| Summary: | The Sox family plays essential roles as transcription factors in vertebrates; however, little is known about the Sox family in <i>Lutraria sieboldii</i>. <i>L. sieboldii</i> are pleasant to eat with a short growth cycle and have become one of the best bottom-seeded enrichment species in Guang Xi. In this study, Sox2 (named LsSox2) and Sox9 (named LsSox9) from <i>L. sieboldii</i> were cloned, and their expression patterns were analyzed. The length of the <i>LsSox</i>2 gene coding sequence was 1011 bp, encoding 336 amino acids, and <i>LsSox</i>9 was 1449 bp, encoding 482 amino acids. <i>LsSox</i>2 had its highest expression levels in the ovary, which were 356 times those in testis, whereas <i>LsSox</i>9 presented higher expression in testis, which was 6 times more highly expressed than in the ovary. <i>LsSox</i>2 exhibited the highest expression during the morula stage, which was 20 times that of the D-shaped larvae or zygote. <i>LsSox</i>9 exhibited two expression peaks, one at the four-cell stage and the other at the trochophore stage, while the lowest expression was in the zygote. <i>LsSox</i>9 was 73 times more highly expressed in the four-cell stage than in the zygote stage. During gonadal development, <i>LsSox</i>2 presented the highest expression in the mature ovary, which was 756 times more highly expressed than in mature testis. <i>LsSox</i>9 presented higher expression in testis at the emission stage which was 6 times more highly expressed than in the ovary. These results indicate that <i>LsSox</i>2 and <i>LsSox</i>9 may play important roles in embryonic and gonadal development.
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