Nucleotide-Binding Leucine-Rich Repeat Genes <i>CsRSF1</i> and <i>CsRSF2</i> Are Positive Modulators in the <i>Cucumis sativus</i> Defense Response to <i>Sphaerotheca fuliginea</i>
Cucumber powdery mildew caused by <i>Sphaerotheca fuliginea</i> is a leaf disease that seriously affects cucumber’s yield and quality. This study aimed to report two nucleotide-binding site-leucine-rich repeats (NBS-LRR) genes <i>CsRSF1</i> and <i>CsRSF2</i>, whic...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-04-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/22/8/3986 |
| Summary: | Cucumber powdery mildew caused by <i>Sphaerotheca fuliginea</i> is a leaf disease that seriously affects cucumber’s yield and quality. This study aimed to report two nucleotide-binding site-leucine-rich repeats (NBS-LRR) genes <i>CsRSF1</i> and <i>CsRSF2</i>, which participated in regulating the resistance of cucumber to <i>S. fuliginea</i>. The subcellular localization showed that the CsRSF1 protein was localized in the nucleus, cytoplasm, and cell membrane, while the CsRSF2 protein was localized in the cell membrane and cytoplasm. In addition, the transcript levels of <i>CsRSF1</i> and <i>CsRSF2</i> were different between resistant and susceptible cultivars after treatment with exogenous substances, such as abscisic acid (ABA), methyl jasmonate (MeJA), salicylic acid (SA), ethephon (ETH), gibberellin (GA) and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). The expression analysis showed that the transcript levels of <i>CsRSF1</i> and <i>CsRSF2</i> were correlated with plant defense response against <i>S. fuliginea</i>. Moreover, the silencing of <i>CsRSF1</i> and <i>CsRSF2</i> impaired host resistance to <i>S. fuliginea</i>, but <i>CsRSF1</i> and <i>CsRSF2</i> overexpression improved resistance to <i>S. fuliginea</i> in cucumber. These results showed that <i>CsRSF1</i> and <i>CsRSF2</i> genes positively contributed to the resistance of cucumber to <i>S. fuliginea</i>. At the same time, <i>CsRSF1</i> and <i>CsRSF2</i> genes could also regulate the expression of defense-related genes. The findings of this study might help enhance the resistance of cucumber to <i>S. fuliginea</i>. |
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| ISSN: | 1661-6596 1422-0067 |