Gut microbial profiles and the role in lipid metabolism in Shaziling pigs
Shaziling pig, a Chinese indigenous breed, has been classified as a fatty pig model. However, the gut microbial development and role in lipid metabolism in Shaziling pigs has been rarely reported. Here, we compared the lipid metabolic and microbial profiles at 30, 60, 90, 150, 210, and 300 d of age...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2022-06-01
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| Series: | Animal Nutrition |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405654522000154 |
| _version_ | 1811325504027885568 |
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| author | Jie Ma Yehui Duan Rui Li Xiaoxiao Liang Tiejun Li Xingguo Huang Yulong Yin Jie Yin |
| author_facet | Jie Ma Yehui Duan Rui Li Xiaoxiao Liang Tiejun Li Xingguo Huang Yulong Yin Jie Yin |
| author_sort | Jie Ma |
| collection | DOAJ |
| description | Shaziling pig, a Chinese indigenous breed, has been classified as a fatty pig model. However, the gut microbial development and role in lipid metabolism in Shaziling pigs has been rarely reported. Here, we compared the lipid metabolic and microbial profiles at 30, 60, 90, 150, 210, and 300 d of age between Shaziling and Yorkshire pigs. Predictably, there were marked differences in the liver lipids (i.e., cholesterol, glucose, and low-density lipoprotein) and the lipid related expressions (i.e., SREBP1/2, LXRα/β, DGAT1/2, and FABP1-3) between Shaziling and Yorkshire pigs. Bacteria sequencing in the ileal digesta and mucosa showed that Shaziling pigs had a higher α-diversity and higher abundances of probiotics, such as Lactobacillus johnsonii, Lactobacillus amylovorus, and Clostridium butyricum. Thirty-five differentiated metabolites were further identified in the mucosa between Shaziling and Yorkshire pigs, which were enriched in the carbohydrate, protein, glucose and amino acid metabolism and bile acid biosynthesis. Furthermore, 7 differentiated microbial species were markedly correlated with metabolites, indicating the role of gut microbiota in the host metabolism. Next, the role of differentiated L. johnsonii in lipid metabolism was validated in Duroc × Landrace × Yorkshire (DLY) pigs and the results showed that L. johnsonii mono-colonization promoted lipid deposition and metabolism by altering gut microbiota (i.e., Megasphaera elsdenii and L. johnsonii) and DGAT1/DGAT2/CD36-PPARγ gene expressions. In conclusion, Shaziling pigs exhibited different metabolic and microbial profiles compared with Yorkshire pigs, which might have contributed to the diverse metabolic phenotypes, and the significant enrichment of L. johnsonii in Shaziling pigs promoted lipid metabolism and obesity of DLY pigs, which provided a novel idea to improve the fat content of lean pigs. |
| first_indexed | 2024-04-13T14:34:21Z |
| format | Article |
| id | doaj.art-def93ce646aa4435beafc9e7f7a57b38 |
| institution | Directory Open Access Journal |
| issn | 2405-6545 |
| language | English |
| last_indexed | 2024-04-13T14:34:21Z |
| publishDate | 2022-06-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Animal Nutrition |
| spelling | doaj.art-def93ce646aa4435beafc9e7f7a57b382022-12-22T02:43:06ZengKeAi Communications Co., Ltd.Animal Nutrition2405-65452022-06-019345356Gut microbial profiles and the role in lipid metabolism in Shaziling pigsJie Ma0Yehui Duan1Rui Li2Xiaoxiao Liang3Tiejun Li4Xingguo Huang5Yulong Yin6Jie Yin7Animal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha, 410128, ChinaInstitute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, ChinaInstitute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, ChinaHenan Ground Biological Science & Technology Co., Ltd., ChinaInstitute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, ChinaAnimal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha, 410128, ChinaAnimal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha, 410128, China; Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, ChinaAnimal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha, 410128, China; Corresponding author.Shaziling pig, a Chinese indigenous breed, has been classified as a fatty pig model. However, the gut microbial development and role in lipid metabolism in Shaziling pigs has been rarely reported. Here, we compared the lipid metabolic and microbial profiles at 30, 60, 90, 150, 210, and 300 d of age between Shaziling and Yorkshire pigs. Predictably, there were marked differences in the liver lipids (i.e., cholesterol, glucose, and low-density lipoprotein) and the lipid related expressions (i.e., SREBP1/2, LXRα/β, DGAT1/2, and FABP1-3) between Shaziling and Yorkshire pigs. Bacteria sequencing in the ileal digesta and mucosa showed that Shaziling pigs had a higher α-diversity and higher abundances of probiotics, such as Lactobacillus johnsonii, Lactobacillus amylovorus, and Clostridium butyricum. Thirty-five differentiated metabolites were further identified in the mucosa between Shaziling and Yorkshire pigs, which were enriched in the carbohydrate, protein, glucose and amino acid metabolism and bile acid biosynthesis. Furthermore, 7 differentiated microbial species were markedly correlated with metabolites, indicating the role of gut microbiota in the host metabolism. Next, the role of differentiated L. johnsonii in lipid metabolism was validated in Duroc × Landrace × Yorkshire (DLY) pigs and the results showed that L. johnsonii mono-colonization promoted lipid deposition and metabolism by altering gut microbiota (i.e., Megasphaera elsdenii and L. johnsonii) and DGAT1/DGAT2/CD36-PPARγ gene expressions. In conclusion, Shaziling pigs exhibited different metabolic and microbial profiles compared with Yorkshire pigs, which might have contributed to the diverse metabolic phenotypes, and the significant enrichment of L. johnsonii in Shaziling pigs promoted lipid metabolism and obesity of DLY pigs, which provided a novel idea to improve the fat content of lean pigs.http://www.sciencedirect.com/science/article/pii/S2405654522000154Shaziling pigGut microbiotaLipid metabolismLactobacillus johnsoniiFatty acid |
| spellingShingle | Jie Ma Yehui Duan Rui Li Xiaoxiao Liang Tiejun Li Xingguo Huang Yulong Yin Jie Yin Gut microbial profiles and the role in lipid metabolism in Shaziling pigs Animal Nutrition Shaziling pig Gut microbiota Lipid metabolism Lactobacillus johnsonii Fatty acid |
| title | Gut microbial profiles and the role in lipid metabolism in Shaziling pigs |
| title_full | Gut microbial profiles and the role in lipid metabolism in Shaziling pigs |
| title_fullStr | Gut microbial profiles and the role in lipid metabolism in Shaziling pigs |
| title_full_unstemmed | Gut microbial profiles and the role in lipid metabolism in Shaziling pigs |
| title_short | Gut microbial profiles and the role in lipid metabolism in Shaziling pigs |
| title_sort | gut microbial profiles and the role in lipid metabolism in shaziling pigs |
| topic | Shaziling pig Gut microbiota Lipid metabolism Lactobacillus johnsonii Fatty acid |
| url | http://www.sciencedirect.com/science/article/pii/S2405654522000154 |
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