Translocating Mycobacterium ulcerans: An experimental model.
Mycobacterium ulcerans is a non-tuberculous environmental mycobacterium responsible for extensive cutaneous and subcutaneous ulcers in mammals, known as Buruli ulcer in humans. M. ulcerans has seldom been detected in the faeces of mammals and has not been detected in human faeces. Nevertheless, the...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2020-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0230544 |
| _version_ | 1819110559356813312 |
|---|---|
| author | Nassim Hammoudi Mustapha Fellag Muriel Militello Amar Bouam Michel Drancourt |
| author_facet | Nassim Hammoudi Mustapha Fellag Muriel Militello Amar Bouam Michel Drancourt |
| author_sort | Nassim Hammoudi |
| collection | DOAJ |
| description | Mycobacterium ulcerans is a non-tuberculous environmental mycobacterium responsible for extensive cutaneous and subcutaneous ulcers in mammals, known as Buruli ulcer in humans. M. ulcerans has seldom been detected in the faeces of mammals and has not been detected in human faeces. Nevertheless, the detection and isolation of M. ulcerans in animal faeces does not fit with the current epidemiological schemes for the disease. Here, using an experimental model in which rats were fed with 109 colony-forming units of M. ulcerans, we detected M. ulcerans DNA in the faeces of challenged rats for two weeks and along their digestive tract for 10 days. M. ulcerans DNA was further detected in the lymphatic system including in the cervical and axillary lymph nodes and the spleen, but not in any other tissue including healthy and broken skin, 10 days post-challenge. These observations indicate that in some herbivorous mammals, M. ulcerans contamination by the digestive route may precede translocation and limited contamination of the lymphatic tissues without systemic infection. These herbivorous mammals may be sources of M. ulcerans for exposed populations but are unlikely to be reservoirs for the pathogen. |
| first_indexed | 2024-12-22T03:43:39Z |
| format | Article |
| id | doaj.art-ded49484bfa74b9dab713b6550b3d4c5 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T03:43:39Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ded49484bfa74b9dab713b6550b3d4c52022-12-21T18:40:12ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-011512e023054410.1371/journal.pone.0230544Translocating Mycobacterium ulcerans: An experimental model.Nassim HammoudiMustapha FellagMuriel MilitelloAmar BouamMichel DrancourtMycobacterium ulcerans is a non-tuberculous environmental mycobacterium responsible for extensive cutaneous and subcutaneous ulcers in mammals, known as Buruli ulcer in humans. M. ulcerans has seldom been detected in the faeces of mammals and has not been detected in human faeces. Nevertheless, the detection and isolation of M. ulcerans in animal faeces does not fit with the current epidemiological schemes for the disease. Here, using an experimental model in which rats were fed with 109 colony-forming units of M. ulcerans, we detected M. ulcerans DNA in the faeces of challenged rats for two weeks and along their digestive tract for 10 days. M. ulcerans DNA was further detected in the lymphatic system including in the cervical and axillary lymph nodes and the spleen, but not in any other tissue including healthy and broken skin, 10 days post-challenge. These observations indicate that in some herbivorous mammals, M. ulcerans contamination by the digestive route may precede translocation and limited contamination of the lymphatic tissues without systemic infection. These herbivorous mammals may be sources of M. ulcerans for exposed populations but are unlikely to be reservoirs for the pathogen.https://doi.org/10.1371/journal.pone.0230544 |
| spellingShingle | Nassim Hammoudi Mustapha Fellag Muriel Militello Amar Bouam Michel Drancourt Translocating Mycobacterium ulcerans: An experimental model. PLoS ONE |
| title | Translocating Mycobacterium ulcerans: An experimental model. |
| title_full | Translocating Mycobacterium ulcerans: An experimental model. |
| title_fullStr | Translocating Mycobacterium ulcerans: An experimental model. |
| title_full_unstemmed | Translocating Mycobacterium ulcerans: An experimental model. |
| title_short | Translocating Mycobacterium ulcerans: An experimental model. |
| title_sort | translocating mycobacterium ulcerans an experimental model |
| url | https://doi.org/10.1371/journal.pone.0230544 |
| work_keys_str_mv | AT nassimhammoudi translocatingmycobacteriumulceransanexperimentalmodel AT mustaphafellag translocatingmycobacteriumulceransanexperimentalmodel AT murielmilitello translocatingmycobacteriumulceransanexperimentalmodel AT amarbouam translocatingmycobacteriumulceransanexperimentalmodel AT micheldrancourt translocatingmycobacteriumulceransanexperimentalmodel |