Estimation of metabolic fluxes distribution in Saccharomyces cerevisiae during the production of volatile compounds of Tequila
A stoichiometric model for Saccharomyces cerevisiae is reconstructed to analyze the continuous fermentation process of agave juice in Tequila production. The metabolic model contains 94 metabolites and 117 biochemical reactions. From the above set of reactions, 93 of them are linked to internal bioc...
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AIMS Press
2021-07-01
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| Series: | Mathematical Biosciences and Engineering |
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| Online Access: | https://www.aimspress.com/article/doi/10.3934/mbe.2021259?viewType=HTML |
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| author | José Daniel Padilla-de la-Rosa Mario Alberto García-Ramírez Anne Christine Gschaedler-Mathis Abril Ivette Gómez-Guzmán Josué R. Solís-Pacheco Orfil González-Reynoso |
| author_facet | José Daniel Padilla-de la-Rosa Mario Alberto García-Ramírez Anne Christine Gschaedler-Mathis Abril Ivette Gómez-Guzmán Josué R. Solís-Pacheco Orfil González-Reynoso |
| author_sort | José Daniel Padilla-de la-Rosa |
| collection | DOAJ |
| description | A stoichiometric model for Saccharomyces cerevisiae is reconstructed to analyze the continuous fermentation process of agave juice in Tequila production. The metabolic model contains 94 metabolites and 117 biochemical reactions. From the above set of reactions, 93 of them are linked to internal biochemical reactions and 24 are related to transport fluxes between the medium and the cell. The central metabolism of S. cerevisiae includes the synthesis for 20 amino-acids, carbohydrates, lipids, DNA and RNA. Using flux balance analysis (FBA), different physiological states of S. cerevisiae are shown during the fermentative process; these states are compared with experimental data under different dilution rates (0.04-0.12 h$ ^{-1} $). Moreover, the model performs anabolic and catabolic biochemical reactions for the production of higher alcohols. The importance of the Saccharomyces cerevisiae genomic model in the area of alcoholic beverage fermentation is due to the fact that it allows to estimate the metabolic fluxes during the beverage fermentation process and a physiology state of the microorganism. |
| first_indexed | 2024-12-20T18:17:18Z |
| format | Article |
| id | doaj.art-dee537d1529a4ac3aa0b93d6760c95ca |
| institution | Directory Open Access Journal |
| issn | 1551-0018 |
| language | English |
| last_indexed | 2024-12-20T18:17:18Z |
| publishDate | 2021-07-01 |
| publisher | AIMS Press |
| record_format | Article |
| series | Mathematical Biosciences and Engineering |
| spelling | doaj.art-dee537d1529a4ac3aa0b93d6760c95ca2022-12-21T19:30:21ZengAIMS PressMathematical Biosciences and Engineering1551-00182021-07-011855094511310.3934/mbe.2021259Estimation of metabolic fluxes distribution in Saccharomyces cerevisiae during the production of volatile compounds of TequilaJosé Daniel Padilla-de la-Rosa0Mario Alberto García-Ramírez1Anne Christine Gschaedler-Mathis2Abril Ivette Gómez-Guzmán3 Josué R. Solís-Pacheco4Orfil González-Reynoso51. Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, Zapopan, C.P. 44270 Jalisco, México2. Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Blvd. M. García Barragán # 1451, C.P. 44430, Guadalajara, Jalisco, México1. Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, Zapopan, C.P. 44270 Jalisco, México3. Universidad Del Valle de México, Campus Zapopan, C.P. 45010, Jalisco, Mexico2. Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Blvd. M. García Barragán # 1451, C.P. 44430, Guadalajara, Jalisco, México2. Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Blvd. M. García Barragán # 1451, C.P. 44430, Guadalajara, Jalisco, MéxicoA stoichiometric model for Saccharomyces cerevisiae is reconstructed to analyze the continuous fermentation process of agave juice in Tequila production. The metabolic model contains 94 metabolites and 117 biochemical reactions. From the above set of reactions, 93 of them are linked to internal biochemical reactions and 24 are related to transport fluxes between the medium and the cell. The central metabolism of S. cerevisiae includes the synthesis for 20 amino-acids, carbohydrates, lipids, DNA and RNA. Using flux balance analysis (FBA), different physiological states of S. cerevisiae are shown during the fermentative process; these states are compared with experimental data under different dilution rates (0.04-0.12 h$ ^{-1} $). Moreover, the model performs anabolic and catabolic biochemical reactions for the production of higher alcohols. The importance of the Saccharomyces cerevisiae genomic model in the area of alcoholic beverage fermentation is due to the fact that it allows to estimate the metabolic fluxes during the beverage fermentation process and a physiology state of the microorganism.https://www.aimspress.com/article/doi/10.3934/mbe.2021259?viewType=HTMLflux balance analysissaccharomyces cerevisiaetequilafermentationhigher alcohols |
| spellingShingle | José Daniel Padilla-de la-Rosa Mario Alberto García-Ramírez Anne Christine Gschaedler-Mathis Abril Ivette Gómez-Guzmán Josué R. Solís-Pacheco Orfil González-Reynoso Estimation of metabolic fluxes distribution in Saccharomyces cerevisiae during the production of volatile compounds of Tequila Mathematical Biosciences and Engineering flux balance analysis saccharomyces cerevisiae tequila fermentation higher alcohols |
| title | Estimation of metabolic fluxes distribution in Saccharomyces cerevisiae during the production of volatile compounds of Tequila |
| title_full | Estimation of metabolic fluxes distribution in Saccharomyces cerevisiae during the production of volatile compounds of Tequila |
| title_fullStr | Estimation of metabolic fluxes distribution in Saccharomyces cerevisiae during the production of volatile compounds of Tequila |
| title_full_unstemmed | Estimation of metabolic fluxes distribution in Saccharomyces cerevisiae during the production of volatile compounds of Tequila |
| title_short | Estimation of metabolic fluxes distribution in Saccharomyces cerevisiae during the production of volatile compounds of Tequila |
| title_sort | estimation of metabolic fluxes distribution in saccharomyces cerevisiae during the production of volatile compounds of tequila |
| topic | flux balance analysis saccharomyces cerevisiae tequila fermentation higher alcohols |
| url | https://www.aimspress.com/article/doi/10.3934/mbe.2021259?viewType=HTML |
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