Expression Analysis of NF-κB-Related lncRNAs in Parkinson’s Disease
Parkinson’s disease (PD) has been shown to affect approximately 1% of the persons aged more than 65 years. This multifactorial disorder has been associated with abnormal function of NF-κB signals. In this research, we have evaluated expressions of NF-κB-related long non-coding RNAs in the circulatio...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-10-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2021.755246/full |
| _version_ | 1818591411332710400 |
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| author | Soudeh Ghafouri-Fard Mahdi Gholipour Atefe Abak Mehrdokht Mazdeh Mohammad Taheri Arezou Sayad |
| author_facet | Soudeh Ghafouri-Fard Mahdi Gholipour Atefe Abak Mehrdokht Mazdeh Mohammad Taheri Arezou Sayad |
| author_sort | Soudeh Ghafouri-Fard |
| collection | DOAJ |
| description | Parkinson’s disease (PD) has been shown to affect approximately 1% of the persons aged more than 65 years. This multifactorial disorder has been associated with abnormal function of NF-κB signals. In this research, we have evaluated expressions of NF-κB-related long non-coding RNAs in the circulation of PD patients compared with healthy controls. Expression of PACER was lower in total PD patients compared with healthy persons (Ratio of mean expressions (RME)=0.32, P value<0.001). This pattern was also evident among males (RME=0.25, P value<0.001). Expression of DILC was higher in total PD patients (RME=4.07, P value<0.001), and in both sex-based subgroups (RME=3.77, P value=0.01 and RME=4.25, P value<0.001, for females and males, respectively). Similarly, CEBPA was significantly over-expressed in total PD patients (RME=14.76, P value<0.001), and in both sex-based subgroups (RME=12.42, P value<0.001 and RME=15.80, P value<0.001, for females and males, respectively). ATG5 had a similar expression pattern (RME=2.6, P value=1E-08, RME=1.73, P value=0.03 and RME=3.09, P value=1E-07, for total cases, females and males, respectively). H19 was up-regulated in total cases and male cases compared with corresponding controls (RME=2.19, P value<0.001, RME=2.68, P value=0.01, respectively). Finally, HNFA1-AS was down-regulated in all comparisons (RME=0.10, P value=2E-06, RME=0.08, P value<0.001 and RME=0.12, P value<0.001, for total cases, females and males, respectively). Among PD patients, expressions of NKILA and ADINR were robustly correlated with each other (r=0.75, P value=2.40E-10). In addition, expression levels of DICER1-AS were significantly correlated with those of ADINR, PACER and H19 in these patients (r=0.73, P value=1.76E-9; r=0.72, P value=5.15E-09 and r=0.72, P value=3.09E-09, respectively). Correlation analyses among healthy controls revealed robust correlations between CHAST and CEBPA (r=0.84, P value=3.09E-09), NKILA and ADINR (r=0.80, P value=4.24E-12) as well as between DILC and CHAST (r=0.76, P value=1.70E-10). CEBPA had the best parameters among all assessed genes (AUC=0.96, Sensitivity=0.90 and specificity=0.97). DILC and ATG5 were the most appropriate markers after CEBPA with AUC values of 0.82 and 0.80, respectively. Most notably, combination of all genes improved AUC, sensitivity and specificity parameters to 1, 0.97 and 0.99, respectively. Cumulatively, the current study provides evidence for participation of NF-κB-related lncRNAs in the pathoetiology of PD. |
| first_indexed | 2024-12-16T10:12:01Z |
| format | Article |
| id | doaj.art-def9e3a3ce7e44cd9751502a415390ae |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-16T10:12:01Z |
| publishDate | 2021-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-def9e3a3ce7e44cd9751502a415390ae2022-12-21T22:35:34ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-10-011210.3389/fimmu.2021.755246755246Expression Analysis of NF-κB-Related lncRNAs in Parkinson’s DiseaseSoudeh Ghafouri-Fard0Mahdi Gholipour1Atefe Abak2Mehrdokht Mazdeh3Mohammad Taheri4Arezou Sayad5Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranPhytochemistry Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IranMen’s Health and Reproductive Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IranNeurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, IranSkull Base Research Center, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, IranUrology and Nephrology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IranParkinson’s disease (PD) has been shown to affect approximately 1% of the persons aged more than 65 years. This multifactorial disorder has been associated with abnormal function of NF-κB signals. In this research, we have evaluated expressions of NF-κB-related long non-coding RNAs in the circulation of PD patients compared with healthy controls. Expression of PACER was lower in total PD patients compared with healthy persons (Ratio of mean expressions (RME)=0.32, P value<0.001). This pattern was also evident among males (RME=0.25, P value<0.001). Expression of DILC was higher in total PD patients (RME=4.07, P value<0.001), and in both sex-based subgroups (RME=3.77, P value=0.01 and RME=4.25, P value<0.001, for females and males, respectively). Similarly, CEBPA was significantly over-expressed in total PD patients (RME=14.76, P value<0.001), and in both sex-based subgroups (RME=12.42, P value<0.001 and RME=15.80, P value<0.001, for females and males, respectively). ATG5 had a similar expression pattern (RME=2.6, P value=1E-08, RME=1.73, P value=0.03 and RME=3.09, P value=1E-07, for total cases, females and males, respectively). H19 was up-regulated in total cases and male cases compared with corresponding controls (RME=2.19, P value<0.001, RME=2.68, P value=0.01, respectively). Finally, HNFA1-AS was down-regulated in all comparisons (RME=0.10, P value=2E-06, RME=0.08, P value<0.001 and RME=0.12, P value<0.001, for total cases, females and males, respectively). Among PD patients, expressions of NKILA and ADINR were robustly correlated with each other (r=0.75, P value=2.40E-10). In addition, expression levels of DICER1-AS were significantly correlated with those of ADINR, PACER and H19 in these patients (r=0.73, P value=1.76E-9; r=0.72, P value=5.15E-09 and r=0.72, P value=3.09E-09, respectively). Correlation analyses among healthy controls revealed robust correlations between CHAST and CEBPA (r=0.84, P value=3.09E-09), NKILA and ADINR (r=0.80, P value=4.24E-12) as well as between DILC and CHAST (r=0.76, P value=1.70E-10). CEBPA had the best parameters among all assessed genes (AUC=0.96, Sensitivity=0.90 and specificity=0.97). DILC and ATG5 were the most appropriate markers after CEBPA with AUC values of 0.82 and 0.80, respectively. Most notably, combination of all genes improved AUC, sensitivity and specificity parameters to 1, 0.97 and 0.99, respectively. Cumulatively, the current study provides evidence for participation of NF-κB-related lncRNAs in the pathoetiology of PD.https://www.frontiersin.org/articles/10.3389/fimmu.2021.755246/fullParkinson’s diseaselncRNANF-κBexpressionbiomarker |
| spellingShingle | Soudeh Ghafouri-Fard Mahdi Gholipour Atefe Abak Mehrdokht Mazdeh Mohammad Taheri Arezou Sayad Expression Analysis of NF-κB-Related lncRNAs in Parkinson’s Disease Frontiers in Immunology Parkinson’s disease lncRNA NF-κB expression biomarker |
| title | Expression Analysis of NF-κB-Related lncRNAs in Parkinson’s Disease |
| title_full | Expression Analysis of NF-κB-Related lncRNAs in Parkinson’s Disease |
| title_fullStr | Expression Analysis of NF-κB-Related lncRNAs in Parkinson’s Disease |
| title_full_unstemmed | Expression Analysis of NF-κB-Related lncRNAs in Parkinson’s Disease |
| title_short | Expression Analysis of NF-κB-Related lncRNAs in Parkinson’s Disease |
| title_sort | expression analysis of nf κb related lncrnas in parkinson s disease |
| topic | Parkinson’s disease lncRNA NF-κB expression biomarker |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2021.755246/full |
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