Downregulation of CIP2A inhibits cancer cell proliferation and vascularization in renal clear cell carcinoma
Background: CIP2A has been proved to play a role as an oncogene in various types of malignancies while its functionality in renal clear cell carcinoma has not been investigated. Our study aimed to investigate the role of CIP2A in renal clear cell carcinoma and to explore the possible mechanisms. Met...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Palacký University Olomouc, Faculty of Medicine and Dentistry
2020-06-01
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| Series: | Biomedical Papers |
| Subjects: | |
| Online Access: | https://biomed.papers.upol.cz/artkey/bio-202002-0012_downregulation-of-cip2a-inhibits-cancer-cell-proliferation-and-vascularization-in-renal-clear-cell-carcinoma.php |
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| author | Hui Gao Yuqiao Li Tao Lin Yan Cheng Yunbo Ma |
| author_facet | Hui Gao Yuqiao Li Tao Lin Yan Cheng Yunbo Ma |
| author_sort | Hui Gao |
| collection | DOAJ |
| description | Background: CIP2A has been proved to play a role as an oncogene in various types of malignancies while its functionality in renal clear cell carcinoma has not been investigated. Our study aimed to investigate the role of CIP2A in renal clear cell carcinoma and to explore the possible mechanisms. Methods: A total of 80 patients with renal clear cell carcinoma and 32 healthy people were included in the study. Expression of CIP2A was detected by qRT-PCR. CIP2A silencing renal clear cell carcinoma cell line was established. Its effects on cell proliferation and migration were verified by CCK-8 assay and Transwell cell assay, respectively. The effects of CIP2A overexpression on AKT and VEGF were investigated. Results: CIP2A expression level was increased in tumor tissues compared to adjacent healthy tissues. Serum levels of CIP2A protein were higher in cancer patients than in healthy controls, and serum levels of CIP2A protein were increased with increased stage of primary tumor. Serum CIP2A protein can be used to accurately predict renal clear cell carcinoma and its prognosis. CIP2A siRNA silencing inhibited tumor cell proliferation, and treatment with Akt activator reduced this inhibitory effect. CIP2A siRNA silencing decreased the expression level of VEGF and phosphorylation levels of AKT in renal clear cell carcinoma cells, while AKT activator treatment showed no significant effects on CIP2A expression. Conclusion: Downregulation of CIP2A can inhibit cancer cell proliferation and vascularization in renal clear cell carcinoma through inactivation of the Akt pathway and its downstream VEGF. |
| first_indexed | 2024-12-11T09:05:52Z |
| format | Article |
| id | doaj.art-dede3de54485441496f5f41df202cd22 |
| institution | Directory Open Access Journal |
| issn | 1213-8118 1804-7521 |
| language | English |
| last_indexed | 2024-12-11T09:05:52Z |
| publishDate | 2020-06-01 |
| publisher | Palacký University Olomouc, Faculty of Medicine and Dentistry |
| record_format | Article |
| series | Biomedical Papers |
| spelling | doaj.art-dede3de54485441496f5f41df202cd222022-12-22T01:13:38ZengPalacký University Olomouc, Faculty of Medicine and DentistryBiomedical Papers1213-81181804-75212020-06-01164219620210.5507/bp.2019.031bio-202002-0012Downregulation of CIP2A inhibits cancer cell proliferation and vascularization in renal clear cell carcinomaHui Gao0Yuqiao Li1Tao Lin2Yan Cheng3Yunbo Ma4Department of Urology, Liaocheng People's Hospital, Liaocheng City, Shandong Province, P. R. China, 252000Department of Urology, Liaocheng People's Hospital, Liaocheng City, Shandong Province, P. R. China, 252000Department of Urology, Liaocheng People's Hospital, Liaocheng City, Shandong Province, P. R. China, 252000Department of Urology, Liaocheng People's Hospital, Liaocheng City, Shandong Province, P. R. China, 252000Department of Urology, Liaocheng People's Hospital, Liaocheng City, Shandong Province, P. R. China, 252000Background: CIP2A has been proved to play a role as an oncogene in various types of malignancies while its functionality in renal clear cell carcinoma has not been investigated. Our study aimed to investigate the role of CIP2A in renal clear cell carcinoma and to explore the possible mechanisms. Methods: A total of 80 patients with renal clear cell carcinoma and 32 healthy people were included in the study. Expression of CIP2A was detected by qRT-PCR. CIP2A silencing renal clear cell carcinoma cell line was established. Its effects on cell proliferation and migration were verified by CCK-8 assay and Transwell cell assay, respectively. The effects of CIP2A overexpression on AKT and VEGF were investigated. Results: CIP2A expression level was increased in tumor tissues compared to adjacent healthy tissues. Serum levels of CIP2A protein were higher in cancer patients than in healthy controls, and serum levels of CIP2A protein were increased with increased stage of primary tumor. Serum CIP2A protein can be used to accurately predict renal clear cell carcinoma and its prognosis. CIP2A siRNA silencing inhibited tumor cell proliferation, and treatment with Akt activator reduced this inhibitory effect. CIP2A siRNA silencing decreased the expression level of VEGF and phosphorylation levels of AKT in renal clear cell carcinoma cells, while AKT activator treatment showed no significant effects on CIP2A expression. Conclusion: Downregulation of CIP2A can inhibit cancer cell proliferation and vascularization in renal clear cell carcinoma through inactivation of the Akt pathway and its downstream VEGF.https://biomed.papers.upol.cz/artkey/bio-202002-0012_downregulation-of-cip2a-inhibits-cancer-cell-proliferation-and-vascularization-in-renal-clear-cell-carcinoma.phprenal clear cell carcinomacip2aaktvegf |
| spellingShingle | Hui Gao Yuqiao Li Tao Lin Yan Cheng Yunbo Ma Downregulation of CIP2A inhibits cancer cell proliferation and vascularization in renal clear cell carcinoma Biomedical Papers renal clear cell carcinoma cip2a akt vegf |
| title | Downregulation of CIP2A inhibits cancer cell proliferation and vascularization in renal clear cell carcinoma |
| title_full | Downregulation of CIP2A inhibits cancer cell proliferation and vascularization in renal clear cell carcinoma |
| title_fullStr | Downregulation of CIP2A inhibits cancer cell proliferation and vascularization in renal clear cell carcinoma |
| title_full_unstemmed | Downregulation of CIP2A inhibits cancer cell proliferation and vascularization in renal clear cell carcinoma |
| title_short | Downregulation of CIP2A inhibits cancer cell proliferation and vascularization in renal clear cell carcinoma |
| title_sort | downregulation of cip2a inhibits cancer cell proliferation and vascularization in renal clear cell carcinoma |
| topic | renal clear cell carcinoma cip2a akt vegf |
| url | https://biomed.papers.upol.cz/artkey/bio-202002-0012_downregulation-of-cip2a-inhibits-cancer-cell-proliferation-and-vascularization-in-renal-clear-cell-carcinoma.php |
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