Quantification of oxygen consumption in head and neck cancer using fluorescent sensor foil technology
IntroductionHead and neck squamous cell carcinoma (HNSCC) patients suffer from frequent local recurrences that negatively impact on prognosis. Hence, distinguishing tumor and normal tissue is of clinical importance as it may improve the detection of residual tumor tissue in surgical resection margin...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2024.1002798/full |
| _version_ | 1797320927318376448 |
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| author | Magdalena Stocker Alexandra Blancke Soares Gregor Liebsch Robert J. Meier Martin Canis Olivier Gires Frank Haubner |
| author_facet | Magdalena Stocker Alexandra Blancke Soares Gregor Liebsch Robert J. Meier Martin Canis Olivier Gires Frank Haubner |
| author_sort | Magdalena Stocker |
| collection | DOAJ |
| description | IntroductionHead and neck squamous cell carcinoma (HNSCC) patients suffer from frequent local recurrences that negatively impact on prognosis. Hence, distinguishing tumor and normal tissue is of clinical importance as it may improve the detection of residual tumor tissue in surgical resection margins and during imaging-based surgery planning. Differences in O2 consumption (OC) can be used to this aim, as they provide options for improved surgical, image-guided approaches.MethodsIn the present study, the potential of a fluorescent sensor foil-based technology to quantify OC in HNSCC was evaluated in an in vitro 3D model and in situ in patients. ResultsIn vitro measurements of OC using hypopharyngeal and esophageal cell lines allowed a specific detection of tumor cell spheroids embedded together with cancer-associated fibroblasts in type I collagen extracellular matrix down to a diameter of 440 µm. Pre-surgery in situ measurements were conducted with a handheld recording device and sensor foils with an oxygen permeable membrane and immobilized O2-reactive fluorescent dyes. Lateral tongue carcinoma and carcinoma of the floor of the mouth were chosen for analysis owing to their facilitated accessibility. OC was evaluated over a time span of 60 seconds and was significantly higher in tumor tissue compared to healthy mucosa in the vicinity of the tumor.DiscussionHence, OC quantification using fluorescent sensor foil-based technology is a relevant parameter for the differentiation of tumor tissue of the head and neck region and may support surgery planning. |
| first_indexed | 2024-03-08T04:50:06Z |
| format | Article |
| id | doaj.art-def4c067729e4e8abf3f24c6288cdc54 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-03-08T04:50:06Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-def4c067729e4e8abf3f24c6288cdc542024-02-08T05:17:48ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2024-02-011410.3389/fonc.2024.10027981002798Quantification of oxygen consumption in head and neck cancer using fluorescent sensor foil technologyMagdalena Stocker0Alexandra Blancke Soares1Gregor Liebsch2Robert J. Meier3Martin Canis4Olivier Gires5Frank Haubner6Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital, Ludwig Maximilians University (LMU) Munich, Munich, GermanyDepartment of Otorhinolaryngology, Head and Neck Surgery, University Hospital, Ludwig Maximilians University (LMU) Munich, Munich, GermanyPreSens Precision Sensing GmbH, Imaging Solutions, Regensburg, GermanyPreSens Precision Sensing GmbH, Imaging Solutions, Regensburg, GermanyDepartment of Otorhinolaryngology, Head and Neck Surgery, University Hospital, Ludwig Maximilians University (LMU) Munich, Munich, GermanyDepartment of Otorhinolaryngology, Head and Neck Surgery, University Hospital, Ludwig Maximilians University (LMU) Munich, Munich, GermanyDepartment of Otorhinolaryngology, Head and Neck Surgery, University Hospital, Ludwig Maximilians University (LMU) Munich, Munich, GermanyIntroductionHead and neck squamous cell carcinoma (HNSCC) patients suffer from frequent local recurrences that negatively impact on prognosis. Hence, distinguishing tumor and normal tissue is of clinical importance as it may improve the detection of residual tumor tissue in surgical resection margins and during imaging-based surgery planning. Differences in O2 consumption (OC) can be used to this aim, as they provide options for improved surgical, image-guided approaches.MethodsIn the present study, the potential of a fluorescent sensor foil-based technology to quantify OC in HNSCC was evaluated in an in vitro 3D model and in situ in patients. ResultsIn vitro measurements of OC using hypopharyngeal and esophageal cell lines allowed a specific detection of tumor cell spheroids embedded together with cancer-associated fibroblasts in type I collagen extracellular matrix down to a diameter of 440 µm. Pre-surgery in situ measurements were conducted with a handheld recording device and sensor foils with an oxygen permeable membrane and immobilized O2-reactive fluorescent dyes. Lateral tongue carcinoma and carcinoma of the floor of the mouth were chosen for analysis owing to their facilitated accessibility. OC was evaluated over a time span of 60 seconds and was significantly higher in tumor tissue compared to healthy mucosa in the vicinity of the tumor.DiscussionHence, OC quantification using fluorescent sensor foil-based technology is a relevant parameter for the differentiation of tumor tissue of the head and neck region and may support surgery planning.https://www.frontiersin.org/articles/10.3389/fonc.2024.1002798/fulltumor hypoxiaHNSCCtumor imaginghead and neck canceroxygen consumption |
| spellingShingle | Magdalena Stocker Alexandra Blancke Soares Gregor Liebsch Robert J. Meier Martin Canis Olivier Gires Frank Haubner Quantification of oxygen consumption in head and neck cancer using fluorescent sensor foil technology Frontiers in Oncology tumor hypoxia HNSCC tumor imaging head and neck cancer oxygen consumption |
| title | Quantification of oxygen consumption in head and neck cancer using fluorescent sensor foil technology |
| title_full | Quantification of oxygen consumption in head and neck cancer using fluorescent sensor foil technology |
| title_fullStr | Quantification of oxygen consumption in head and neck cancer using fluorescent sensor foil technology |
| title_full_unstemmed | Quantification of oxygen consumption in head and neck cancer using fluorescent sensor foil technology |
| title_short | Quantification of oxygen consumption in head and neck cancer using fluorescent sensor foil technology |
| title_sort | quantification of oxygen consumption in head and neck cancer using fluorescent sensor foil technology |
| topic | tumor hypoxia HNSCC tumor imaging head and neck cancer oxygen consumption |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2024.1002798/full |
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