Effect of resveratrol on SNARE proteins expression and insulin resistance in skeletal muscle of diabetic rats
<em><strong>Objective(s):</strong></em> Soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex proteins are involved in membrane trafficking. The expression of isoforms of SNAP-23, syntaxin-4, and VAMP-2 is significantly done in skeletal muscles...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2019-12-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | http://ijbms.mums.ac.ir/article_13988_e93b36c65dab92ba1b7dca92dd73787b.pdf |
| _version_ | 1811319734194405376 |
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| author | Azam Rezaei Farimani Mohammad Taghi Goodarzi Massoud Saidijam Reza Yadgar Azari Sadegh Zarei Soheila Asadi |
| author_facet | Azam Rezaei Farimani Mohammad Taghi Goodarzi Massoud Saidijam Reza Yadgar Azari Sadegh Zarei Soheila Asadi |
| author_sort | Azam Rezaei Farimani |
| collection | DOAJ |
| description | <em><strong>Objective(s):</strong></em> Soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex proteins are involved in membrane trafficking. The expression of isoforms of SNAP-23, syntaxin-4, and VAMP-2 is significantly done in skeletal muscles; they control GLUT4 trafficking. It is believed that type 2 diabetes could be caused by the modifications in the expression of SNARE complex proteins. The purpose of this study was to evaluate the effect of resveratrol on the expression of these proteins in type 2 diabetes.<br /><em><strong>Materials and Methods:</strong></em> Forty male Wistar rats were selected. Streptozotocin and nicotinamide were applied for the induction of type 2 diabetes. The animals were divided into five groups. Healthy and diabetic groups were set as control; resveratrol (1, 5, and 10 mg/kg body weight) was applied to treat the three groups of diabetic rats for 30 days. Real-time qRT-PCR was applied to evaluate the expression of SNARE complex proteins.<br /><em><strong>Results:</strong></em> There is a link between diabetes and insulin resistance and up-regulation of SNARE proteins expression. Resveratrol improved hyperglycemia and insulin resistance along with a non-significant reduction in the expression of SNARE proteins.<br /><em><strong>Conclusion:</strong></em> Increased expression of SNARE proteins was possibly a compensatory mechanism in response to insulin resistance in the skeletal muscles of diabetic rats. Resveratrol non-significantly reduced the expression of SNARE proteins by enhancing insulin sensitivity, where this effect was dose-dependent. Thus, higher doses of resveratrol and longer intervention periods could probably be more effective. Another molecular mechanism of the anti-diabetic properties of resveratrol was identified with an effect on the expression of SNARE proteins. |
| first_indexed | 2024-04-13T12:47:49Z |
| format | Article |
| id | doaj.art-decdbdd850a94bc0ba3976811ba90c65 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-04-13T12:47:49Z |
| publishDate | 2019-12-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-decdbdd850a94bc0ba3976811ba90c652022-12-22T02:46:19ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742019-12-0122121408141410.22038/ijbms.2019.1398813988Effect of resveratrol on SNARE proteins expression and insulin resistance in skeletal muscle of diabetic ratsAzam Rezaei Farimani0Mohammad Taghi Goodarzi1Massoud Saidijam2Reza Yadgar Azari3Sadegh Zarei4Soheila Asadi5Noncommunicable Diseases Research Center, Neyshabur University of Medical Sciences, Neyshabur, Iran|Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, IranResearch Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, IranResearch Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, IranDepartment of Molecular Medicine and Genetics, Medical School, Hamadan University of Medical Sciences, Hamadan, IranDepartment of Clinical Biochemistry, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, IranDepartment of Clinical Biochemistry, Facultyl of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran<em><strong>Objective(s):</strong></em> Soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex proteins are involved in membrane trafficking. The expression of isoforms of SNAP-23, syntaxin-4, and VAMP-2 is significantly done in skeletal muscles; they control GLUT4 trafficking. It is believed that type 2 diabetes could be caused by the modifications in the expression of SNARE complex proteins. The purpose of this study was to evaluate the effect of resveratrol on the expression of these proteins in type 2 diabetes.<br /><em><strong>Materials and Methods:</strong></em> Forty male Wistar rats were selected. Streptozotocin and nicotinamide were applied for the induction of type 2 diabetes. The animals were divided into five groups. Healthy and diabetic groups were set as control; resveratrol (1, 5, and 10 mg/kg body weight) was applied to treat the three groups of diabetic rats for 30 days. Real-time qRT-PCR was applied to evaluate the expression of SNARE complex proteins.<br /><em><strong>Results:</strong></em> There is a link between diabetes and insulin resistance and up-regulation of SNARE proteins expression. Resveratrol improved hyperglycemia and insulin resistance along with a non-significant reduction in the expression of SNARE proteins.<br /><em><strong>Conclusion:</strong></em> Increased expression of SNARE proteins was possibly a compensatory mechanism in response to insulin resistance in the skeletal muscles of diabetic rats. Resveratrol non-significantly reduced the expression of SNARE proteins by enhancing insulin sensitivity, where this effect was dose-dependent. Thus, higher doses of resveratrol and longer intervention periods could probably be more effective. Another molecular mechanism of the anti-diabetic properties of resveratrol was identified with an effect on the expression of SNARE proteins.http://ijbms.mums.ac.ir/article_13988_e93b36c65dab92ba1b7dca92dd73787b.pdfresveratrolsnap-23snare complex proteinssyntaxin-4type 2 diabetesvamp-2 |
| spellingShingle | Azam Rezaei Farimani Mohammad Taghi Goodarzi Massoud Saidijam Reza Yadgar Azari Sadegh Zarei Soheila Asadi Effect of resveratrol on SNARE proteins expression and insulin resistance in skeletal muscle of diabetic rats Iranian Journal of Basic Medical Sciences resveratrol snap-23 snare complex proteins syntaxin-4 type 2 diabetes vamp-2 |
| title | Effect of resveratrol on SNARE proteins expression and insulin resistance in skeletal muscle of diabetic rats |
| title_full | Effect of resveratrol on SNARE proteins expression and insulin resistance in skeletal muscle of diabetic rats |
| title_fullStr | Effect of resveratrol on SNARE proteins expression and insulin resistance in skeletal muscle of diabetic rats |
| title_full_unstemmed | Effect of resveratrol on SNARE proteins expression and insulin resistance in skeletal muscle of diabetic rats |
| title_short | Effect of resveratrol on SNARE proteins expression and insulin resistance in skeletal muscle of diabetic rats |
| title_sort | effect of resveratrol on snare proteins expression and insulin resistance in skeletal muscle of diabetic rats |
| topic | resveratrol snap-23 snare complex proteins syntaxin-4 type 2 diabetes vamp-2 |
| url | http://ijbms.mums.ac.ir/article_13988_e93b36c65dab92ba1b7dca92dd73787b.pdf |
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