Therapeutic effects of 3,3′-diindolylmethane on the alveolar bone in mice with experimentally induced periodontitis
In this study, we investigated effects of 3,3′-diindolylmethane (DIM) on alveolar bone resorption in mice with experimentally induced periodontitis. Experimental periodontitis was induced by placing 5.0 silk sutures around the maxillary left second molar for 1 week. Fifty-three SPF, 6–8-week-old, fe...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-05-01
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| Series: | Biotechnology & Biotechnological Equipment |
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| Online Access: | http://dx.doi.org/10.1080/13102818.2018.1438853 |
| _version_ | 1818516207853109248 |
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| author | Jingjing Pan Qianzhou Jiang Hongbing Guan Jinquan He Baishun Li |
| author_facet | Jingjing Pan Qianzhou Jiang Hongbing Guan Jinquan He Baishun Li |
| author_sort | Jingjing Pan |
| collection | DOAJ |
| description | In this study, we investigated effects of 3,3′-diindolylmethane (DIM) on alveolar bone resorption in mice with experimentally induced periodontitis. Experimental periodontitis was induced by placing 5.0 silk sutures around the maxillary left second molar for 1 week. Fifty-three SPF, 6–8-week-old, female C57 mice were randomly divided into four groups: (1) healthy mice (control group), (2) mice with experimentally induced periodontitis (Ped group), (3) mice with periodontitis treated with corn oil (OIL-Ped group) and (4) mice with periodontitis treated with DIM dissolved in corn oil (DIM-Ped group). Infiltration of inflammatory cells in the gingival tissue was assessed via histomorphometrical analysis, inflammatory factors were detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR) and changes in the alveolar bone were detected by micro-computed tomography (micro-CT) after treatment for 1 and 4 weeks. After 1 week of treatment, compared with the Ped and OIL-Ped groups, the DIM-Ped group showed decreased infiltration of inflammatory cells, preservation of connective tissue in the papilla and less interproximal and interradicular bone resorption. In the DIM-Ped group, the expression of IL-1β and RANKL decreased significantly (P < 0.05). After 4 weeks of treatment, periodontal inflammation level and alveolar bone absorption were similar among groups. However, in the DIM-Ped group, the expression of IL-1β, IL-6 and RANK significantly decreased (P < 0.05). As a whole, DIM treatment for 1 week can benefit alveolar bone recovery by inhibiting the inflammation of the periodontal tissue and expression of bone resorption-related cytokines. |
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| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-12-11T00:39:01Z |
| publishDate | 2018-05-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-dee7d35764aa4d6e9797f50efa3788a72022-12-22T01:27:00ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302018-05-0132369270010.1080/13102818.2018.14388531438853Therapeutic effects of 3,3′-diindolylmethane on the alveolar bone in mice with experimentally induced periodontitisJingjing Pan0Qianzhou Jiang1Hongbing Guan2Jinquan He3Baishun Li4Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical UniversityGuangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical UniversityGuangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical UniversityGuangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical UniversityGuangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical UniversityIn this study, we investigated effects of 3,3′-diindolylmethane (DIM) on alveolar bone resorption in mice with experimentally induced periodontitis. Experimental periodontitis was induced by placing 5.0 silk sutures around the maxillary left second molar for 1 week. Fifty-three SPF, 6–8-week-old, female C57 mice were randomly divided into four groups: (1) healthy mice (control group), (2) mice with experimentally induced periodontitis (Ped group), (3) mice with periodontitis treated with corn oil (OIL-Ped group) and (4) mice with periodontitis treated with DIM dissolved in corn oil (DIM-Ped group). Infiltration of inflammatory cells in the gingival tissue was assessed via histomorphometrical analysis, inflammatory factors were detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR) and changes in the alveolar bone were detected by micro-computed tomography (micro-CT) after treatment for 1 and 4 weeks. After 1 week of treatment, compared with the Ped and OIL-Ped groups, the DIM-Ped group showed decreased infiltration of inflammatory cells, preservation of connective tissue in the papilla and less interproximal and interradicular bone resorption. In the DIM-Ped group, the expression of IL-1β and RANKL decreased significantly (P < 0.05). After 4 weeks of treatment, periodontal inflammation level and alveolar bone absorption were similar among groups. However, in the DIM-Ped group, the expression of IL-1β, IL-6 and RANK significantly decreased (P < 0.05). As a whole, DIM treatment for 1 week can benefit alveolar bone recovery by inhibiting the inflammation of the periodontal tissue and expression of bone resorption-related cytokines.http://dx.doi.org/10.1080/13102818.2018.14388533,3′-diindolylmethaneperiodontitisbone resorptioncytokines |
| spellingShingle | Jingjing Pan Qianzhou Jiang Hongbing Guan Jinquan He Baishun Li Therapeutic effects of 3,3′-diindolylmethane on the alveolar bone in mice with experimentally induced periodontitis Biotechnology & Biotechnological Equipment 3,3′-diindolylmethane periodontitis bone resorption cytokines |
| title | Therapeutic effects of 3,3′-diindolylmethane on the alveolar bone in mice with experimentally induced periodontitis |
| title_full | Therapeutic effects of 3,3′-diindolylmethane on the alveolar bone in mice with experimentally induced periodontitis |
| title_fullStr | Therapeutic effects of 3,3′-diindolylmethane on the alveolar bone in mice with experimentally induced periodontitis |
| title_full_unstemmed | Therapeutic effects of 3,3′-diindolylmethane on the alveolar bone in mice with experimentally induced periodontitis |
| title_short | Therapeutic effects of 3,3′-diindolylmethane on the alveolar bone in mice with experimentally induced periodontitis |
| title_sort | therapeutic effects of 3 3 diindolylmethane on the alveolar bone in mice with experimentally induced periodontitis |
| topic | 3,3′-diindolylmethane periodontitis bone resorption cytokines |
| url | http://dx.doi.org/10.1080/13102818.2018.1438853 |
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