<i>Elaeagnus angustifolia</i> Plant Extract Inhibits Epithelial-Mesenchymal Transition and Induces Apoptosis via HER2 Inactivation and JNK Pathway in HER2-Positive Breast Cancer Cells
<i>Elaeagnus angustifolia</i> (<i>EA</i>) is a medicinal plant used for treating several human diseases in the Middle East. Meanwhile, the outcome of <i>EA</i> extract on HER2-positive breast cancer remains nascent. Thus, we herein investigated the effects of the...
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MDPI AG
2020-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/18/4240 |
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| author | Ayesha Jabeen Anju Sharma Ishita Gupta Hadeel Kheraldine Semir Vranic Ala-Eddin Al Moustafa Halema F. Al Farsi |
| author_facet | Ayesha Jabeen Anju Sharma Ishita Gupta Hadeel Kheraldine Semir Vranic Ala-Eddin Al Moustafa Halema F. Al Farsi |
| author_sort | Ayesha Jabeen |
| collection | DOAJ |
| description | <i>Elaeagnus angustifolia</i> (<i>EA</i>) is a medicinal plant used for treating several human diseases in the Middle East. Meanwhile, the outcome of <i>EA</i> extract on HER2-positive breast cancer remains nascent. Thus, we herein investigated the effects of the aqueous <i>EA</i> extract obtained from the flowers of <i>EA</i> on two HER2-positive breast cancer cell lines, SKBR3 and ZR75-1. Our data revealed that <i>EA</i> extract inhibits cell proliferation and deregulates cell-cycle progression of these two cancer cell lines. <i>EA</i> extract also prevents the progression of epithelial-mesenchymal transition (EMT), an important event for cancer invasion and metastasis; this is accompanied by upregulations of E-cadherin and β-catenin, in addition to downregulations of vimentin and fascin, which are major markers of EMT. Thus, <i>EA</i> extract causes a drastic decrease in cell invasion ability of SKBR3 and ZR75-1 cancer cells. Additionally, we found that <i>EA</i> extract inhibits colony formation of both cell lines in comparison with their matched control. The molecular pathway analysis of HER2 and JNK1/2/3 of <i>EA</i> extract exposed cells revealed that it can block HER2 and JNK1/2/3 activities, which could be the major molecular pathway behind these events. Our findings implicate that <i>EA</i> extract may possess chemo-preventive effects against HER2-positive breast cancer via HER2 inactivation and specifically JNK1/2/3 signaling pathways. |
| first_indexed | 2024-03-10T16:19:03Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T16:19:03Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-dee77debf13d48fa832846f32b8fbb482023-11-20T13:51:52ZengMDPI AGMolecules1420-30492020-09-012518424010.3390/molecules25184240<i>Elaeagnus angustifolia</i> Plant Extract Inhibits Epithelial-Mesenchymal Transition and Induces Apoptosis via HER2 Inactivation and JNK Pathway in HER2-Positive Breast Cancer CellsAyesha Jabeen0Anju Sharma1Ishita Gupta2Hadeel Kheraldine3Semir Vranic4Ala-Eddin Al Moustafa5Halema F. Al Farsi6College of Medicine, QU Health, Qatar University, Doha P.O. Box 2713, QatarCollege of Medicine, QU Health, Qatar University, Doha P.O. Box 2713, QatarCollege of Medicine, QU Health, Qatar University, Doha P.O. Box 2713, QatarCollege of Medicine, QU Health, Qatar University, Doha P.O. Box 2713, QatarCollege of Medicine, QU Health, Qatar University, Doha P.O. Box 2713, QatarCollege of Medicine, QU Health, Qatar University, Doha P.O. Box 2713, QatarCollege of Medicine, QU Health, Qatar University, Doha P.O. Box 2713, Qatar<i>Elaeagnus angustifolia</i> (<i>EA</i>) is a medicinal plant used for treating several human diseases in the Middle East. Meanwhile, the outcome of <i>EA</i> extract on HER2-positive breast cancer remains nascent. Thus, we herein investigated the effects of the aqueous <i>EA</i> extract obtained from the flowers of <i>EA</i> on two HER2-positive breast cancer cell lines, SKBR3 and ZR75-1. Our data revealed that <i>EA</i> extract inhibits cell proliferation and deregulates cell-cycle progression of these two cancer cell lines. <i>EA</i> extract also prevents the progression of epithelial-mesenchymal transition (EMT), an important event for cancer invasion and metastasis; this is accompanied by upregulations of E-cadherin and β-catenin, in addition to downregulations of vimentin and fascin, which are major markers of EMT. Thus, <i>EA</i> extract causes a drastic decrease in cell invasion ability of SKBR3 and ZR75-1 cancer cells. Additionally, we found that <i>EA</i> extract inhibits colony formation of both cell lines in comparison with their matched control. The molecular pathway analysis of HER2 and JNK1/2/3 of <i>EA</i> extract exposed cells revealed that it can block HER2 and JNK1/2/3 activities, which could be the major molecular pathway behind these events. Our findings implicate that <i>EA</i> extract may possess chemo-preventive effects against HER2-positive breast cancer via HER2 inactivation and specifically JNK1/2/3 signaling pathways.https://www.mdpi.com/1420-3049/25/18/4240<i>Elaeagnus angustifolia</i>breast cancerEMTchemopreventionapoptosis |
| spellingShingle | Ayesha Jabeen Anju Sharma Ishita Gupta Hadeel Kheraldine Semir Vranic Ala-Eddin Al Moustafa Halema F. Al Farsi <i>Elaeagnus angustifolia</i> Plant Extract Inhibits Epithelial-Mesenchymal Transition and Induces Apoptosis via HER2 Inactivation and JNK Pathway in HER2-Positive Breast Cancer Cells Molecules <i>Elaeagnus angustifolia</i> breast cancer EMT chemoprevention apoptosis |
| title | <i>Elaeagnus angustifolia</i> Plant Extract Inhibits Epithelial-Mesenchymal Transition and Induces Apoptosis via HER2 Inactivation and JNK Pathway in HER2-Positive Breast Cancer Cells |
| title_full | <i>Elaeagnus angustifolia</i> Plant Extract Inhibits Epithelial-Mesenchymal Transition and Induces Apoptosis via HER2 Inactivation and JNK Pathway in HER2-Positive Breast Cancer Cells |
| title_fullStr | <i>Elaeagnus angustifolia</i> Plant Extract Inhibits Epithelial-Mesenchymal Transition and Induces Apoptosis via HER2 Inactivation and JNK Pathway in HER2-Positive Breast Cancer Cells |
| title_full_unstemmed | <i>Elaeagnus angustifolia</i> Plant Extract Inhibits Epithelial-Mesenchymal Transition and Induces Apoptosis via HER2 Inactivation and JNK Pathway in HER2-Positive Breast Cancer Cells |
| title_short | <i>Elaeagnus angustifolia</i> Plant Extract Inhibits Epithelial-Mesenchymal Transition and Induces Apoptosis via HER2 Inactivation and JNK Pathway in HER2-Positive Breast Cancer Cells |
| title_sort | i elaeagnus angustifolia i plant extract inhibits epithelial mesenchymal transition and induces apoptosis via her2 inactivation and jnk pathway in her2 positive breast cancer cells |
| topic | <i>Elaeagnus angustifolia</i> breast cancer EMT chemoprevention apoptosis |
| url | https://www.mdpi.com/1420-3049/25/18/4240 |
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