Increased expression of collagens, transforming growth factor-β1, and -β3 in gluteal muscle contracture
<p>Abstract</p> <p>Backgroud</p> <p>Gluteal muscle contracture (GMC) is a multi-factor human chronic fibrotic disease of the gluteal muscle. Fibrotic tissue is characterized by excessive accumulation of collagen in the muscle's extracellular matrix. Transforming gr...
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| Format: | Article |
| Language: | English |
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BMC
2010-01-01
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| Series: | BMC Musculoskeletal Disorders |
| Online Access: | http://www.biomedcentral.com/1471-2474/11/15 |
| _version_ | 1818919334110560256 |
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| author | Li Hao-Peng Lu Bin He Xi-Jing Zhao Chen-Guang Kang An-Jing |
| author_facet | Li Hao-Peng Lu Bin He Xi-Jing Zhao Chen-Guang Kang An-Jing |
| author_sort | Li Hao-Peng |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Backgroud</p> <p>Gluteal muscle contracture (GMC) is a multi-factor human chronic fibrotic disease of the gluteal muscle. Fibrotic tissue is characterized by excessive accumulation of collagen in the muscle's extracellular matrix. Transforming growth factor (TGF)-β1 and -β2 are thought to play an important role in fibrogenesis, while TGF-β3 is believed to have an anti-fibrotic function. We hypothesize that the expression of collagen and TGF-βs would be up-regulated in GMC patients.</p> <p>Methods</p> <p>The expression of collagen type I, type III and TGF-βs were studied in 23 fibrotic samples and 23 normal/control samples in GMC patients using immunohistochemistry, reverse transcription and polymerase chain reaction (RT-PCR) and western bolt analysis.</p> <p>Results</p> <p>Compared to the unaffected adjacent muscle, increased expression of TGF-β1 and -β3 was associated with deposition of collagen type I and type III in the fibrotic muscle of the GMC patients at the mRNA level. Strong up-regulation of these proteins in fibrotic muscle was confirmed by immunohistochemical staining and western blot analysis. TGF-β2 was not up-regulated in relation to GMC.</p> <p>Conclusion</p> <p>This study confirmed our hypothesis that collagen types I, III, TGF-β1 and TGF-β3 were up-regulated in biopsy specimens obtained from patients with GMC. Complex interaction of TGF-β1 with profibrotic function and TGF-β3 with antifibrotic function may increase synthesis of collagens and thereby significantly contribute to the process of gluteal muscle scarring in patients with GMC.</p> |
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| format | Article |
| id | doaj.art-dee832d45cde41e5bf79c6e528aa9662 |
| institution | Directory Open Access Journal |
| issn | 1471-2474 |
| language | English |
| last_indexed | 2024-12-20T01:04:12Z |
| publishDate | 2010-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Musculoskeletal Disorders |
| spelling | doaj.art-dee832d45cde41e5bf79c6e528aa96622022-12-21T19:58:55ZengBMCBMC Musculoskeletal Disorders1471-24742010-01-011111510.1186/1471-2474-11-15Increased expression of collagens, transforming growth factor-β1, and -β3 in gluteal muscle contractureLi Hao-PengLu BinHe Xi-JingZhao Chen-GuangKang An-Jing<p>Abstract</p> <p>Backgroud</p> <p>Gluteal muscle contracture (GMC) is a multi-factor human chronic fibrotic disease of the gluteal muscle. Fibrotic tissue is characterized by excessive accumulation of collagen in the muscle's extracellular matrix. Transforming growth factor (TGF)-β1 and -β2 are thought to play an important role in fibrogenesis, while TGF-β3 is believed to have an anti-fibrotic function. We hypothesize that the expression of collagen and TGF-βs would be up-regulated in GMC patients.</p> <p>Methods</p> <p>The expression of collagen type I, type III and TGF-βs were studied in 23 fibrotic samples and 23 normal/control samples in GMC patients using immunohistochemistry, reverse transcription and polymerase chain reaction (RT-PCR) and western bolt analysis.</p> <p>Results</p> <p>Compared to the unaffected adjacent muscle, increased expression of TGF-β1 and -β3 was associated with deposition of collagen type I and type III in the fibrotic muscle of the GMC patients at the mRNA level. Strong up-regulation of these proteins in fibrotic muscle was confirmed by immunohistochemical staining and western blot analysis. TGF-β2 was not up-regulated in relation to GMC.</p> <p>Conclusion</p> <p>This study confirmed our hypothesis that collagen types I, III, TGF-β1 and TGF-β3 were up-regulated in biopsy specimens obtained from patients with GMC. Complex interaction of TGF-β1 with profibrotic function and TGF-β3 with antifibrotic function may increase synthesis of collagens and thereby significantly contribute to the process of gluteal muscle scarring in patients with GMC.</p>http://www.biomedcentral.com/1471-2474/11/15 |
| spellingShingle | Li Hao-Peng Lu Bin He Xi-Jing Zhao Chen-Guang Kang An-Jing Increased expression of collagens, transforming growth factor-β1, and -β3 in gluteal muscle contracture BMC Musculoskeletal Disorders |
| title | Increased expression of collagens, transforming growth factor-β1, and -β3 in gluteal muscle contracture |
| title_full | Increased expression of collagens, transforming growth factor-β1, and -β3 in gluteal muscle contracture |
| title_fullStr | Increased expression of collagens, transforming growth factor-β1, and -β3 in gluteal muscle contracture |
| title_full_unstemmed | Increased expression of collagens, transforming growth factor-β1, and -β3 in gluteal muscle contracture |
| title_short | Increased expression of collagens, transforming growth factor-β1, and -β3 in gluteal muscle contracture |
| title_sort | increased expression of collagens transforming growth factor β1 and β3 in gluteal muscle contracture |
| url | http://www.biomedcentral.com/1471-2474/11/15 |
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