Comparative Study on the Presentation Effect of Photochemical Method and Wire Embolization Method for Making Focal Cerebral Infarction Model
Objective To investigate the two different methods of making animal models of focal cerebral infarction by photochemical method and wire embolization method, and to analyze the correlation between infarct volume and behavior of experimental animals due to different modeling methods.MethodsTotally si...
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| Format: | Article |
| Language: | zho |
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Editorial Office of Laboratory Animal and Comparative Medicine
2022-02-01
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| Series: | Shiyan dongwu yu bijiao yixue |
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| Online Access: | https://www.slarc.org.cn/dwyx/CN/10.12300/j.issn.1674-5817.2021.095 |
| _version_ | 1797987022896365568 |
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| author | LOU Huijuan ZHANG Hongshi WANG Yufeng JIA Meng CONG Deyu |
| author_facet | LOU Huijuan ZHANG Hongshi WANG Yufeng JIA Meng CONG Deyu |
| author_sort | LOU Huijuan |
| collection | DOAJ |
| description | Objective To investigate the two different methods of making animal models of focal cerebral infarction by photochemical method and wire embolization method, and to analyze the correlation between infarct volume and behavior of experimental animals due to different modeling methods.MethodsTotally sixty-four male Sprague-Dawley rats of 300-330 g were randomly divided into three groups: normal group, photochemical group and middle cerebral artery occlusion (MCAO) group. The neurological function scores and behavioral tests on the rats were performed 24 hours after surgery. Then brain tissues were removed by anesthesia and execution, and 2,3,5-triphenyltetrazolium chloride (TTC) staining was performed to assess the brain infarct volume in rats.ResultsThere were different volume infarct foci in the brain of SD rats in photochemical group and MCAO group. In MCAO model, most of the hemispheric cortex, striatum, thalamus, hippocampus and subventricular zone were involved in ischemia, and there were good correlations of neurological function scores and balance beam test scores with infarct volumes. In the photochemical model, the infarct foci were in cerebral cortex, and the neurobehavioral abnormalities were well correlated with the cortical infarct location, but the correlation with the infarct volume was relatively poor.ConclusionIn MCAO modeling experiment, the behavioral outcomes closely correlated with infarct volume size, while the photochemical model can produce site-specific lesions and site-specific behavioral changed, and the behavioral outcomes have poor correlation with infarct volume. |
| first_indexed | 2024-04-11T07:42:17Z |
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| institution | Directory Open Access Journal |
| issn | 1674-5817 |
| language | zho |
| last_indexed | 2024-04-11T07:42:17Z |
| publishDate | 2022-02-01 |
| publisher | Editorial Office of Laboratory Animal and Comparative Medicine |
| record_format | Article |
| series | Shiyan dongwu yu bijiao yixue |
| spelling | doaj.art-ded88ece57d94964bac2578f7b1d432c2022-12-22T04:36:30ZzhoEditorial Office of Laboratory Animal and Comparative MedicineShiyan dongwu yu bijiao yixue1674-58172022-02-01421485310.12300/j.issn.1674-5817.2021.0951674-5817（2022）01-0048-06Comparative Study on the Presentation Effect of Photochemical Method and Wire Embolization Method for Making Focal Cerebral Infarction ModelLOU Huijuan0ZHANG Hongshi1WANG Yufeng2JIA Meng3CONG Deyu4College of Acupuncture and Tuina, Changchun University of Traditional Chinese Medicine, Changchun 130117, ChinaSchool of Nursing, Changchun University of Traditional Chinese Medicine, Changchun 130117, ChinaDepartment of Tuina, Affiliated Hospital of Changchun University of Traditional Chinese Medicine, Changchun 130021, ChinaChangchun New Industry Optoelectronics Technology Co., Changchun 130103, ChinaCollege of Acupuncture and Tuina, Changchun University of Traditional Chinese Medicine, Changchun 130117, ChinaObjective To investigate the two different methods of making animal models of focal cerebral infarction by photochemical method and wire embolization method, and to analyze the correlation between infarct volume and behavior of experimental animals due to different modeling methods.MethodsTotally sixty-four male Sprague-Dawley rats of 300-330 g were randomly divided into three groups: normal group, photochemical group and middle cerebral artery occlusion (MCAO) group. The neurological function scores and behavioral tests on the rats were performed 24 hours after surgery. Then brain tissues were removed by anesthesia and execution, and 2,3,5-triphenyltetrazolium chloride (TTC) staining was performed to assess the brain infarct volume in rats.ResultsThere were different volume infarct foci in the brain of SD rats in photochemical group and MCAO group. In MCAO model, most of the hemispheric cortex, striatum, thalamus, hippocampus and subventricular zone were involved in ischemia, and there were good correlations of neurological function scores and balance beam test scores with infarct volumes. In the photochemical model, the infarct foci were in cerebral cortex, and the neurobehavioral abnormalities were well correlated with the cortical infarct location, but the correlation with the infarct volume was relatively poor.ConclusionIn MCAO modeling experiment, the behavioral outcomes closely correlated with infarct volume size, while the photochemical model can produce site-specific lesions and site-specific behavioral changed, and the behavioral outcomes have poor correlation with infarct volume.https://www.slarc.org.cn/dwyx/CN/10.12300/j.issn.1674-5817.2021.095animal model of focal cerebral infarctionwire embolization methodphotochemical methodbehaviorinfarct volume |
| spellingShingle | LOU Huijuan ZHANG Hongshi WANG Yufeng JIA Meng CONG Deyu Comparative Study on the Presentation Effect of Photochemical Method and Wire Embolization Method for Making Focal Cerebral Infarction Model Shiyan dongwu yu bijiao yixue animal model of focal cerebral infarction wire embolization method photochemical method behavior infarct volume |
| title | Comparative Study on the Presentation Effect of Photochemical Method and Wire Embolization Method for Making Focal Cerebral Infarction Model |
| title_full | Comparative Study on the Presentation Effect of Photochemical Method and Wire Embolization Method for Making Focal Cerebral Infarction Model |
| title_fullStr | Comparative Study on the Presentation Effect of Photochemical Method and Wire Embolization Method for Making Focal Cerebral Infarction Model |
| title_full_unstemmed | Comparative Study on the Presentation Effect of Photochemical Method and Wire Embolization Method for Making Focal Cerebral Infarction Model |
| title_short | Comparative Study on the Presentation Effect of Photochemical Method and Wire Embolization Method for Making Focal Cerebral Infarction Model |
| title_sort | comparative study on the presentation effect of photochemical method and wire embolization method for making focal cerebral infarction model |
| topic | animal model of focal cerebral infarction wire embolization method photochemical method behavior infarct volume |
| url | https://www.slarc.org.cn/dwyx/CN/10.12300/j.issn.1674-5817.2021.095 |
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