalyThe study of yeast biodiversity represents an important step in the preservation of the local heritage, and this work in particular has an innovative character since no further studies have investigated ‘Merwah’, one of the main grape varieties used in winemaking in Lebanon. To gain deeper knowledge of the genetic diversity and population structure of native <i>Saccharomyces cerevisiae</i> wine strains, 202 isolates were collected during spontaneous alcoholic fermentation of eight must/wine samples of cultivar ‘Merwah’, over two consecutive years (2016, 2017) in a traditional winery in Mount Lebanon (1400 m a.s.l.). The isolates were identified as <i>S. cerevisiae</i> on the basis of their morphology and preliminary sequence analysis of their internal transcribed spacer (ITS) PCR. They were then characterised at the strain level by interdelta PCR and genotyped using multiplex PCR reactions of 12 microsatellite markers. High genetic diversity was observed for the studied population. To select potential yeast starter strains from this population, micro-fermentations were carried out for 22 <i>S. cerevisiae</i> strains that were selected as representative of the ‘Merwah’ wine yeast population in order to determine their technological and oenological properties. Three indigenous yeast strains might represent candidates for pilot-scale fermentation in the winery, based on relevant features such as high fermentation vigour, low production of volatile acidity and H<sub>2</sub>S and low residual sugar content at the end of alcoholic fermentation.https://www.mdpi.com/2076-2607/7/11/492native yeastspontaneous fermentationgenotype–phenotype diversityregional wine |
| spellingShingle | Nadine Feghali Warren Albertin Edouard Tabet Ziad Rizk Angela Bianco Giacomo Zara Isabelle Masneuf-Pomarede Marilena Budroni Genetic and Phenotypic Characterisation of a <i>Saccharomyces cerevisiae</i> Population of ‘Merwah’ White Wine Microorganisms native yeast spontaneous fermentation genotype–phenotype diversity regional wine |
| title | Genetic and Phenotypic Characterisation of a <i>Saccharomyces cerevisiae</i> Population of ‘Merwah’ White Wine |
| title_full | Genetic and Phenotypic Characterisation of a <i>Saccharomyces cerevisiae</i> Population of ‘Merwah’ White Wine |
| title_fullStr | Genetic and Phenotypic Characterisation of a <i>Saccharomyces cerevisiae</i> Population of ‘Merwah’ White Wine |
| title_full_unstemmed | Genetic and Phenotypic Characterisation of a <i>Saccharomyces cerevisiae</i> Population of ‘Merwah’ White Wine |
| title_short | Genetic and Phenotypic Characterisation of a <i>Saccharomyces cerevisiae</i> Population of ‘Merwah’ White Wine |
| title_sort | genetic and phenotypic characterisation of a i saccharomyces cerevisiae i population of merwah white wine |
| topic | native yeast spontaneous fermentation genotype–phenotype diversity regional wine |
| url | https://www.mdpi.com/2076-2607/7/11/492 |
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