Cloning and Expression of Sox2 and Sox9 in Embryonic and Gonadal Development of <i>Lutraria sieboldii</i>
The Sox family plays essential roles as transcription factors in vertebrates; however, little is known about the Sox family in <i>Lutraria sieboldii</i>. <i>L. sieboldii</i> are pleasant to eat with a short growth cycle and have become one of the best bottom-seeded enrichment...
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MDPI AG
2022-12-01
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| Series: | Fishes |
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| Online Access: | https://www.mdpi.com/2410-3888/7/6/392 |
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| author | Min Lu Zenghou Xing Yurui Zhou Youhou Xu Huijing Peng Jie Zou Solomon Felix Dan Zhicai She Pengliang Wang Jinfeng Liu Shaomin Qin Jialin Yang Peng Zhu |
| author_facet | Min Lu Zenghou Xing Yurui Zhou Youhou Xu Huijing Peng Jie Zou Solomon Felix Dan Zhicai She Pengliang Wang Jinfeng Liu Shaomin Qin Jialin Yang Peng Zhu |
| author_sort | Min Lu |
| collection | DOAJ |
| description | The Sox family plays essential roles as transcription factors in vertebrates; however, little is known about the Sox family in <i>Lutraria sieboldii</i>. <i>L. sieboldii</i> are pleasant to eat with a short growth cycle and have become one of the best bottom-seeded enrichment species in Guang Xi. In this study, Sox2 (named LsSox2) and Sox9 (named LsSox9) from <i>L. sieboldii</i> were cloned, and their expression patterns were analyzed. The length of the <i>LsSox</i>2 gene coding sequence was 1011 bp, encoding 336 amino acids, and <i>LsSox</i>9 was 1449 bp, encoding 482 amino acids. <i>LsSox</i>2 had its highest expression levels in the ovary, which were 356 times those in testis, whereas <i>LsSox</i>9 presented higher expression in testis, which was 6 times more highly expressed than in the ovary. <i>LsSox</i>2 exhibited the highest expression during the morula stage, which was 20 times that of the D-shaped larvae or zygote. <i>LsSox</i>9 exhibited two expression peaks, one at the four-cell stage and the other at the trochophore stage, while the lowest expression was in the zygote. <i>LsSox</i>9 was 73 times more highly expressed in the four-cell stage than in the zygote stage. During gonadal development, <i>LsSox</i>2 presented the highest expression in the mature ovary, which was 756 times more highly expressed than in mature testis. <i>LsSox</i>9 presented higher expression in testis at the emission stage which was 6 times more highly expressed than in the ovary. These results indicate that <i>LsSox</i>2 and <i>LsSox</i>9 may play important roles in embryonic and gonadal development. |
| first_indexed | 2024-03-09T16:44:25Z |
| format | Article |
| id | doaj.art-dee8efbb48b24fc6a6b5ef01e8dd799e |
| institution | Directory Open Access Journal |
| issn | 2410-3888 |
| language | English |
| last_indexed | 2024-03-09T16:44:25Z |
| publishDate | 2022-12-01 |
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| series | Fishes |
| spelling | doaj.art-dee8efbb48b24fc6a6b5ef01e8dd799e2023-11-24T14:49:00ZengMDPI AGFishes2410-38882022-12-017639210.3390/fishes7060392Cloning and Expression of Sox2 and Sox9 in Embryonic and Gonadal Development of <i>Lutraria sieboldii</i>Min Lu0Zenghou Xing1Yurui Zhou2Youhou Xu3Huijing Peng4Jie Zou5Solomon Felix Dan6Zhicai She7Pengliang Wang8Jinfeng Liu9Shaomin Qin10Jialin Yang11Peng Zhu12Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Beibu Gulf University, Qinzhou 535011, ChinaGuangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Beibu Gulf University, Qinzhou 535011, ChinaGuangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Beibu Gulf University, Qinzhou 535011, ChinaGuangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Beibu Gulf University, Qinzhou 535011, ChinaGuangxi Institute of Oceanology, Beihai 536000, ChinaGuangxi Institute of Oceanology, Beihai 536000, ChinaGuangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Beibu Gulf University, Qinzhou 535011, ChinaGuangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Beibu Gulf University, Qinzhou 535011, ChinaGuangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Beibu Gulf University, Qinzhou 535011, ChinaGuangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning 530001, ChinaGuangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning 530001, ChinaGuangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Beibu Gulf University, Qinzhou 535011, ChinaGuangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Beibu Gulf University, Qinzhou 535011, ChinaThe Sox family plays essential roles as transcription factors in vertebrates; however, little is known about the Sox family in <i>Lutraria sieboldii</i>. <i>L. sieboldii</i> are pleasant to eat with a short growth cycle and have become one of the best bottom-seeded enrichment species in Guang Xi. In this study, Sox2 (named LsSox2) and Sox9 (named LsSox9) from <i>L. sieboldii</i> were cloned, and their expression patterns were analyzed. The length of the <i>LsSox</i>2 gene coding sequence was 1011 bp, encoding 336 amino acids, and <i>LsSox</i>9 was 1449 bp, encoding 482 amino acids. <i>LsSox</i>2 had its highest expression levels in the ovary, which were 356 times those in testis, whereas <i>LsSox</i>9 presented higher expression in testis, which was 6 times more highly expressed than in the ovary. <i>LsSox</i>2 exhibited the highest expression during the morula stage, which was 20 times that of the D-shaped larvae or zygote. <i>LsSox</i>9 exhibited two expression peaks, one at the four-cell stage and the other at the trochophore stage, while the lowest expression was in the zygote. <i>LsSox</i>9 was 73 times more highly expressed in the four-cell stage than in the zygote stage. During gonadal development, <i>LsSox</i>2 presented the highest expression in the mature ovary, which was 756 times more highly expressed than in mature testis. <i>LsSox</i>9 presented higher expression in testis at the emission stage which was 6 times more highly expressed than in the ovary. These results indicate that <i>LsSox</i>2 and <i>LsSox</i>9 may play important roles in embryonic and gonadal development.https://www.mdpi.com/2410-3888/7/6/392<i>Lutraria sieboldii</i>LsSox2LsSox9expressioncloning |
| spellingShingle | Min Lu Zenghou Xing Yurui Zhou Youhou Xu Huijing Peng Jie Zou Solomon Felix Dan Zhicai She Pengliang Wang Jinfeng Liu Shaomin Qin Jialin Yang Peng Zhu Cloning and Expression of Sox2 and Sox9 in Embryonic and Gonadal Development of <i>Lutraria sieboldii</i> Fishes <i>Lutraria sieboldii</i> LsSox2 LsSox9 expression cloning |
| title | Cloning and Expression of Sox2 and Sox9 in Embryonic and Gonadal Development of <i>Lutraria sieboldii</i> |
| title_full | Cloning and Expression of Sox2 and Sox9 in Embryonic and Gonadal Development of <i>Lutraria sieboldii</i> |
| title_fullStr | Cloning and Expression of Sox2 and Sox9 in Embryonic and Gonadal Development of <i>Lutraria sieboldii</i> |
| title_full_unstemmed | Cloning and Expression of Sox2 and Sox9 in Embryonic and Gonadal Development of <i>Lutraria sieboldii</i> |
| title_short | Cloning and Expression of Sox2 and Sox9 in Embryonic and Gonadal Development of <i>Lutraria sieboldii</i> |
| title_sort | cloning and expression of sox2 and sox9 in embryonic and gonadal development of i lutraria sieboldii i |
| topic | <i>Lutraria sieboldii</i> LsSox2 LsSox9 expression cloning |
| url | https://www.mdpi.com/2410-3888/7/6/392 |
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