The gut microbiota diversity of five Orthoptera (Insecta, Polyneoptera) insects determined by DNA metabarcoding
Most orthopteran insects are phytophagous and some are important pests in agriculture and forests. Many intestinal microflora of Orthoptera insects have been reported, primarily from Acridoidea and there have been few reports of other taxa. In this study, we collected 15 individuals representing fiv...
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2023-03-01
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| Series: | Biodiversity Data Journal |
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| Online Access: | https://bdj.pensoft.net/article/98162/download/pdf/ |
| _version_ | 1827987682460958720 |
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| author | Yantong Liu Lina Zhao Zhongying Qiu Hao Yuan |
| author_facet | Yantong Liu Lina Zhao Zhongying Qiu Hao Yuan |
| author_sort | Yantong Liu |
| collection | DOAJ |
| description | Most orthopteran insects are phytophagous and some are important pests in agriculture and forests. Many intestinal microflora of Orthoptera insects have been reported, primarily from Acridoidea and there have been few reports of other taxa. In this study, we collected 15 individuals representing five species (Ruspolia lineosa, Tetrix japonica, Erianthus versicolor, Gryllotalpa orientalis and Teleogryllus emma) belonging to five orthopteran superfamilies (Tettigonioidea, Tetrigoidea, Eumastacoidea, Gryllotalpoidea and Grylloidea) to characterise and compare the gut microbiota with greater taxonomic width by performing sequencing analysis of the 16S rRNA V4 region in gut material. A total of 606,053 high-quality sequences and 3,105 OTUs were acquired from 15 gut samples representing 24 phyla, 48 classes, 69 orders, 133 families and 219 genera. Firmicutes and bacteria were the most abundant phyla, followed by Bacteroidetes, Cyanobacteria, Actinobacteria and Acidobacteria. At the genus level, Serratia, Citrobacter, Wolbachia, Lactobacillus and Parabacteroides were the most predominant genera in R. lineosa, T. japonica, E. versicolor, G. orientalis and T. emma, respectively. Both Principal Coordinates Analysis (PCoA) and heatmap results revealed significant differences in bacterial community composition across species. Additionally, alpha diversity analysis indicated the bacterial richness was significantly different amongst the five species. |
| first_indexed | 2024-04-09T23:51:53Z |
| format | Article |
| id | doaj.art-dede22df7a3648a6864408ce90a11265 |
| institution | Directory Open Access Journal |
| issn | 1314-2828 |
| language | English |
| last_indexed | 2024-04-09T23:51:53Z |
| publishDate | 2023-03-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | Biodiversity Data Journal |
| spelling | doaj.art-dede22df7a3648a6864408ce90a112652023-03-17T09:11:04ZengPensoft PublishersBiodiversity Data Journal1314-28282023-03-011111810.3897/BDJ.11.e9816298162The gut microbiota diversity of five Orthoptera (Insecta, Polyneoptera) insects determined by DNA metabarcodingYantong Liu0Lina Zhao1Zhongying Qiu2Hao Yuan3School of Basic Medical Sciences, Xi’an Medical UniversityCollege of Life Sciences, Shaanxi Normal UniversitySchool of Basic Medical Sciences, Xi’an Medical UniversitySchool of Basic Medical Sciences, Xi’an Medical UniversityMost orthopteran insects are phytophagous and some are important pests in agriculture and forests. Many intestinal microflora of Orthoptera insects have been reported, primarily from Acridoidea and there have been few reports of other taxa. In this study, we collected 15 individuals representing five species (Ruspolia lineosa, Tetrix japonica, Erianthus versicolor, Gryllotalpa orientalis and Teleogryllus emma) belonging to five orthopteran superfamilies (Tettigonioidea, Tetrigoidea, Eumastacoidea, Gryllotalpoidea and Grylloidea) to characterise and compare the gut microbiota with greater taxonomic width by performing sequencing analysis of the 16S rRNA V4 region in gut material. A total of 606,053 high-quality sequences and 3,105 OTUs were acquired from 15 gut samples representing 24 phyla, 48 classes, 69 orders, 133 families and 219 genera. Firmicutes and bacteria were the most abundant phyla, followed by Bacteroidetes, Cyanobacteria, Actinobacteria and Acidobacteria. At the genus level, Serratia, Citrobacter, Wolbachia, Lactobacillus and Parabacteroides were the most predominant genera in R. lineosa, T. japonica, E. versicolor, G. orientalis and T. emma, respectively. Both Principal Coordinates Analysis (PCoA) and heatmap results revealed significant differences in bacterial community composition across species. Additionally, alpha diversity analysis indicated the bacterial richness was significantly different amongst the five species.https://bdj.pensoft.net/article/98162/download/pdf/gut microbiotaDNA metabarcodingOrthopterabio |
| spellingShingle | Yantong Liu Lina Zhao Zhongying Qiu Hao Yuan The gut microbiota diversity of five Orthoptera (Insecta, Polyneoptera) insects determined by DNA metabarcoding Biodiversity Data Journal gut microbiota DNA metabarcoding Orthoptera bio |
| title | The gut microbiota diversity of five Orthoptera (Insecta, Polyneoptera) insects determined by DNA metabarcoding |
| title_full | The gut microbiota diversity of five Orthoptera (Insecta, Polyneoptera) insects determined by DNA metabarcoding |
| title_fullStr | The gut microbiota diversity of five Orthoptera (Insecta, Polyneoptera) insects determined by DNA metabarcoding |
| title_full_unstemmed | The gut microbiota diversity of five Orthoptera (Insecta, Polyneoptera) insects determined by DNA metabarcoding |
| title_short | The gut microbiota diversity of five Orthoptera (Insecta, Polyneoptera) insects determined by DNA metabarcoding |
| title_sort | gut microbiota diversity of five orthoptera insecta polyneoptera insects determined by dna metabarcoding |
| topic | gut microbiota DNA metabarcoding Orthoptera bio |
| url | https://bdj.pensoft.net/article/98162/download/pdf/ |
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