The Public health problem and resistant bacteria in lowand middle-income countries
Introduction. Antimicrobial resistance has been declared a major public health problem. As a result of excessive antibiotic usage, it became an environmental issue. This problem is now more visible in Low-and Middle-Income Countries, where it increases the social burden. One of the newest methods to...
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| Format: | Article |
| Language: | English |
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Asociatia de Biosiguranta si Biosecuritate
2024-01-01
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| Series: | One Health & Risk Management |
| Subjects: | |
| Online Access: | https://journal.ohrm.bba.md/index.php/journal-ohrm-bba-md/article/view/570 |
| _version_ | 1797278799859023872 |
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| author | Alina FERDOHLEB Oana-Simina IACONI Greta BALAN Lucia GALBEN Lukasz DZIEWIT Carles M. BORREGO |
| author_facet | Alina FERDOHLEB Oana-Simina IACONI Greta BALAN Lucia GALBEN Lukasz DZIEWIT Carles M. BORREGO |
| author_sort | Alina FERDOHLEB |
| collection | DOAJ |
| description | Introduction. Antimicrobial resistance has been declared a major public health problem. As a result of excessive antibiotic usage, it became an environmental issue. This problem is now more visible in Low-and Middle-Income Countries, where it increases the social burden. One of the newest methods to fight antimicrobial-resistant bacteria (ARB) is by using bacteriophages. Aim Identification and characterization of possible phage targets from waterland. Material and methods The strains were obtained from inpatients and identified using VITEK 2 Compact and culture. The resistance profiles were determined by the disk diffusion method and interpreted according to EUCAST methodology. The presence of resistance mechanisms was checked by phenotypic testing. 31 bacterial strains were selected for research. Results K. pneumoniae, P. aeruginosa, Acinetobacter spp., S. aureus, E. coli and Enterococcus spp. were identified. The resistance profile of the isolates showed: 61,5% of K. pneumoniae isolates were PDR, and 23,1% were susceptible only to Carbapenems. E. coli strains were XDR, 71,4% of P. aeruginosa and 75% of Acinetobacter spp. were PDR bacteria. The susceptibility profile of S. aureus strains showed that 3/4 were resistant to Cephalosporins and Fluoroquinolones. Conclusions. Combating the AMR phenomenon starts with knowing the pathogens present in the environment. This study is the cornerstone for further research that will ultimately lead to finding suitable phages for water treatment in Moldova, hoping they will reduce the economic and social burden and also, environmental contamination. |
| first_indexed | 2024-03-07T16:13:25Z |
| format | Article |
| id | doaj.art-dece8f38661145d9a0f1bee50664bc9e |
| institution | Directory Open Access Journal |
| issn | 2587-3458 2587-3466 |
| language | English |
| last_indexed | 2024-03-07T16:13:25Z |
| publishDate | 2024-01-01 |
| publisher | Asociatia de Biosiguranta si Biosecuritate |
| record_format | Article |
| series | One Health & Risk Management |
| spelling | doaj.art-dece8f38661145d9a0f1bee50664bc9e2024-03-04T16:41:33ZengAsociatia de Biosiguranta si BiosecuritateOne Health & Risk Management2587-34582587-34662024-01-0151344210.38045/ohrm.2024.1.05The Public health problem and resistant bacteria in lowand middle-income countriesAlina FERDOHLEB 0https://orcid.org/0000-0003-1344-5466Oana-Simina IACONI 1https://orcid.org/0009-0003-3139-7004Greta BALAN2https://orcid.org/0000-0003-3704-3584Lucia GALBEN3https://orcid.org/0009-0005-3154-7193Lukasz DZIEWIT4https://orcid.org/0000-0002-5057-2811Carles M. BORREGO5https://orcid.org/0000-0002-2708-3753Nicolae Testemitanu State University of Medicine and Pharmacy, Chisinau, Republic of MoldovaNicolae Testemitanu State University of Medicine and Pharmacy, Chisinau, Republic of MoldovaNicolae Testemitanu State University of Medicine and Pharmacy, Chisinau, Republic of MoldovaTimofei Mosneaga Republican Clinical Hospital, Chisinau, Republic of MoldovaInstitute of Microbiology, Faculty of Biology, University of Warsaw, Warsaw, PolandCatalan Institute for Water Research (ICRA-CERCA), Institute of Aquatic Ecology, University of Gi-rona, Girona, SpainIntroduction. Antimicrobial resistance has been declared a major public health problem. As a result of excessive antibiotic usage, it became an environmental issue. This problem is now more visible in Low-and Middle-Income Countries, where it increases the social burden. One of the newest methods to fight antimicrobial-resistant bacteria (ARB) is by using bacteriophages. Aim Identification and characterization of possible phage targets from waterland. Material and methods The strains were obtained from inpatients and identified using VITEK 2 Compact and culture. The resistance profiles were determined by the disk diffusion method and interpreted according to EUCAST methodology. The presence of resistance mechanisms was checked by phenotypic testing. 31 bacterial strains were selected for research. Results K. pneumoniae, P. aeruginosa, Acinetobacter spp., S. aureus, E. coli and Enterococcus spp. were identified. The resistance profile of the isolates showed: 61,5% of K. pneumoniae isolates were PDR, and 23,1% were susceptible only to Carbapenems. E. coli strains were XDR, 71,4% of P. aeruginosa and 75% of Acinetobacter spp. were PDR bacteria. The susceptibility profile of S. aureus strains showed that 3/4 were resistant to Cephalosporins and Fluoroquinolones. Conclusions. Combating the AMR phenomenon starts with knowing the pathogens present in the environment. This study is the cornerstone for further research that will ultimately lead to finding suitable phages for water treatment in Moldova, hoping they will reduce the economic and social burden and also, environmental contamination.https://journal.ohrm.bba.md/index.php/journal-ohrm-bba-md/article/view/570antimicrobial resistancebacterialmicssusceptibility testingbacteriophageswater treatment |
| spellingShingle | Alina FERDOHLEB Oana-Simina IACONI Greta BALAN Lucia GALBEN Lukasz DZIEWIT Carles M. BORREGO The Public health problem and resistant bacteria in lowand middle-income countries One Health & Risk Management antimicrobial resistance bacteria lmics susceptibility testing bacteriophages water treatment |
| title | The Public health problem and resistant bacteria in lowand middle-income countries |
| title_full | The Public health problem and resistant bacteria in lowand middle-income countries |
| title_fullStr | The Public health problem and resistant bacteria in lowand middle-income countries |
| title_full_unstemmed | The Public health problem and resistant bacteria in lowand middle-income countries |
| title_short | The Public health problem and resistant bacteria in lowand middle-income countries |
| title_sort | public health problem and resistant bacteria in lowand middle income countries |
| topic | antimicrobial resistance bacteria lmics susceptibility testing bacteriophages water treatment |
| url | https://journal.ohrm.bba.md/index.php/journal-ohrm-bba-md/article/view/570 |
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