Nucleotide-Binding Leucine-Rich Repeat Genes <i>CsRSF1</i> and <i>CsRSF2</i> Are Positive Modulators in the <i>Cucumis sativus</i> Defense Response to <i>Sphaerotheca fuliginea</i>
Cucumber powdery mildew caused by <i>Sphaerotheca fuliginea</i> is a leaf disease that seriously affects cucumber’s yield and quality. This study aimed to report two nucleotide-binding site-leucine-rich repeats (NBS-LRR) genes <i>CsRSF1</i> and <i>CsRSF2</i>, whic...
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MDPI AG
2021-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/8/3986 |
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| author | Xue Wang Qiumin Chen Jingnan Huang Xiangnan Meng Na Cui Yang Yu Haiyan Fan |
| author_facet | Xue Wang Qiumin Chen Jingnan Huang Xiangnan Meng Na Cui Yang Yu Haiyan Fan |
| author_sort | Xue Wang |
| collection | DOAJ |
| description | Cucumber powdery mildew caused by <i>Sphaerotheca fuliginea</i> is a leaf disease that seriously affects cucumber’s yield and quality. This study aimed to report two nucleotide-binding site-leucine-rich repeats (NBS-LRR) genes <i>CsRSF1</i> and <i>CsRSF2</i>, which participated in regulating the resistance of cucumber to <i>S. fuliginea</i>. The subcellular localization showed that the CsRSF1 protein was localized in the nucleus, cytoplasm, and cell membrane, while the CsRSF2 protein was localized in the cell membrane and cytoplasm. In addition, the transcript levels of <i>CsRSF1</i> and <i>CsRSF2</i> were different between resistant and susceptible cultivars after treatment with exogenous substances, such as abscisic acid (ABA), methyl jasmonate (MeJA), salicylic acid (SA), ethephon (ETH), gibberellin (GA) and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). The expression analysis showed that the transcript levels of <i>CsRSF1</i> and <i>CsRSF2</i> were correlated with plant defense response against <i>S. fuliginea</i>. Moreover, the silencing of <i>CsRSF1</i> and <i>CsRSF2</i> impaired host resistance to <i>S. fuliginea</i>, but <i>CsRSF1</i> and <i>CsRSF2</i> overexpression improved resistance to <i>S. fuliginea</i> in cucumber. These results showed that <i>CsRSF1</i> and <i>CsRSF2</i> genes positively contributed to the resistance of cucumber to <i>S. fuliginea</i>. At the same time, <i>CsRSF1</i> and <i>CsRSF2</i> genes could also regulate the expression of defense-related genes. The findings of this study might help enhance the resistance of cucumber to <i>S. fuliginea</i>. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T12:23:37Z |
| publishDate | 2021-04-01 |
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| spelling | doaj.art-def2bed2f7bd4480bcd385dccb881e492023-11-21T15:18:09ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-04-01228398610.3390/ijms22083986Nucleotide-Binding Leucine-Rich Repeat Genes <i>CsRSF1</i> and <i>CsRSF2</i> Are Positive Modulators in the <i>Cucumis sativus</i> Defense Response to <i>Sphaerotheca fuliginea</i>Xue Wang0Qiumin Chen1Jingnan Huang2Xiangnan Meng3Na Cui4Yang Yu5Haiyan Fan6College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, ChinaCucumber powdery mildew caused by <i>Sphaerotheca fuliginea</i> is a leaf disease that seriously affects cucumber’s yield and quality. This study aimed to report two nucleotide-binding site-leucine-rich repeats (NBS-LRR) genes <i>CsRSF1</i> and <i>CsRSF2</i>, which participated in regulating the resistance of cucumber to <i>S. fuliginea</i>. The subcellular localization showed that the CsRSF1 protein was localized in the nucleus, cytoplasm, and cell membrane, while the CsRSF2 protein was localized in the cell membrane and cytoplasm. In addition, the transcript levels of <i>CsRSF1</i> and <i>CsRSF2</i> were different between resistant and susceptible cultivars after treatment with exogenous substances, such as abscisic acid (ABA), methyl jasmonate (MeJA), salicylic acid (SA), ethephon (ETH), gibberellin (GA) and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). The expression analysis showed that the transcript levels of <i>CsRSF1</i> and <i>CsRSF2</i> were correlated with plant defense response against <i>S. fuliginea</i>. Moreover, the silencing of <i>CsRSF1</i> and <i>CsRSF2</i> impaired host resistance to <i>S. fuliginea</i>, but <i>CsRSF1</i> and <i>CsRSF2</i> overexpression improved resistance to <i>S. fuliginea</i> in cucumber. These results showed that <i>CsRSF1</i> and <i>CsRSF2</i> genes positively contributed to the resistance of cucumber to <i>S. fuliginea</i>. At the same time, <i>CsRSF1</i> and <i>CsRSF2</i> genes could also regulate the expression of defense-related genes. The findings of this study might help enhance the resistance of cucumber to <i>S. fuliginea</i>.https://www.mdpi.com/1422-0067/22/8/3986<i>Cucumis sativus</i>NBS-LRRexpression analysis<i>Sphaerotheca fuliginea</i>transient genetic transformation |
| spellingShingle | Xue Wang Qiumin Chen Jingnan Huang Xiangnan Meng Na Cui Yang Yu Haiyan Fan Nucleotide-Binding Leucine-Rich Repeat Genes <i>CsRSF1</i> and <i>CsRSF2</i> Are Positive Modulators in the <i>Cucumis sativus</i> Defense Response to <i>Sphaerotheca fuliginea</i> International Journal of Molecular Sciences <i>Cucumis sativus</i> NBS-LRR expression analysis <i>Sphaerotheca fuliginea</i> transient genetic transformation |
| title | Nucleotide-Binding Leucine-Rich Repeat Genes <i>CsRSF1</i> and <i>CsRSF2</i> Are Positive Modulators in the <i>Cucumis sativus</i> Defense Response to <i>Sphaerotheca fuliginea</i> |
| title_full | Nucleotide-Binding Leucine-Rich Repeat Genes <i>CsRSF1</i> and <i>CsRSF2</i> Are Positive Modulators in the <i>Cucumis sativus</i> Defense Response to <i>Sphaerotheca fuliginea</i> |
| title_fullStr | Nucleotide-Binding Leucine-Rich Repeat Genes <i>CsRSF1</i> and <i>CsRSF2</i> Are Positive Modulators in the <i>Cucumis sativus</i> Defense Response to <i>Sphaerotheca fuliginea</i> |
| title_full_unstemmed | Nucleotide-Binding Leucine-Rich Repeat Genes <i>CsRSF1</i> and <i>CsRSF2</i> Are Positive Modulators in the <i>Cucumis sativus</i> Defense Response to <i>Sphaerotheca fuliginea</i> |
| title_short | Nucleotide-Binding Leucine-Rich Repeat Genes <i>CsRSF1</i> and <i>CsRSF2</i> Are Positive Modulators in the <i>Cucumis sativus</i> Defense Response to <i>Sphaerotheca fuliginea</i> |
| title_sort | nucleotide binding leucine rich repeat genes i csrsf1 i and i csrsf2 i are positive modulators in the i cucumis sativus i defense response to i sphaerotheca fuliginea i |
| topic | <i>Cucumis sativus</i> NBS-LRR expression analysis <i>Sphaerotheca fuliginea</i> transient genetic transformation |
| url | https://www.mdpi.com/1422-0067/22/8/3986 |
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