ISOLATION AND CLONING OF cDNA OF GENE ENCODING FOR METALLOTHIONEIN TYPE 2 FROM MELASTOMA AFFINE

Metallothionein is an important protein for detoxifying heavy metal ions. h is research was conducted to isolate and clone cDNA of gene encoding for metallothionein type 2 from Melastoma affi ne . Total RNA was isolated from young leaves. Total cDNA was synthesized from the total RNA by reverse tr...

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Main Authors: UTUT WIDYASTUT, LULUT DWI SULISTYANINGSIH, NIKEN TRISNANINGRUM, SUHARSONO
Format: Article
Language:English
Published: SEAMEO, BIOTROP 2009-01-01
Series:Biotropia: The Southeast Asian Journal of Tropical Biology
Subjects:
Online Access:http://journal.biotrop.org/index.php/biotropia/article/view/64/43
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author UTUT WIDYASTUT
LULUT DWI SULISTYANINGSIH
NIKEN TRISNANINGRUM
SUHARSONO
author_facet UTUT WIDYASTUT
LULUT DWI SULISTYANINGSIH
NIKEN TRISNANINGRUM
SUHARSONO
author_sort UTUT WIDYASTUT
collection DOAJ
description Metallothionein is an important protein for detoxifying heavy metal ions. h is research was conducted to isolate and clone cDNA of gene encoding for metallothionein type 2 from Melastoma affi ne . Total RNA was isolated from young leaves. Total cDNA was synthesized from the total RNA by reverse transcription. h e MaMt2 cDNA was successfully isolated by PCR technique. h e MaMt2 cDNA was inserted into pGEM-T Easy and the recombinant plasmid was successfully introduced into Escherichia coli DH5 α . DNA sequencing analysis showed that this cDNA is full length consisting of 246 pb encoding 81 amino acid residues. h is cDNA is identical to mRNA of AtMt2 from Arabidopsis thaliana. It does not contain any restriction sites found in the cloning sites of pGEM-T Easy. h e deduced protein of MaMT2 contains 14 cysteine residues distributed in the Cys-Cys, Cys-X-Cys, and Cys-X-X-Cys motifs
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spelling doaj.art-dee883e4da0040a090b484ce55aaee412022-12-21T23:56:09ZengSEAMEO, BIOTROPBiotropia: The Southeast Asian Journal of Tropical Biology0215-63341907-770X2009-01-011612837ISOLATION AND CLONING OF cDNA OF GENE ENCODING FOR METALLOTHIONEIN TYPE 2 FROM MELASTOMA AFFINEUTUT WIDYASTUTLULUT DWI SULISTYANINGSIHNIKEN TRISNANINGRUMSUHARSONOMetallothionein is an important protein for detoxifying heavy metal ions. h is research was conducted to isolate and clone cDNA of gene encoding for metallothionein type 2 from Melastoma affi ne . Total RNA was isolated from young leaves. Total cDNA was synthesized from the total RNA by reverse transcription. h e MaMt2 cDNA was successfully isolated by PCR technique. h e MaMt2 cDNA was inserted into pGEM-T Easy and the recombinant plasmid was successfully introduced into Escherichia coli DH5 α . DNA sequencing analysis showed that this cDNA is full length consisting of 246 pb encoding 81 amino acid residues. h is cDNA is identical to mRNA of AtMt2 from Arabidopsis thaliana. It does not contain any restriction sites found in the cloning sites of pGEM-T Easy. h e deduced protein of MaMT2 contains 14 cysteine residues distributed in the Cys-Cys, Cys-X-Cys, and Cys-X-X-Cys motifshttp://journal.biotrop.org/index.php/biotropia/article/view/64/43cDNAmetallothioneinMelastoma affinecloningcysteine
spellingShingle UTUT WIDYASTUT
LULUT DWI SULISTYANINGSIH
NIKEN TRISNANINGRUM
SUHARSONO
ISOLATION AND CLONING OF cDNA OF GENE ENCODING FOR METALLOTHIONEIN TYPE 2 FROM MELASTOMA AFFINE
Biotropia: The Southeast Asian Journal of Tropical Biology
cDNA
metallothionein
Melastoma affine
cloning
cysteine
title ISOLATION AND CLONING OF cDNA OF GENE ENCODING FOR METALLOTHIONEIN TYPE 2 FROM MELASTOMA AFFINE
title_full ISOLATION AND CLONING OF cDNA OF GENE ENCODING FOR METALLOTHIONEIN TYPE 2 FROM MELASTOMA AFFINE
title_fullStr ISOLATION AND CLONING OF cDNA OF GENE ENCODING FOR METALLOTHIONEIN TYPE 2 FROM MELASTOMA AFFINE
title_full_unstemmed ISOLATION AND CLONING OF cDNA OF GENE ENCODING FOR METALLOTHIONEIN TYPE 2 FROM MELASTOMA AFFINE
title_short ISOLATION AND CLONING OF cDNA OF GENE ENCODING FOR METALLOTHIONEIN TYPE 2 FROM MELASTOMA AFFINE
title_sort isolation and cloning of cdna of gene encoding for metallothionein type 2 from melastoma affine
topic cDNA
metallothionein
Melastoma affine
cloning
cysteine
url http://journal.biotrop.org/index.php/biotropia/article/view/64/43
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AT nikentrisnaningrum isolationandcloningofcdnaofgeneencodingformetallothioneintype2frommelastomaaffine
AT suharsono isolationandcloningofcdnaofgeneencodingformetallothioneintype2frommelastomaaffine