Wogonin inhibits in vitro herpes simplex virus type 1 and 2 infection by modulating cellular NF-κB and MAPK pathways
Abstract Background Wogonin, a natural flavonoid-like chemical compound, exhibits anti-inflammatory, antitumor, antiviral, neuroprotective, and anxiolytic effects by modulating a variety of cellular signaling pathways including PI3K-Akt, p53, nuclear factor κB (NF-κB), mitogen-activated protein kina...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-07-01
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| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12866-020-01916-2 |
| _version_ | 1811331553880440832 |
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| author | Ying Chu Xiaowen Lv Longfeng Zhang Xingli Fu Siwei Song Airong Su Deyan Chen Lianhong Xu Yongfang Wang Zhiwei Wu Zhihua Yun |
| author_facet | Ying Chu Xiaowen Lv Longfeng Zhang Xingli Fu Siwei Song Airong Su Deyan Chen Lianhong Xu Yongfang Wang Zhiwei Wu Zhihua Yun |
| author_sort | Ying Chu |
| collection | DOAJ |
| description | Abstract Background Wogonin, a natural flavonoid-like chemical compound, exhibits anti-inflammatory, antitumor, antiviral, neuroprotective, and anxiolytic effects by modulating a variety of cellular signaling pathways including PI3K-Akt, p53, nuclear factor κB (NF-κB), mitogen-activated protein kinase (MAPK) pathways. In this study, its antiviral effect against herpes simplex virus (HSV) type 1 and 2 (HSV-1 and HSV-2) replication was investigated. Results Wogonin suppressed HSV-2-induced cytopathic effect (CPE) and reduced viral mRNA transcription, viral protein synthesis, and infectious virion particle titers in a dose-dependent manner. A time-of-drug-addition assay demonstrated that wogonin acted as a postentry viral inhibitor. Wogonin also significantly reduced HSV-induced NF-κB and MAPK pathway activation, which has previously been demonstrated to be important for viral replication. Conclusions Our results suggest that the anti-herpes effect of wogonin may be mediated by modulation of cellular NF-κB and JNK/p38 MAPK pathways and imply that wogonin may be useful as an anti-HSV agent. |
| first_indexed | 2024-04-13T16:22:23Z |
| format | Article |
| id | doaj.art-dee3cbae6ba64fd8860224e1da70b6b2 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-04-13T16:22:23Z |
| publishDate | 2020-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-dee3cbae6ba64fd8860224e1da70b6b22022-12-22T02:39:51ZengBMCBMC Microbiology1471-21802020-07-0120111110.1186/s12866-020-01916-2Wogonin inhibits in vitro herpes simplex virus type 1 and 2 infection by modulating cellular NF-κB and MAPK pathwaysYing Chu0Xiaowen Lv1Longfeng Zhang2Xingli Fu3Siwei Song4Airong Su5Deyan Chen6Lianhong Xu7Yongfang Wang8Zhiwei Wu9Zhihua Yun10Clinical Laboratory, Wujin Hospital Affiliated with Jiangsu University, Wujin Clinical College of Xuzhou Medical UniversityDepartment of Pediatrics, Affiliated Hangzhou First People’s Hospital, Zhejiang University School of MedicineClinical Laboratory, Affiliated Hospital of Jiangsu UniversityHealth Science Center, Jiangsu UniversityDepartment of Cardiology, Renmin Hospital of Wuhan UniversityCentral Laboratory, The Second Affiliated Hospital of Nanjing Medical UniversityCenter for Public Health Research, Medical School, Nanjing UniversityClinical Laboratory, Wujin Hospital Affiliated with Jiangsu University, Wujin Clinical College of Xuzhou Medical UniversityClinical Laboratory, Wujin Hospital Affiliated with Jiangsu University, Wujin Clinical College of Xuzhou Medical UniversityCenter for Public Health Research, Medical School, Nanjing UniversityClinical Laboratory, Wujin Hospital Affiliated with Jiangsu University, Wujin Clinical College of Xuzhou Medical UniversityAbstract Background Wogonin, a natural flavonoid-like chemical compound, exhibits anti-inflammatory, antitumor, antiviral, neuroprotective, and anxiolytic effects by modulating a variety of cellular signaling pathways including PI3K-Akt, p53, nuclear factor κB (NF-κB), mitogen-activated protein kinase (MAPK) pathways. In this study, its antiviral effect against herpes simplex virus (HSV) type 1 and 2 (HSV-1 and HSV-2) replication was investigated. Results Wogonin suppressed HSV-2-induced cytopathic effect (CPE) and reduced viral mRNA transcription, viral protein synthesis, and infectious virion particle titers in a dose-dependent manner. A time-of-drug-addition assay demonstrated that wogonin acted as a postentry viral inhibitor. Wogonin also significantly reduced HSV-induced NF-κB and MAPK pathway activation, which has previously been demonstrated to be important for viral replication. Conclusions Our results suggest that the anti-herpes effect of wogonin may be mediated by modulation of cellular NF-κB and JNK/p38 MAPK pathways and imply that wogonin may be useful as an anti-HSV agent.http://link.springer.com/article/10.1186/s12866-020-01916-2WogoninHerpes simplex virus (HSV)Antiviral activityNuclear factor κB (NF-κB)Mitogen-activated protein kinase (MAPK) |
| spellingShingle | Ying Chu Xiaowen Lv Longfeng Zhang Xingli Fu Siwei Song Airong Su Deyan Chen Lianhong Xu Yongfang Wang Zhiwei Wu Zhihua Yun Wogonin inhibits in vitro herpes simplex virus type 1 and 2 infection by modulating cellular NF-κB and MAPK pathways BMC Microbiology Wogonin Herpes simplex virus (HSV) Antiviral activity Nuclear factor κB (NF-κB) Mitogen-activated protein kinase (MAPK) |
| title | Wogonin inhibits in vitro herpes simplex virus type 1 and 2 infection by modulating cellular NF-κB and MAPK pathways |
| title_full | Wogonin inhibits in vitro herpes simplex virus type 1 and 2 infection by modulating cellular NF-κB and MAPK pathways |
| title_fullStr | Wogonin inhibits in vitro herpes simplex virus type 1 and 2 infection by modulating cellular NF-κB and MAPK pathways |
| title_full_unstemmed | Wogonin inhibits in vitro herpes simplex virus type 1 and 2 infection by modulating cellular NF-κB and MAPK pathways |
| title_short | Wogonin inhibits in vitro herpes simplex virus type 1 and 2 infection by modulating cellular NF-κB and MAPK pathways |
| title_sort | wogonin inhibits in vitro herpes simplex virus type 1 and 2 infection by modulating cellular nf κb and mapk pathways |
| topic | Wogonin Herpes simplex virus (HSV) Antiviral activity Nuclear factor κB (NF-κB) Mitogen-activated protein kinase (MAPK) |
| url | http://link.springer.com/article/10.1186/s12866-020-01916-2 |
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