ISOLATION AND CLONING OF cDNA OF GENE ENCODING FOR METALLOTHIONEIN TYPE 2 FROM MELASTOMA AFFINE
Metallothionein is an important protein for detoxifying heavy metal ions. h is research was conducted to isolate and clone cDNA of gene encoding for metallothionein type 2 from Melastoma affi ne . Total RNA was isolated from young leaves. Total cDNA was synthesized from the total RNA by reverse tr...
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Format: | Article |
Language: | English |
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SEAMEO, BIOTROP
2009-01-01
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Series: | Biotropia: The Southeast Asian Journal of Tropical Biology |
Subjects: | |
Online Access: | http://journal.biotrop.org/index.php/biotropia/article/view/64/43 |
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author | UTUT WIDYASTUT LULUT DWI SULISTYANINGSIH NIKEN TRISNANINGRUM SUHARSONO |
author_facet | UTUT WIDYASTUT LULUT DWI SULISTYANINGSIH NIKEN TRISNANINGRUM SUHARSONO |
author_sort | UTUT WIDYASTUT |
collection | DOAJ |
description | Metallothionein is an important protein for detoxifying heavy metal ions. h is research was conducted to isolate and clone cDNA of gene encoding for metallothionein type 2 from Melastoma affi ne . Total RNA was isolated from young leaves. Total cDNA was synthesized from the total RNA by reverse transcription. h e MaMt2 cDNA was successfully isolated by PCR technique. h e MaMt2 cDNA was inserted into pGEM-T Easy and the recombinant plasmid was successfully introduced into Escherichia coli DH5 α . DNA sequencing analysis showed that this cDNA is full length consisting of 246 pb encoding 81 amino acid residues. h is cDNA is identical to mRNA of AtMt2 from Arabidopsis thaliana. It does not contain any restriction sites found in the cloning sites of pGEM-T Easy. h e deduced protein of MaMT2 contains 14 cysteine residues distributed in the Cys-Cys, Cys-X-Cys, and Cys-X-X-Cys motifs |
first_indexed | 2024-12-13T06:50:37Z |
format | Article |
id | doaj.art-dee883e4da0040a090b484ce55aaee41 |
institution | Directory Open Access Journal |
issn | 0215-6334 1907-770X |
language | English |
last_indexed | 2024-12-13T06:50:37Z |
publishDate | 2009-01-01 |
publisher | SEAMEO, BIOTROP |
record_format | Article |
series | Biotropia: The Southeast Asian Journal of Tropical Biology |
spelling | doaj.art-dee883e4da0040a090b484ce55aaee412022-12-21T23:56:09ZengSEAMEO, BIOTROPBiotropia: The Southeast Asian Journal of Tropical Biology0215-63341907-770X2009-01-011612837ISOLATION AND CLONING OF cDNA OF GENE ENCODING FOR METALLOTHIONEIN TYPE 2 FROM MELASTOMA AFFINEUTUT WIDYASTUTLULUT DWI SULISTYANINGSIHNIKEN TRISNANINGRUMSUHARSONOMetallothionein is an important protein for detoxifying heavy metal ions. h is research was conducted to isolate and clone cDNA of gene encoding for metallothionein type 2 from Melastoma affi ne . Total RNA was isolated from young leaves. Total cDNA was synthesized from the total RNA by reverse transcription. h e MaMt2 cDNA was successfully isolated by PCR technique. h e MaMt2 cDNA was inserted into pGEM-T Easy and the recombinant plasmid was successfully introduced into Escherichia coli DH5 α . DNA sequencing analysis showed that this cDNA is full length consisting of 246 pb encoding 81 amino acid residues. h is cDNA is identical to mRNA of AtMt2 from Arabidopsis thaliana. It does not contain any restriction sites found in the cloning sites of pGEM-T Easy. h e deduced protein of MaMT2 contains 14 cysteine residues distributed in the Cys-Cys, Cys-X-Cys, and Cys-X-X-Cys motifshttp://journal.biotrop.org/index.php/biotropia/article/view/64/43cDNAmetallothioneinMelastoma affinecloningcysteine |
spellingShingle | UTUT WIDYASTUT LULUT DWI SULISTYANINGSIH NIKEN TRISNANINGRUM SUHARSONO ISOLATION AND CLONING OF cDNA OF GENE ENCODING FOR METALLOTHIONEIN TYPE 2 FROM MELASTOMA AFFINE Biotropia: The Southeast Asian Journal of Tropical Biology cDNA metallothionein Melastoma affine cloning cysteine |
title | ISOLATION AND CLONING OF cDNA OF GENE ENCODING FOR METALLOTHIONEIN TYPE 2 FROM MELASTOMA AFFINE |
title_full | ISOLATION AND CLONING OF cDNA OF GENE ENCODING FOR METALLOTHIONEIN TYPE 2 FROM MELASTOMA AFFINE |
title_fullStr | ISOLATION AND CLONING OF cDNA OF GENE ENCODING FOR METALLOTHIONEIN TYPE 2 FROM MELASTOMA AFFINE |
title_full_unstemmed | ISOLATION AND CLONING OF cDNA OF GENE ENCODING FOR METALLOTHIONEIN TYPE 2 FROM MELASTOMA AFFINE |
title_short | ISOLATION AND CLONING OF cDNA OF GENE ENCODING FOR METALLOTHIONEIN TYPE 2 FROM MELASTOMA AFFINE |
title_sort | isolation and cloning of cdna of gene encoding for metallothionein type 2 from melastoma affine |
topic | cDNA metallothionein Melastoma affine cloning cysteine |
url | http://journal.biotrop.org/index.php/biotropia/article/view/64/43 |
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