Yeast One-Hybrid Screening to Identify Transcription Factors for <i>IbMYB1-4</i> in the Purple-Fleshed Sweet Potato (<i>Ipomoea batatas</i> [L.] Lam.)
IbMYB1 is a transcription factor involved in the biosynthesis of anthocyanin in the purple-fleshed sweet potato. So far, few studies have investigated transcription factors that are upstream of the promoter <i>IbMYB1-4</i>. In this study, a yeast one-hybrid screening aimed at identifying...
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MDPI AG
2023-07-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/45/7/364 |
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| author | Danwen Fu Shaohua Yang Rui Liu Feng Gao |
| author_facet | Danwen Fu Shaohua Yang Rui Liu Feng Gao |
| author_sort | Danwen Fu |
| collection | DOAJ |
| description | IbMYB1 is a transcription factor involved in the biosynthesis of anthocyanin in the purple-fleshed sweet potato. So far, few studies have investigated transcription factors that are upstream of the promoter <i>IbMYB1-4</i>. In this study, a yeast one-hybrid screening aimed at identifying transcription factors upstream of the promoter <i>IbMYB1-4</i> was performed in the storage roots of the purple-fleshed sweet potato, and IbPDC, IbERF1, and IbPGP19 were identified as upstream binding proteins for the promoter <i>IbMYB1-4</i>. A dual luciferase reporter assay, and yeast one-hybrid assays, were employed to confirm the interaction of these binding proteins with promoters. IbERF1 was found to be an upstream transcription factor for the promoter <i>IbMYB1</i>, and is implicated in the biosynthesis of anthocyanin in the purple-fleshed sweet potato. IbERF1 plays a major role in the biosynthesis of anthocyanin in the purple-fleshed sweet potato. |
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| language | English |
| last_indexed | 2024-03-11T01:11:10Z |
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| spelling | doaj.art-def1c9e4c5824ce1a3476917a4c3a2162023-11-18T18:51:12ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452023-07-014575765577510.3390/cimb45070364Yeast One-Hybrid Screening to Identify Transcription Factors for <i>IbMYB1-4</i> in the Purple-Fleshed Sweet Potato (<i>Ipomoea batatas</i> [L.] Lam.)Danwen Fu0Shaohua Yang1Rui Liu2Feng Gao3Guangdong Provincial Key Lab of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, ChinaGuangdong Provincial Key Lab of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, ChinaInstitute of Nanfan & Seed Industry, Guangdong Academy of Sciences, Guangzhou 510316, ChinaGuangdong Provincial Key Lab of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, ChinaIbMYB1 is a transcription factor involved in the biosynthesis of anthocyanin in the purple-fleshed sweet potato. So far, few studies have investigated transcription factors that are upstream of the promoter <i>IbMYB1-4</i>. In this study, a yeast one-hybrid screening aimed at identifying transcription factors upstream of the promoter <i>IbMYB1-4</i> was performed in the storage roots of the purple-fleshed sweet potato, and IbPDC, IbERF1, and IbPGP19 were identified as upstream binding proteins for the promoter <i>IbMYB1-4</i>. A dual luciferase reporter assay, and yeast one-hybrid assays, were employed to confirm the interaction of these binding proteins with promoters. IbERF1 was found to be an upstream transcription factor for the promoter <i>IbMYB1</i>, and is implicated in the biosynthesis of anthocyanin in the purple-fleshed sweet potato. IbERF1 plays a major role in the biosynthesis of anthocyanin in the purple-fleshed sweet potato.https://www.mdpi.com/1467-3045/45/7/364anthocyanin biosynthesisIbERF1IbPDCIbPGP19upstream transcription factors |
| spellingShingle | Danwen Fu Shaohua Yang Rui Liu Feng Gao Yeast One-Hybrid Screening to Identify Transcription Factors for <i>IbMYB1-4</i> in the Purple-Fleshed Sweet Potato (<i>Ipomoea batatas</i> [L.] Lam.) Current Issues in Molecular Biology anthocyanin biosynthesis IbERF1 IbPDC IbPGP19 upstream transcription factors |
| title | Yeast One-Hybrid Screening to Identify Transcription Factors for <i>IbMYB1-4</i> in the Purple-Fleshed Sweet Potato (<i>Ipomoea batatas</i> [L.] Lam.) |
| title_full | Yeast One-Hybrid Screening to Identify Transcription Factors for <i>IbMYB1-4</i> in the Purple-Fleshed Sweet Potato (<i>Ipomoea batatas</i> [L.] Lam.) |
| title_fullStr | Yeast One-Hybrid Screening to Identify Transcription Factors for <i>IbMYB1-4</i> in the Purple-Fleshed Sweet Potato (<i>Ipomoea batatas</i> [L.] Lam.) |
| title_full_unstemmed | Yeast One-Hybrid Screening to Identify Transcription Factors for <i>IbMYB1-4</i> in the Purple-Fleshed Sweet Potato (<i>Ipomoea batatas</i> [L.] Lam.) |
| title_short | Yeast One-Hybrid Screening to Identify Transcription Factors for <i>IbMYB1-4</i> in the Purple-Fleshed Sweet Potato (<i>Ipomoea batatas</i> [L.] Lam.) |
| title_sort | yeast one hybrid screening to identify transcription factors for i ibmyb1 4 i in the purple fleshed sweet potato i ipomoea batatas i l lam |
| topic | anthocyanin biosynthesis IbERF1 IbPDC IbPGP19 upstream transcription factors |
| url | https://www.mdpi.com/1467-3045/45/7/364 |
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