Enterocin Cross-Resistance Mediated by ABC Transport Systems
In their struggle for life, bacteria frequently produce antagonistic substances against competitors. Antimicrobial peptides produced by bacteria (known as bacteriocins) are active against other bacteria, but harmless to their producer due to an associated immunity gene that prevents self-inhibition....
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MDPI AG
2021-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/7/1411 |
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| author | Claudia Teso-Pérez Manuel Martínez-Bueno Juan Manuel Peralta-Sánchez Eva Valdivia Mercedes Maqueda M. Esther Fárez-Vidal Antonio M. Martín-Platero |
| author_facet | Claudia Teso-Pérez Manuel Martínez-Bueno Juan Manuel Peralta-Sánchez Eva Valdivia Mercedes Maqueda M. Esther Fárez-Vidal Antonio M. Martín-Platero |
| author_sort | Claudia Teso-Pérez |
| collection | DOAJ |
| description | In their struggle for life, bacteria frequently produce antagonistic substances against competitors. Antimicrobial peptides produced by bacteria (known as bacteriocins) are active against other bacteria, but harmless to their producer due to an associated immunity gene that prevents self-inhibition. However, knowledge of cross-resistance between different types of bacteriocin producer remains very limited. The immune function of certain bacteriocins produced by the <i>Enterococcus</i> genus (known as enterocins) is mediated by an ABC transporter. This is the case for enterocin AS-48, a gene cluster that includes two ABC transporter-like systems (Transporter-1 and 2) and an immunity protein. Transporter-2 in this cluster shows a high similarity to the ABC transporter-like system in MR10A and MR10B enterocin gene clusters. The aim of our study was to determine the possible role of this ABC transporter in cross-resistance between these two different types of enterocin. To this end, we designed different mutants (Tn<i>5</i> derivative and deletion mutants) of the <i>as-48</i> gene cluster in <i>Enterococcus faecalis</i> and cloned them into the pAM401 shuttle vector. Antimicrobial activity assays showed that enterocin AS-48 Transporter-2 is responsible for cross-resistance between AS-48 and MR10A/B enterocin producers and allowed identification of the MR10A/B immunity gene system. These findings open the way to the investigation of resistance beyond homologous bacteriocins. |
| first_indexed | 2024-03-09T04:44:23Z |
| format | Article |
| id | doaj.art-dedd4649459148cb836e2a2da0b55a2e |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T04:44:23Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-dedd4649459148cb836e2a2da0b55a2e2023-12-03T13:17:45ZengMDPI AGMicroorganisms2076-26072021-06-0197141110.3390/microorganisms9071411Enterocin Cross-Resistance Mediated by ABC Transport SystemsClaudia Teso-Pérez0Manuel Martínez-Bueno1Juan Manuel Peralta-Sánchez2Eva Valdivia3Mercedes Maqueda4M. Esther Fárez-Vidal5Antonio M. Martín-Platero6Departamento de Microbiología, Universidad de Granada, Avda. Fuentenueva s/n, 18071 Granada, SpainDepartamento de Microbiología, Universidad de Granada, Avda. Fuentenueva s/n, 18071 Granada, SpainDepartamento de Microbiología, Universidad de Granada, Avda. Fuentenueva s/n, 18071 Granada, SpainDepartamento de Microbiología, Universidad de Granada, Avda. Fuentenueva s/n, 18071 Granada, SpainDepartamento de Microbiología, Universidad de Granada, Avda. Fuentenueva s/n, 18071 Granada, SpainDepartamento de Bioquímica y Biología Molecular III e Inmunología, Facultad de Medicina, Universidad de Granada, 18016 Granada, SpainDepartamento de Microbiología, Universidad de Granada, Avda. Fuentenueva s/n, 18071 Granada, SpainIn their struggle for life, bacteria frequently produce antagonistic substances against competitors. Antimicrobial peptides produced by bacteria (known as bacteriocins) are active against other bacteria, but harmless to their producer due to an associated immunity gene that prevents self-inhibition. However, knowledge of cross-resistance between different types of bacteriocin producer remains very limited. The immune function of certain bacteriocins produced by the <i>Enterococcus</i> genus (known as enterocins) is mediated by an ABC transporter. This is the case for enterocin AS-48, a gene cluster that includes two ABC transporter-like systems (Transporter-1 and 2) and an immunity protein. Transporter-2 in this cluster shows a high similarity to the ABC transporter-like system in MR10A and MR10B enterocin gene clusters. The aim of our study was to determine the possible role of this ABC transporter in cross-resistance between these two different types of enterocin. To this end, we designed different mutants (Tn<i>5</i> derivative and deletion mutants) of the <i>as-48</i> gene cluster in <i>Enterococcus faecalis</i> and cloned them into the pAM401 shuttle vector. Antimicrobial activity assays showed that enterocin AS-48 Transporter-2 is responsible for cross-resistance between AS-48 and MR10A/B enterocin producers and allowed identification of the MR10A/B immunity gene system. These findings open the way to the investigation of resistance beyond homologous bacteriocins.https://www.mdpi.com/2076-2607/9/7/1411enterocinABC transporterbacteriocin immunityMR10A/BAS-48 |
| spellingShingle | Claudia Teso-Pérez Manuel Martínez-Bueno Juan Manuel Peralta-Sánchez Eva Valdivia Mercedes Maqueda M. Esther Fárez-Vidal Antonio M. Martín-Platero Enterocin Cross-Resistance Mediated by ABC Transport Systems Microorganisms enterocin ABC transporter bacteriocin immunity MR10A/B AS-48 |
| title | Enterocin Cross-Resistance Mediated by ABC Transport Systems |
| title_full | Enterocin Cross-Resistance Mediated by ABC Transport Systems |
| title_fullStr | Enterocin Cross-Resistance Mediated by ABC Transport Systems |
| title_full_unstemmed | Enterocin Cross-Resistance Mediated by ABC Transport Systems |
| title_short | Enterocin Cross-Resistance Mediated by ABC Transport Systems |
| title_sort | enterocin cross resistance mediated by abc transport systems |
| topic | enterocin ABC transporter bacteriocin immunity MR10A/B AS-48 |
| url | https://www.mdpi.com/2076-2607/9/7/1411 |
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