Kinetics and Distribution of Zearalenone-14-Glucoside and Its Metabolite Zearalenone in Rat, Determined by a Reliable HPLC-MS/MS Method
A reliable high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was established for the simultaneous detection of zearalenone-14-glucoside (ZEN-14G) and its metabolite, zearalenone (ZEN), in the plasma, urine, and various tissues of rats. The performance of the develop...
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MDPI AG
2023-04-01
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| Online Access: | https://www.mdpi.com/2076-3417/13/8/4990 |
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| author | Yaling Cai Zhiqi Zhang Fang Dong Zefeng Ma Kai Fan Zheng Han Zhizhong Li Zhihui Zhao |
| author_facet | Yaling Cai Zhiqi Zhang Fang Dong Zefeng Ma Kai Fan Zheng Han Zhizhong Li Zhihui Zhao |
| author_sort | Yaling Cai |
| collection | DOAJ |
| description | A reliable high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was established for the simultaneous detection of zearalenone-14-glucoside (ZEN-14G) and its metabolite, zearalenone (ZEN), in the plasma, urine, and various tissues of rats. The performance of the developed method was validated by determining the selectivity, linearity (<i>R</i><sup>2</sup> > 0.99), sensitivity (lower limit of quantification, 0.1–1 μg/L), recovery (80.7 ± 3.0–112.3 ± 3.1%), precision (0.6–16.5%), and stability (81.7 ± 1.7–104.1 ± 3.9%). Through use of the methodological advances, the subsequent kinetics and distribution after administration of ZEN-14G by gavage were thoroughly investigated. ZEN-14G and ZEN exhibited similar trends in the plasma, and reached their peak concentrations at 10 min and then rapidly decreased. ZEN-14G could be quantified in the stomach, small intestine, and large intestine 24 h after administration, while ZEN was detectable in all tested tissues. Interestingly, ZEN-14G (7.6 ± 3.0 μg/L) and ZEN (977.5 ± 98.0 μg/L) were also detected in the urine 24 h after administration, indicating that ZEN-14G was prone to be slowly and continuously hydrolyzed into ZEN to be absorbed into the plasma and distributed to various tissues, thus leading to a cumulative exposure. Continuous attention should be paid to the co-exposure of ZEN and ZEN-14G, which might pose additional health risks to humans and animals. |
| first_indexed | 2024-03-11T05:16:22Z |
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| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-11T05:16:22Z |
| publishDate | 2023-04-01 |
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| series | Applied Sciences |
| spelling | doaj.art-df006d154e4247868ba0383368c1a1d82023-11-17T18:12:07ZengMDPI AGApplied Sciences2076-34172023-04-01138499010.3390/app13084990Kinetics and Distribution of Zearalenone-14-Glucoside and Its Metabolite Zearalenone in Rat, Determined by a Reliable HPLC-MS/MS MethodYaling Cai0Zhiqi Zhang1Fang Dong2Zefeng Ma3Kai Fan4Zheng Han5Zhizhong Li6Zhihui Zhao7School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, ChinaInstitute for Agro-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaInstitute for Agro-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaInstitute for Agro-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaInstitute for Agro-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaInstitute for Agro-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaSchool of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, ChinaInstitute for Agro-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaA reliable high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was established for the simultaneous detection of zearalenone-14-glucoside (ZEN-14G) and its metabolite, zearalenone (ZEN), in the plasma, urine, and various tissues of rats. The performance of the developed method was validated by determining the selectivity, linearity (<i>R</i><sup>2</sup> > 0.99), sensitivity (lower limit of quantification, 0.1–1 μg/L), recovery (80.7 ± 3.0–112.3 ± 3.1%), precision (0.6–16.5%), and stability (81.7 ± 1.7–104.1 ± 3.9%). Through use of the methodological advances, the subsequent kinetics and distribution after administration of ZEN-14G by gavage were thoroughly investigated. ZEN-14G and ZEN exhibited similar trends in the plasma, and reached their peak concentrations at 10 min and then rapidly decreased. ZEN-14G could be quantified in the stomach, small intestine, and large intestine 24 h after administration, while ZEN was detectable in all tested tissues. Interestingly, ZEN-14G (7.6 ± 3.0 μg/L) and ZEN (977.5 ± 98.0 μg/L) were also detected in the urine 24 h after administration, indicating that ZEN-14G was prone to be slowly and continuously hydrolyzed into ZEN to be absorbed into the plasma and distributed to various tissues, thus leading to a cumulative exposure. Continuous attention should be paid to the co-exposure of ZEN and ZEN-14G, which might pose additional health risks to humans and animals.https://www.mdpi.com/2076-3417/13/8/4990zearalenone-14-glucosidezearalenoneHPLC-MS/MSrat plasmaurinetissues |
| spellingShingle | Yaling Cai Zhiqi Zhang Fang Dong Zefeng Ma Kai Fan Zheng Han Zhizhong Li Zhihui Zhao Kinetics and Distribution of Zearalenone-14-Glucoside and Its Metabolite Zearalenone in Rat, Determined by a Reliable HPLC-MS/MS Method Applied Sciences zearalenone-14-glucoside zearalenone HPLC-MS/MS rat plasma urine tissues |
| title | Kinetics and Distribution of Zearalenone-14-Glucoside and Its Metabolite Zearalenone in Rat, Determined by a Reliable HPLC-MS/MS Method |
| title_full | Kinetics and Distribution of Zearalenone-14-Glucoside and Its Metabolite Zearalenone in Rat, Determined by a Reliable HPLC-MS/MS Method |
| title_fullStr | Kinetics and Distribution of Zearalenone-14-Glucoside and Its Metabolite Zearalenone in Rat, Determined by a Reliable HPLC-MS/MS Method |
| title_full_unstemmed | Kinetics and Distribution of Zearalenone-14-Glucoside and Its Metabolite Zearalenone in Rat, Determined by a Reliable HPLC-MS/MS Method |
| title_short | Kinetics and Distribution of Zearalenone-14-Glucoside and Its Metabolite Zearalenone in Rat, Determined by a Reliable HPLC-MS/MS Method |
| title_sort | kinetics and distribution of zearalenone 14 glucoside and its metabolite zearalenone in rat determined by a reliable hplc ms ms method |
| topic | zearalenone-14-glucoside zearalenone HPLC-MS/MS rat plasma urine tissues |
| url | https://www.mdpi.com/2076-3417/13/8/4990 |
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