The Responsiveness of Bee Venom Phospholipase A2 on Regulatory T Cells Correlates with the CD11c<sup>+</sup>CD206<sup>+</sup>Population in Human Peripheral Blood Mononuclear Cells
Bee venom phospholipase A2 (bvPLA2) has been reported to have therapeutic effects such as neuroprotection, anti-inflammation, anti-nociception, anti-cancer properties, caused by increasing regulatory T cells (Tregs). The mechanism of Tregs modulation by bvPLA2 has been demonstrated by binding with t...
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MDPI AG
2021-10-01
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| author | Heejin Jo Hyunjung Baek Seon-Young Park Bonhyuk Goo Woo-Sang Jung Hyunsu Bae Sang-Soo Nam |
| author_facet | Heejin Jo Hyunjung Baek Seon-Young Park Bonhyuk Goo Woo-Sang Jung Hyunsu Bae Sang-Soo Nam |
| author_sort | Heejin Jo |
| collection | DOAJ |
| description | Bee venom phospholipase A2 (bvPLA2) has been reported to have therapeutic effects such as neuroprotection, anti-inflammation, anti-nociception, anti-cancer properties, caused by increasing regulatory T cells (Tregs). The mechanism of Tregs modulation by bvPLA2 has been demonstrated by binding with the mannose receptor, CD206 in experimental models of several diseases. However, it remains unknown whether this mechanism can also be applied in human blood. In this study, we collected peripheral blood samples from healthy donors and analyzed the percentages of monocyte-derived dendritic cells with CD206 (CD206<sup>+</sup> DCs) before expansion, the proportion of Tregs, and the subpopulations after expansion treated with bvPLA2 or PBS using flow cytometry and the correlations among them. The percentage of Tregs tended to be higher in the bvPLA2 group than in the control group. There were significant positive correlations between the CD206 population in hPBMC and the proportions of Tregs treated with bvPLA2, especially in the Treg fold change comparing the increase ratio of Tregs in bvPLA2 and in PBS. These findings indicate that bvPLA2 increased the proportion of Tregs in healthy human peripheral blood and the number of CD206<sup>+</sup> DCs could be a predictor of the bvPLA2 response of different individuals. |
| first_indexed | 2024-03-10T06:09:55Z |
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| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-10T06:09:55Z |
| publishDate | 2021-10-01 |
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| spelling | doaj.art-deeb7235289049b1a08c686815d543bb2023-11-22T20:13:22ZengMDPI AGToxins2072-66512021-10-01131071710.3390/toxins13100717The Responsiveness of Bee Venom Phospholipase A2 on Regulatory T Cells Correlates with the CD11c<sup>+</sup>CD206<sup>+</sup>Population in Human Peripheral Blood Mononuclear CellsHeejin Jo0Hyunjung Baek1Seon-Young Park2Bonhyuk Goo3Woo-Sang Jung4Hyunsu Bae5Sang-Soo Nam6Chaum Life Center, Department of Korean Medicine, CHA University School of Medicine, Seoul 06062, KoreaDepartment of Physiology, College of Korean Medicine, Kyung Hee University, Seoul 02453, KoreaDepartment of Physiology, College of Korean Medicine, Kyung Hee University, Seoul 02453, KoreaDepartment of Acupuncture & Moxibustion, Kyung Hee University Hospital at Gangdong, Seoul 05278, KoreaDepartment of Cardiology and Neurology, College of Korean Medicine, Kyung Hee University, Seoul 02447, KoreaDepartment of Physiology, College of Korean Medicine, Kyung Hee University, Seoul 02453, KoreaDepartment of Acupuncture & Moxibustion, Kyung Hee University Hospital at Gangdong, Seoul 05278, KoreaBee venom phospholipase A2 (bvPLA2) has been reported to have therapeutic effects such as neuroprotection, anti-inflammation, anti-nociception, anti-cancer properties, caused by increasing regulatory T cells (Tregs). The mechanism of Tregs modulation by bvPLA2 has been demonstrated by binding with the mannose receptor, CD206 in experimental models of several diseases. However, it remains unknown whether this mechanism can also be applied in human blood. In this study, we collected peripheral blood samples from healthy donors and analyzed the percentages of monocyte-derived dendritic cells with CD206 (CD206<sup>+</sup> DCs) before expansion, the proportion of Tregs, and the subpopulations after expansion treated with bvPLA2 or PBS using flow cytometry and the correlations among them. The percentage of Tregs tended to be higher in the bvPLA2 group than in the control group. There were significant positive correlations between the CD206 population in hPBMC and the proportions of Tregs treated with bvPLA2, especially in the Treg fold change comparing the increase ratio of Tregs in bvPLA2 and in PBS. These findings indicate that bvPLA2 increased the proportion of Tregs in healthy human peripheral blood and the number of CD206<sup>+</sup> DCs could be a predictor of the bvPLA2 response of different individuals.https://www.mdpi.com/2072-6651/13/10/717regulatory T cell (Treg)bee venom phospholipase A2 (bvPLA2)human peripheral blood mononuclear cell (hPBMC)CD206mannose receptor |
| spellingShingle | Heejin Jo Hyunjung Baek Seon-Young Park Bonhyuk Goo Woo-Sang Jung Hyunsu Bae Sang-Soo Nam The Responsiveness of Bee Venom Phospholipase A2 on Regulatory T Cells Correlates with the CD11c<sup>+</sup>CD206<sup>+</sup>Population in Human Peripheral Blood Mononuclear Cells Toxins regulatory T cell (Treg) bee venom phospholipase A2 (bvPLA2) human peripheral blood mononuclear cell (hPBMC) CD206 mannose receptor |
| title | The Responsiveness of Bee Venom Phospholipase A2 on Regulatory T Cells Correlates with the CD11c<sup>+</sup>CD206<sup>+</sup>Population in Human Peripheral Blood Mononuclear Cells |
| title_full | The Responsiveness of Bee Venom Phospholipase A2 on Regulatory T Cells Correlates with the CD11c<sup>+</sup>CD206<sup>+</sup>Population in Human Peripheral Blood Mononuclear Cells |
| title_fullStr | The Responsiveness of Bee Venom Phospholipase A2 on Regulatory T Cells Correlates with the CD11c<sup>+</sup>CD206<sup>+</sup>Population in Human Peripheral Blood Mononuclear Cells |
| title_full_unstemmed | The Responsiveness of Bee Venom Phospholipase A2 on Regulatory T Cells Correlates with the CD11c<sup>+</sup>CD206<sup>+</sup>Population in Human Peripheral Blood Mononuclear Cells |
| title_short | The Responsiveness of Bee Venom Phospholipase A2 on Regulatory T Cells Correlates with the CD11c<sup>+</sup>CD206<sup>+</sup>Population in Human Peripheral Blood Mononuclear Cells |
| title_sort | responsiveness of bee venom phospholipase a2 on regulatory t cells correlates with the cd11c sup sup cd206 sup sup population in human peripheral blood mononuclear cells |
| topic | regulatory T cell (Treg) bee venom phospholipase A2 (bvPLA2) human peripheral blood mononuclear cell (hPBMC) CD206 mannose receptor |
| url | https://www.mdpi.com/2072-6651/13/10/717 |
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