Yeast One-Hybrid Screening to Identify Transcription Factors for <i>IbMYB1-4</i> in the Purple-Fleshed Sweet Potato (<i>Ipomoea batatas</i> [L.] Lam.)
IbMYB1 is a transcription factor involved in the biosynthesis of anthocyanin in the purple-fleshed sweet potato. So far, few studies have investigated transcription factors that are upstream of the promoter <i>IbMYB1-4</i>. In this study, a yeast one-hybrid screening aimed at identifying...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-07-01
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| Series: | Current Issues in Molecular Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1467-3045/45/7/364 |
| Summary: | IbMYB1 is a transcription factor involved in the biosynthesis of anthocyanin in the purple-fleshed sweet potato. So far, few studies have investigated transcription factors that are upstream of the promoter <i>IbMYB1-4</i>. In this study, a yeast one-hybrid screening aimed at identifying transcription factors upstream of the promoter <i>IbMYB1-4</i> was performed in the storage roots of the purple-fleshed sweet potato, and IbPDC, IbERF1, and IbPGP19 were identified as upstream binding proteins for the promoter <i>IbMYB1-4</i>. A dual luciferase reporter assay, and yeast one-hybrid assays, were employed to confirm the interaction of these binding proteins with promoters. IbERF1 was found to be an upstream transcription factor for the promoter <i>IbMYB1</i>, and is implicated in the biosynthesis of anthocyanin in the purple-fleshed sweet potato. IbERF1 plays a major role in the biosynthesis of anthocyanin in the purple-fleshed sweet potato. |
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| ISSN: | 1467-3037 1467-3045 |