The role of toll-like receptor 9 (TLR9) in Epstein-Barr virus-associated gastric cancer
Epstein-Barr virus-associated gastric carcinoma (EBVaGC) is the most common malignancy caused by EBV infection. Toll-like receptors (TLRs) as major components of innate immune system are crucial in the development of inflammatory processes and carcinogenesis. The aim of our study was to evaluate tis...
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| Format: | Article |
| Language: | English |
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Sciendo
2020-06-01
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| Series: | Current Issues in Pharmacy and Medical Sciences |
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| Online Access: | https://doi.org/10.2478/cipms-2020-0020 |
| _version_ | 1818668938498670592 |
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| author | Dworzanska Anna Strycharz-Dudziak Malgorzata Dworzanski Jakub Stec Agnieszka Rajtar Barbara Drop Bartlomiej Polz-Dacewicz Malgorzata |
| author_facet | Dworzanska Anna Strycharz-Dudziak Malgorzata Dworzanski Jakub Stec Agnieszka Rajtar Barbara Drop Bartlomiej Polz-Dacewicz Malgorzata |
| author_sort | Dworzanska Anna |
| collection | DOAJ |
| description | Epstein-Barr virus-associated gastric carcinoma (EBVaGC) is the most common malignancy caused by EBV infection. Toll-like receptors (TLRs) as major components of innate immune system are crucial in the development of inflammatory processes and carcinogenesis. The aim of our study was to evaluate tissue and serum level of TLR9 in EBV-positive and EBV-negative gastric cancer patients. The study involved 30 EBV(+) and 30 EBV(-) patients. EBV DNA was detected in fresh frozen tumor tissue. In serum samples TLR9 level, transforming growth factor β (TGFβ), interleukin 10 (IL-10) and antibodies against EBV were detected using ELISA tests. TLR9 level was also measured in homogenate of tumour tissue. TLR9 level was statistically lower in EBV(+) patients both in serum and tissue, with statistically higher level in tissue than in serum. Lower level of TLR9 was accompanied by higher level of Epstein-Barr virus capsid antigen (EBVCA), Epstein-Barr virus nuclear antigen (EBNA) and early antigen (EA). A lower level of TLR9 was detected in patients with poorly differentiated cancer (G3) and greater lymph nodes involvement (N3-N4). Lower level of TLR9 in patients with EA may point to TLR9 role in reactivation of EBV infection. |
| first_indexed | 2024-12-17T06:44:16Z |
| format | Article |
| id | doaj.art-dec7f55e86dd478981ffcee28ffe9685 |
| institution | Directory Open Access Journal |
| issn | 2084-980X 2300-6676 |
| language | English |
| last_indexed | 2024-12-17T06:44:16Z |
| publishDate | 2020-06-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Current Issues in Pharmacy and Medical Sciences |
| spelling | doaj.art-dec7f55e86dd478981ffcee28ffe96852022-12-21T21:59:47ZengSciendoCurrent Issues in Pharmacy and Medical Sciences2084-980X2300-66762020-06-0133210611110.2478/cipms-2020-0020cipms-2020-0020The role of toll-like receptor 9 (TLR9) in Epstein-Barr virus-associated gastric cancerDworzanska Anna0Strycharz-Dudziak Malgorzata1Dworzanski Jakub2Stec Agnieszka3Rajtar Barbara4Drop Bartlomiej5Polz-Dacewicz Malgorzata6Masovian Specialist Hospital,Radom, PolandChair and Department of Conservative Dentistry with Endodontics, Medical University of Lublin, PolandMasovian Specialist Hospital,Radom, PolandDepartment of Virology, Medical University of Lublin, PolandDepartment of Virology, Medical University of Lublin, PolandDepartment of Information Technology and Medical Statistics, Medical University of Lublin, PolandDepartment of Virology, Medical University of Lublin, PolandEpstein-Barr virus-associated gastric carcinoma (EBVaGC) is the most common malignancy caused by EBV infection. Toll-like receptors (TLRs) as major components of innate immune system are crucial in the development of inflammatory processes and carcinogenesis. The aim of our study was to evaluate tissue and serum level of TLR9 in EBV-positive and EBV-negative gastric cancer patients. The study involved 30 EBV(+) and 30 EBV(-) patients. EBV DNA was detected in fresh frozen tumor tissue. In serum samples TLR9 level, transforming growth factor β (TGFβ), interleukin 10 (IL-10) and antibodies against EBV were detected using ELISA tests. TLR9 level was also measured in homogenate of tumour tissue. TLR9 level was statistically lower in EBV(+) patients both in serum and tissue, with statistically higher level in tissue than in serum. Lower level of TLR9 was accompanied by higher level of Epstein-Barr virus capsid antigen (EBVCA), Epstein-Barr virus nuclear antigen (EBNA) and early antigen (EA). A lower level of TLR9 was detected in patients with poorly differentiated cancer (G3) and greater lymph nodes involvement (N3-N4). Lower level of TLR9 in patients with EA may point to TLR9 role in reactivation of EBV infection.https://doi.org/10.2478/cipms-2020-0020gastric cancertlr9ebvil-10tgfβ |
| spellingShingle | Dworzanska Anna Strycharz-Dudziak Malgorzata Dworzanski Jakub Stec Agnieszka Rajtar Barbara Drop Bartlomiej Polz-Dacewicz Malgorzata The role of toll-like receptor 9 (TLR9) in Epstein-Barr virus-associated gastric cancer Current Issues in Pharmacy and Medical Sciences gastric cancer tlr9 ebv il-10 tgfβ |
| title | The role of toll-like receptor 9 (TLR9) in Epstein-Barr virus-associated gastric cancer |
| title_full | The role of toll-like receptor 9 (TLR9) in Epstein-Barr virus-associated gastric cancer |
| title_fullStr | The role of toll-like receptor 9 (TLR9) in Epstein-Barr virus-associated gastric cancer |
| title_full_unstemmed | The role of toll-like receptor 9 (TLR9) in Epstein-Barr virus-associated gastric cancer |
| title_short | The role of toll-like receptor 9 (TLR9) in Epstein-Barr virus-associated gastric cancer |
| title_sort | role of toll like receptor 9 tlr9 in epstein barr virus associated gastric cancer |
| topic | gastric cancer tlr9 ebv il-10 tgfβ |
| url | https://doi.org/10.2478/cipms-2020-0020 |
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