A Novel Pectic Polysaccharide of Jujube Pomace: Structural Analysis and Intracellular Antioxidant Activities
After extraction from jujube pomace and purification by two columns (DEAE-Sepharose Fast Flow and Sepharcyl S-300), the structure of SAZMP4 was investigated by HPGPC, GC, FI-IR, GC-MS, NMR, SEM, and AFM. Analysis determined that SAZMP4 (Mw = 28.94 kDa) was a pectic polysaccharide mainly containing 1...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-02-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/9/2/127 |
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| author | Ximeng Lin Keshan Liu Sheng Yin Yimin Qin Peili Shen Qiang Peng |
| author_facet | Ximeng Lin Keshan Liu Sheng Yin Yimin Qin Peili Shen Qiang Peng |
| author_sort | Ximeng Lin |
| collection | DOAJ |
| description | After extraction from jujube pomace and purification by two columns (DEAE-Sepharose Fast Flow and Sepharcyl S-300), the structure of SAZMP4 was investigated by HPGPC, GC, FI-IR, GC-MS, NMR, SEM, and AFM. Analysis determined that SAZMP4 (Mw = 28.94 kDa) was a pectic polysaccharide mainly containing 1,4-linked GalA (93.48%) with side chains of 1,2,4-linked Rha and 1,3,5-linked Ara and terminals of 1-linked Rha and 1-linked Ara, which might be the homogalacturonan (HG) type with side chains of the RG-I type, corresponding to the results of NMR. In AFM and SEM images, self-assembly and aggregation of SAZMP4 were respectively observed indicating its structural features. The antioxidant activity of SAZMP4 against H<sub>2</sub>O<sub>2</sub>-induced oxidative stress in Caco-2 cells was determined by activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) as well as malondialdehyde (MDA) and reactive oxygen species (ROS) levels, indicating SAZMP4 can be a natural antioxidant. Also, a better water retention capacity and thermal stability of SAZMP4 was observed based on DSC analysis, which could be applied in food industry as an additive. |
| first_indexed | 2024-03-12T06:42:44Z |
| format | Article |
| id | doaj.art-dee7ce9381e449db88a644120c92b61d |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-12T06:42:44Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-dee7ce9381e449db88a644120c92b61d2023-09-03T00:56:47ZengMDPI AGAntioxidants2076-39212020-02-019212710.3390/antiox9020127antiox9020127A Novel Pectic Polysaccharide of Jujube Pomace: Structural Analysis and Intracellular Antioxidant ActivitiesXimeng Lin0Keshan Liu1Sheng Yin2Yimin Qin3Peili Shen4Qiang Peng5College of Food Science and Engineering, Northwest A&F University, Yangling 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling 712100, ChinaBeijing Engineering and Technology Research Center of Food Additives, Beijing Technology & Business University (BTBU), Beijing 100048, ChinaState Key Laboratory of Bioactive Seaweed Substances, Ministry of Agriculture Key Laboratory of Seaweed Fertilizers, Qingdao Brightmoon Seaweed Group Co Ltd., Qingdao 266400, ChinaState Key Laboratory of Bioactive Seaweed Substances, Ministry of Agriculture Key Laboratory of Seaweed Fertilizers, Qingdao Brightmoon Seaweed Group Co Ltd., Qingdao 266400, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling 712100, ChinaAfter extraction from jujube pomace and purification by two columns (DEAE-Sepharose Fast Flow and Sepharcyl S-300), the structure of SAZMP4 was investigated by HPGPC, GC, FI-IR, GC-MS, NMR, SEM, and AFM. Analysis determined that SAZMP4 (Mw = 28.94 kDa) was a pectic polysaccharide mainly containing 1,4-linked GalA (93.48%) with side chains of 1,2,4-linked Rha and 1,3,5-linked Ara and terminals of 1-linked Rha and 1-linked Ara, which might be the homogalacturonan (HG) type with side chains of the RG-I type, corresponding to the results of NMR. In AFM and SEM images, self-assembly and aggregation of SAZMP4 were respectively observed indicating its structural features. The antioxidant activity of SAZMP4 against H<sub>2</sub>O<sub>2</sub>-induced oxidative stress in Caco-2 cells was determined by activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) as well as malondialdehyde (MDA) and reactive oxygen species (ROS) levels, indicating SAZMP4 can be a natural antioxidant. Also, a better water retention capacity and thermal stability of SAZMP4 was observed based on DSC analysis, which could be applied in food industry as an additive.https://www.mdpi.com/2076-3921/9/2/127polysaccharidejujube pomacestructural analysisantioxidant activity |
| spellingShingle | Ximeng Lin Keshan Liu Sheng Yin Yimin Qin Peili Shen Qiang Peng A Novel Pectic Polysaccharide of Jujube Pomace: Structural Analysis and Intracellular Antioxidant Activities Antioxidants polysaccharide jujube pomace structural analysis antioxidant activity |
| title | A Novel Pectic Polysaccharide of Jujube Pomace: Structural Analysis and Intracellular Antioxidant Activities |
| title_full | A Novel Pectic Polysaccharide of Jujube Pomace: Structural Analysis and Intracellular Antioxidant Activities |
| title_fullStr | A Novel Pectic Polysaccharide of Jujube Pomace: Structural Analysis and Intracellular Antioxidant Activities |
| title_full_unstemmed | A Novel Pectic Polysaccharide of Jujube Pomace: Structural Analysis and Intracellular Antioxidant Activities |
| title_short | A Novel Pectic Polysaccharide of Jujube Pomace: Structural Analysis and Intracellular Antioxidant Activities |
| title_sort | novel pectic polysaccharide of jujube pomace structural analysis and intracellular antioxidant activities |
| topic | polysaccharide jujube pomace structural analysis antioxidant activity |
| url | https://www.mdpi.com/2076-3921/9/2/127 |
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