Genotyping and Antimicrobial Susceptibility Profiling of <i>Streptococcus uberis</i> Isolated from a Clinical Bovine Mastitis Outbreak in a Dairy Farm
<i>Streptococcus uberis</i>, an environmental pathogen responsible also for contagious transmission, has been increasingly implicated in clinical mastitis (CM) cases in Europe. We described a 4-month epidemiological investigation of <i>Strep. uberis</i> CM cases in an Italian...
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MDPI AG
2021-05-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/10/6/644 |
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| author | Valentina Monistero Antonio Barberio Paola Cremonesi Bianca Castiglioni Stefano Morandi Desiree C. K. Lassen Lærke B. Astrup Clara Locatelli Renata Piccinini M. Filippa Addis Valerio Bronzo Paolo Moroni |
| author_facet | Valentina Monistero Antonio Barberio Paola Cremonesi Bianca Castiglioni Stefano Morandi Desiree C. K. Lassen Lærke B. Astrup Clara Locatelli Renata Piccinini M. Filippa Addis Valerio Bronzo Paolo Moroni |
| author_sort | Valentina Monistero |
| collection | DOAJ |
| description | <i>Streptococcus uberis</i>, an environmental pathogen responsible also for contagious transmission, has been increasingly implicated in clinical mastitis (CM) cases in Europe. We described a 4-month epidemiological investigation of <i>Strep. uberis</i> CM cases in an Italian dairy farm. We determined molecular characteristics and phenotypic antimicrobial resistance of 71 <i>Strep. uberis</i> isolates from dairy cows with CM. Genotypic variability was investigated via multiplex PCR of housekeeping and virulence genes, and by RAPD-PCR typing. Antimicrobial susceptibility was assessed for 14 antimicrobials by MIC assay. All the isolates carried the 11 genes investigated. At 90% similarity, two distinct clusters, grouping 69 of the 71 isolates, were detected in the dendrogram derived from the primer ERIC1. The predominant cluster I could be separated into two subclusters, containing 38 and 14 isolates, respectively. <i>Strep. uberis</i> strains belonging to the same RAPD pattern differed in their resistance profiles. Most (97.2%) of them were resistant to at least one of the drugs tested, but only 25.4% showed a multidrug resistance phenotype. The highest resistance rate was observed for lincomycin (93%), followed by tetracycline (85.9%). This study confirmed a low prevalence of β-lactam resistance in <i>Strep. uberis,</i> with only one isolate showing resistance to six antimicrobial classes, including cephalosporins. |
| first_indexed | 2024-03-10T10:57:59Z |
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| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T10:57:59Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-dec7793e9e6d41b6bdbc5576ad3a5a962023-11-21T21:47:43ZengMDPI AGAntibiotics2079-63822021-05-0110664410.3390/antibiotics10060644Genotyping and Antimicrobial Susceptibility Profiling of <i>Streptococcus uberis</i> Isolated from a Clinical Bovine Mastitis Outbreak in a Dairy FarmValentina Monistero0Antonio Barberio1Paola Cremonesi2Bianca Castiglioni3Stefano Morandi4Desiree C. K. Lassen5Lærke B. Astrup6Clara Locatelli7Renata Piccinini8M. Filippa Addis9Valerio Bronzo10Paolo Moroni11Dipartimento di Medicina Veterinaria, Università degli Studi di Milano, 26900 Lodi, ItalyIstituto Zooprofilattico Sperimentale delle Venezie, 35020 Legnaro, ItalyInstitute of Agricultural Biology and Biotechnology, National Research Council, 26900 Lodi, ItalyInstitute of Agricultural Biology and Biotechnology, National Research Council, 26900 Lodi, ItalyInstitute of Sciences of Food Production, Italian National Research Council, 20133 Milan, ItalyCentre for Diagnostics, DTU Health Tech, Technical University of Denmark, 2800 Kongens Lyngby, DenmarkCentre for Diagnostics, DTU Health Tech, Technical University of Denmark, 2800 Kongens Lyngby, DenmarkDipartimento di Medicina Veterinaria, Università degli Studi di Milano, 26900 Lodi, ItalyDipartimento di Medicina Veterinaria, Università degli Studi di Milano, 26900 Lodi, ItalyDipartimento di Medicina Veterinaria, Università degli Studi di Milano, 26900 Lodi, ItalyDipartimento di Medicina Veterinaria, Università degli Studi di Milano, 26900 Lodi, ItalyDipartimento di Medicina Veterinaria, Università degli Studi di Milano, 26900 Lodi, Italy<i>Streptococcus uberis</i>, an environmental pathogen responsible also for contagious transmission, has been increasingly implicated in clinical mastitis (CM) cases in Europe. We described a 4-month epidemiological investigation of <i>Strep. uberis</i> CM cases in an Italian dairy farm. We determined molecular characteristics and phenotypic antimicrobial resistance of 71 <i>Strep. uberis</i> isolates from dairy cows with CM. Genotypic variability was investigated via multiplex PCR of housekeeping and virulence genes, and by RAPD-PCR typing. Antimicrobial susceptibility was assessed for 14 antimicrobials by MIC assay. All the isolates carried the 11 genes investigated. At 90% similarity, two distinct clusters, grouping 69 of the 71 isolates, were detected in the dendrogram derived from the primer ERIC1. The predominant cluster I could be separated into two subclusters, containing 38 and 14 isolates, respectively. <i>Strep. uberis</i> strains belonging to the same RAPD pattern differed in their resistance profiles. Most (97.2%) of them were resistant to at least one of the drugs tested, but only 25.4% showed a multidrug resistance phenotype. The highest resistance rate was observed for lincomycin (93%), followed by tetracycline (85.9%). This study confirmed a low prevalence of β-lactam resistance in <i>Strep. uberis,</i> with only one isolate showing resistance to six antimicrobial classes, including cephalosporins.https://www.mdpi.com/2079-6382/10/6/644<i>Streptococcus uberis</i>cowclinical mastitisRAPDMIC |
| spellingShingle | Valentina Monistero Antonio Barberio Paola Cremonesi Bianca Castiglioni Stefano Morandi Desiree C. K. Lassen Lærke B. Astrup Clara Locatelli Renata Piccinini M. Filippa Addis Valerio Bronzo Paolo Moroni Genotyping and Antimicrobial Susceptibility Profiling of <i>Streptococcus uberis</i> Isolated from a Clinical Bovine Mastitis Outbreak in a Dairy Farm Antibiotics <i>Streptococcus uberis</i> cow clinical mastitis RAPD MIC |
| title | Genotyping and Antimicrobial Susceptibility Profiling of <i>Streptococcus uberis</i> Isolated from a Clinical Bovine Mastitis Outbreak in a Dairy Farm |
| title_full | Genotyping and Antimicrobial Susceptibility Profiling of <i>Streptococcus uberis</i> Isolated from a Clinical Bovine Mastitis Outbreak in a Dairy Farm |
| title_fullStr | Genotyping and Antimicrobial Susceptibility Profiling of <i>Streptococcus uberis</i> Isolated from a Clinical Bovine Mastitis Outbreak in a Dairy Farm |
| title_full_unstemmed | Genotyping and Antimicrobial Susceptibility Profiling of <i>Streptococcus uberis</i> Isolated from a Clinical Bovine Mastitis Outbreak in a Dairy Farm |
| title_short | Genotyping and Antimicrobial Susceptibility Profiling of <i>Streptococcus uberis</i> Isolated from a Clinical Bovine Mastitis Outbreak in a Dairy Farm |
| title_sort | genotyping and antimicrobial susceptibility profiling of i streptococcus uberis i isolated from a clinical bovine mastitis outbreak in a dairy farm |
| topic | <i>Streptococcus uberis</i> cow clinical mastitis RAPD MIC |
| url | https://www.mdpi.com/2079-6382/10/6/644 |
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