Injection of a PMMA‐doped MSC spheroid gel for the treatment of painful osteoporotic vertebral compression fractures
Abstract We aimed to develop a biocompatible treatment to overcome the limitations of polymethyl methacrylate (PMMA) vertebroplasty for osteoporotic compression fracture patients. We synthesized an injectable hydrogel containing PMMA. Mesenchymal stem cell (MSC) spheroids were included in the inject...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-11-01
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| Series: | Bioengineering & Translational Medicine |
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| Online Access: | https://doi.org/10.1002/btm2.10577 |
| _version_ | 1797556886284795904 |
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| author | Wan‐Kyu Ko Daye Lee Seong Jun Kim Gong Ho Han Donghyun Lee Seung Hun Sheen Seil Sohn |
| author_facet | Wan‐Kyu Ko Daye Lee Seong Jun Kim Gong Ho Han Donghyun Lee Seung Hun Sheen Seil Sohn |
| author_sort | Wan‐Kyu Ko |
| collection | DOAJ |
| description | Abstract We aimed to develop a biocompatible treatment to overcome the limitations of polymethyl methacrylate (PMMA) vertebroplasty for osteoporotic compression fracture patients. We synthesized an injectable hydrogel containing PMMA. Mesenchymal stem cell (MSC) spheroids were included in the injectable PMMA‐doped gel (= PMMA‐doped spheroid gel). In vitro, the osteogenic/anti‐inflammatory effects of the embedded spheroids were investigated by the quantitative real‐time polymerase chain reaction method. In vivo, we used ovariectomy (OVX)‐induced osteoporotic rats with injured femurs to investigate the pain‐relief effects. The OVX rats were divided into four groups according to the materials injected (non, PMMA, PMMA gel, and PMMA‐spheroid gel) into the lesion. The immunofluorescence (IF) intensity levels of painful markers in dorsal root ganglia (DRG) were measured. In vitro, a volumetric ratio of the gel of 8 (gel):2 (PMMA) was non‐cytotoxic for MSCs and promoted the expression of osteogenic/anti‐inflammatory markers. In vivo, the values of several bone parameters in the PMMA‐doped spheroid gel group showed remarkable increases compared to those in the PMMA group. In addition, the IF intensity levels of the painful markers were noticeably decreased in the PMMA‐spheroid gel group. We, therefore, suggest that this treatment can be useful for osteoporotic vertebral compression fracture patients. |
| first_indexed | 2024-03-10T17:09:24Z |
| format | Article |
| id | doaj.art-dee59de5e78744429bba687ea88c3320 |
| institution | Directory Open Access Journal |
| issn | 2380-6761 |
| language | English |
| last_indexed | 2024-03-10T17:09:24Z |
| publishDate | 2023-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioengineering & Translational Medicine |
| spelling | doaj.art-dee59de5e78744429bba687ea88c33202023-11-20T10:44:12ZengWileyBioengineering & Translational Medicine2380-67612023-11-0186n/an/a10.1002/btm2.10577Injection of a PMMA‐doped MSC spheroid gel for the treatment of painful osteoporotic vertebral compression fracturesWan‐Kyu Ko0Daye Lee1Seong Jun Kim2Gong Ho Han3Donghyun Lee4Seung Hun Sheen5Seil Sohn6Department of Neurosurgery, CHA Bundang Medical Center CHA University Seongnam‐si Gyeonggi‐do Republic of KoreaDepartment of Neurosurgery, CHA Bundang Medical Center CHA University Seongnam‐si Gyeonggi‐do Republic of KoreaDepartment of Neurosurgery, CHA Bundang Medical Center CHA University Seongnam‐si Gyeonggi‐do Republic of KoreaDepartment of Neurosurgery, CHA Bundang Medical Center CHA University Seongnam‐si Gyeonggi‐do Republic of KoreaPreclinical Research Center Daegu‐Gyeongbuk Medical Innovation Foundation (DGMIF) Daegu Republic of KoreaDepartment of Neurosurgery, CHA Bundang Medical Center CHA University Seongnam‐si Gyeonggi‐do Republic of KoreaDepartment of Neurosurgery, CHA Bundang Medical Center CHA University Seongnam‐si Gyeonggi‐do Republic of KoreaAbstract We aimed to develop a biocompatible treatment to overcome the limitations of polymethyl methacrylate (PMMA) vertebroplasty for osteoporotic compression fracture patients. We synthesized an injectable hydrogel containing PMMA. Mesenchymal stem cell (MSC) spheroids were included in the injectable PMMA‐doped gel (= PMMA‐doped spheroid gel). In vitro, the osteogenic/anti‐inflammatory effects of the embedded spheroids were investigated by the quantitative real‐time polymerase chain reaction method. In vivo, we used ovariectomy (OVX)‐induced osteoporotic rats with injured femurs to investigate the pain‐relief effects. The OVX rats were divided into four groups according to the materials injected (non, PMMA, PMMA gel, and PMMA‐spheroid gel) into the lesion. The immunofluorescence (IF) intensity levels of painful markers in dorsal root ganglia (DRG) were measured. In vitro, a volumetric ratio of the gel of 8 (gel):2 (PMMA) was non‐cytotoxic for MSCs and promoted the expression of osteogenic/anti‐inflammatory markers. In vivo, the values of several bone parameters in the PMMA‐doped spheroid gel group showed remarkable increases compared to those in the PMMA group. In addition, the IF intensity levels of the painful markers were noticeably decreased in the PMMA‐spheroid gel group. We, therefore, suggest that this treatment can be useful for osteoporotic vertebral compression fracture patients.https://doi.org/10.1002/btm2.10577mesenchymal stem cellosteoporotic fracturepainPMMAspheroid |
| spellingShingle | Wan‐Kyu Ko Daye Lee Seong Jun Kim Gong Ho Han Donghyun Lee Seung Hun Sheen Seil Sohn Injection of a PMMA‐doped MSC spheroid gel for the treatment of painful osteoporotic vertebral compression fractures Bioengineering & Translational Medicine mesenchymal stem cell osteoporotic fracture pain PMMA spheroid |
| title | Injection of a PMMA‐doped MSC spheroid gel for the treatment of painful osteoporotic vertebral compression fractures |
| title_full | Injection of a PMMA‐doped MSC spheroid gel for the treatment of painful osteoporotic vertebral compression fractures |
| title_fullStr | Injection of a PMMA‐doped MSC spheroid gel for the treatment of painful osteoporotic vertebral compression fractures |
| title_full_unstemmed | Injection of a PMMA‐doped MSC spheroid gel for the treatment of painful osteoporotic vertebral compression fractures |
| title_short | Injection of a PMMA‐doped MSC spheroid gel for the treatment of painful osteoporotic vertebral compression fractures |
| title_sort | injection of a pmma doped msc spheroid gel for the treatment of painful osteoporotic vertebral compression fractures |
| topic | mesenchymal stem cell osteoporotic fracture pain PMMA spheroid |
| url | https://doi.org/10.1002/btm2.10577 |
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