Advances in Cardiac Development and Regeneration Using Zebrafish as a Model System for High-Throughput Research
Heart disease is the leading cause of death in the United States and worldwide. Understanding the molecular mechanisms of cardiac development and regeneration will improve diagnostic and therapeutic interventions against heart disease. In this direction, zebrafish is an excellent model because sever...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Journal of Developmental Biology |
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| Online Access: | https://www.mdpi.com/2221-3759/9/4/40 |
| _version_ | 1827671892633321472 |
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| author | Nicholas Francoeur Rwik Sen |
| author_facet | Nicholas Francoeur Rwik Sen |
| author_sort | Nicholas Francoeur |
| collection | DOAJ |
| description | Heart disease is the leading cause of death in the United States and worldwide. Understanding the molecular mechanisms of cardiac development and regeneration will improve diagnostic and therapeutic interventions against heart disease. In this direction, zebrafish is an excellent model because several processes of zebrafish heart development are largely conserved in humans, and zebrafish has several advantages as a model organism. Zebrafish transcriptomic profiles undergo alterations during different stages of cardiac development and regeneration which are revealed by RNA-sequencing. ChIP-sequencing has detected genome-wide occupancy of histone post-translational modifications that epigenetically regulate gene expression and identified a locus with enhancer-like characteristics. ATAC-sequencing has identified active enhancers in cardiac progenitor cells during early developmental stages which overlap with occupancy of histone modifications of active transcription as determined by ChIP-sequencing. CRISPR-mediated editing of the zebrafish genome shows how chromatin modifiers and DNA-binding proteins regulate heart development, in association with crucial signaling pathways. Hence, more studies in this direction are essential to improve human health because they answer fundamental questions on cardiac development and regeneration, their differences, and why zebrafish hearts regenerate upon injury, unlike humans. This review focuses on some of the latest studies using state-of-the-art technology enabled by the elegant yet simple zebrafish. |
| first_indexed | 2024-03-10T03:50:10Z |
| format | Article |
| id | doaj.art-ded286179362478491ad8fcf585af596 |
| institution | Directory Open Access Journal |
| issn | 2221-3759 |
| language | English |
| last_indexed | 2024-03-10T03:50:10Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Developmental Biology |
| spelling | doaj.art-ded286179362478491ad8fcf585af5962023-11-23T08:59:30ZengMDPI AGJournal of Developmental Biology2221-37592021-09-01944010.3390/jdb9040040Advances in Cardiac Development and Regeneration Using Zebrafish as a Model System for High-Throughput ResearchNicholas Francoeur0Rwik Sen1Active Motif, Incorporated, 1914 Palomar Oaks Way, Suite 150, Carlsbad, CA 92008, USAActive Motif, Incorporated, 1914 Palomar Oaks Way, Suite 150, Carlsbad, CA 92008, USAHeart disease is the leading cause of death in the United States and worldwide. Understanding the molecular mechanisms of cardiac development and regeneration will improve diagnostic and therapeutic interventions against heart disease. In this direction, zebrafish is an excellent model because several processes of zebrafish heart development are largely conserved in humans, and zebrafish has several advantages as a model organism. Zebrafish transcriptomic profiles undergo alterations during different stages of cardiac development and regeneration which are revealed by RNA-sequencing. ChIP-sequencing has detected genome-wide occupancy of histone post-translational modifications that epigenetically regulate gene expression and identified a locus with enhancer-like characteristics. ATAC-sequencing has identified active enhancers in cardiac progenitor cells during early developmental stages which overlap with occupancy of histone modifications of active transcription as determined by ChIP-sequencing. CRISPR-mediated editing of the zebrafish genome shows how chromatin modifiers and DNA-binding proteins regulate heart development, in association with crucial signaling pathways. Hence, more studies in this direction are essential to improve human health because they answer fundamental questions on cardiac development and regeneration, their differences, and why zebrafish hearts regenerate upon injury, unlike humans. This review focuses on some of the latest studies using state-of-the-art technology enabled by the elegant yet simple zebrafish.https://www.mdpi.com/2221-3759/9/4/40cardiac developmentregenerationepigeneticstransgenic reportertranscriptomeepigenetic |
| spellingShingle | Nicholas Francoeur Rwik Sen Advances in Cardiac Development and Regeneration Using Zebrafish as a Model System for High-Throughput Research Journal of Developmental Biology cardiac development regeneration epigenetics transgenic reporter transcriptome epigenetic |
| title | Advances in Cardiac Development and Regeneration Using Zebrafish as a Model System for High-Throughput Research |
| title_full | Advances in Cardiac Development and Regeneration Using Zebrafish as a Model System for High-Throughput Research |
| title_fullStr | Advances in Cardiac Development and Regeneration Using Zebrafish as a Model System for High-Throughput Research |
| title_full_unstemmed | Advances in Cardiac Development and Regeneration Using Zebrafish as a Model System for High-Throughput Research |
| title_short | Advances in Cardiac Development and Regeneration Using Zebrafish as a Model System for High-Throughput Research |
| title_sort | advances in cardiac development and regeneration using zebrafish as a model system for high throughput research |
| topic | cardiac development regeneration epigenetics transgenic reporter transcriptome epigenetic |
| url | https://www.mdpi.com/2221-3759/9/4/40 |
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