Lipoxygenase inhibition and molecular docking studies of secondary metabolites from the leaves of Alstonia scholaris
The phytochemical study was carried out on the ethanolic extract of the leaves of Alstonia scholaris. This plant has been used traditionally for medicinal purposes in the treatment of several diseases such as cancer, jaundice, malaria, gastrointestinal troubles, dysentery, snake-bite and diarrhea. C...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | European Journal of Medicinal Chemistry Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772417423000080 |
| _version_ | 1797405338092175360 |
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| author | Salome Y. Ghansenyuy Kenneth O. Eyong Pierre Yemback Lateef Mehreen Vincent de Paul N. Nziko Muhammad S. Ali Gabriel N. Folefoc |
| author_facet | Salome Y. Ghansenyuy Kenneth O. Eyong Pierre Yemback Lateef Mehreen Vincent de Paul N. Nziko Muhammad S. Ali Gabriel N. Folefoc |
| author_sort | Salome Y. Ghansenyuy |
| collection | DOAJ |
| description | The phytochemical study was carried out on the ethanolic extract of the leaves of Alstonia scholaris. This plant has been used traditionally for medicinal purposes in the treatment of several diseases such as cancer, jaundice, malaria, gastrointestinal troubles, dysentery, snake-bite and diarrhea. Chromatographic purification of this extract led to the isolation and characterization of nine compounds. Their structures were elucidated as betulin (1), α-amyrin acetate (2), mixture of β-sitosterol (3) and stigmasterol (4), tetratriacontyl-trans-p-coumarate (5), ursolic acid (6), picralstonine (7), β-sitosterol glucoside (8) and scholaricine (9). Scholaricine and β-sitosterol-glucoside exhibited potent lipoxygenase inhibitory activity with IC50 of 15.2 ± 0.41 and 18.5 ± 0.14 respectively compared to Baicalein (22.6 ± 0.08) used as positive control. Molecular docking studies were conducted to support lipoxygenase inhibitory activities of the test compounds. |
| first_indexed | 2024-03-09T03:08:28Z |
| format | Article |
| id | doaj.art-deef0bee486a45c29955ef40823b6630 |
| institution | Directory Open Access Journal |
| issn | 2772-4174 |
| language | English |
| last_indexed | 2024-03-09T03:08:28Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | European Journal of Medicinal Chemistry Reports |
| spelling | doaj.art-deef0bee486a45c29955ef40823b66302023-12-04T05:25:07ZengElsevierEuropean Journal of Medicinal Chemistry Reports2772-41742023-12-019100108Lipoxygenase inhibition and molecular docking studies of secondary metabolites from the leaves of Alstonia scholarisSalome Y. Ghansenyuy0Kenneth O. Eyong1Pierre Yemback2Lateef Mehreen3Vincent de Paul N. Nziko4Muhammad S. Ali5Gabriel N. Folefoc6Department of Organic Chemistry, Faculty of Science, University of Yaounde I, P.O. Box 812, Yaounde, CameroonDepartment of Organic Chemistry, Faculty of Science, University of Yaounde I, P.O. Box 812, Yaounde, Cameroon; Corresponding author.Department of Organic Chemistry, Faculty of Science, University of Yaounde I, P.O. Box 812, Yaounde, CameroonDepartment of Biochemistry, Bahria University Medical and Dental College, Bahria University, Karachi, PakistanDepartment of Chemistry, Hampton University, Hampton, VA, 23668, USAH.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, 75270, PakistanDepartment of Organic Chemistry, Faculty of Science, University of Yaounde I, P.O. Box 812, Yaounde, CameroonThe phytochemical study was carried out on the ethanolic extract of the leaves of Alstonia scholaris. This plant has been used traditionally for medicinal purposes in the treatment of several diseases such as cancer, jaundice, malaria, gastrointestinal troubles, dysentery, snake-bite and diarrhea. Chromatographic purification of this extract led to the isolation and characterization of nine compounds. Their structures were elucidated as betulin (1), α-amyrin acetate (2), mixture of β-sitosterol (3) and stigmasterol (4), tetratriacontyl-trans-p-coumarate (5), ursolic acid (6), picralstonine (7), β-sitosterol glucoside (8) and scholaricine (9). Scholaricine and β-sitosterol-glucoside exhibited potent lipoxygenase inhibitory activity with IC50 of 15.2 ± 0.41 and 18.5 ± 0.14 respectively compared to Baicalein (22.6 ± 0.08) used as positive control. Molecular docking studies were conducted to support lipoxygenase inhibitory activities of the test compounds.http://www.sciencedirect.com/science/article/pii/S2772417423000080Alstonia scholarisLipoxygenaseCancerDocking studyβ-Sitosterol glucosideScholaricine |
| spellingShingle | Salome Y. Ghansenyuy Kenneth O. Eyong Pierre Yemback Lateef Mehreen Vincent de Paul N. Nziko Muhammad S. Ali Gabriel N. Folefoc Lipoxygenase inhibition and molecular docking studies of secondary metabolites from the leaves of Alstonia scholaris European Journal of Medicinal Chemistry Reports Alstonia scholaris Lipoxygenase Cancer Docking study β-Sitosterol glucoside Scholaricine |
| title | Lipoxygenase inhibition and molecular docking studies of secondary metabolites from the leaves of Alstonia scholaris |
| title_full | Lipoxygenase inhibition and molecular docking studies of secondary metabolites from the leaves of Alstonia scholaris |
| title_fullStr | Lipoxygenase inhibition and molecular docking studies of secondary metabolites from the leaves of Alstonia scholaris |
| title_full_unstemmed | Lipoxygenase inhibition and molecular docking studies of secondary metabolites from the leaves of Alstonia scholaris |
| title_short | Lipoxygenase inhibition and molecular docking studies of secondary metabolites from the leaves of Alstonia scholaris |
| title_sort | lipoxygenase inhibition and molecular docking studies of secondary metabolites from the leaves of alstonia scholaris |
| topic | Alstonia scholaris Lipoxygenase Cancer Docking study β-Sitosterol glucoside Scholaricine |
| url | http://www.sciencedirect.com/science/article/pii/S2772417423000080 |
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