Cardiac and renal effects of liver cirrhosis in a growing animal model

ABSTRACT Purpose: To assess the biochemical, histological, histomorphometric and molecular effects of biliary duct ligation (BDL) induced liver cirrhosis in the heart and kidneys. Methods: Thirty-two weaning rats (21 days old, 50-70 g) underwent BDL and were divided in four groups (euthanasia afte...

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Main Authors: Ana Cristina Aoun Tannuri, Leiliane Somoggi Chavez, Juliana Xavier Guimarães, Josiane de Oliveira Gonçalves, Suellen Serafini, Gabriela Carvalho de Souza, Denise Maria Avancini Costa Malheiros, Vitor Ribeiro Paes, Uenis Tannuri
Format: Article
Language:English
Published: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2021-10-01
Series:Acta Cirúrgica Brasileira
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502021000800204&tlng=en
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author Ana Cristina Aoun Tannuri
Leiliane Somoggi Chavez
Juliana Xavier Guimarães
Josiane de Oliveira Gonçalves
Suellen Serafini
Gabriela Carvalho de Souza
Denise Maria Avancini Costa Malheiros
Vitor Ribeiro Paes
Uenis Tannuri
author_facet Ana Cristina Aoun Tannuri
Leiliane Somoggi Chavez
Juliana Xavier Guimarães
Josiane de Oliveira Gonçalves
Suellen Serafini
Gabriela Carvalho de Souza
Denise Maria Avancini Costa Malheiros
Vitor Ribeiro Paes
Uenis Tannuri
author_sort Ana Cristina Aoun Tannuri
collection DOAJ
description ABSTRACT Purpose: To assess the biochemical, histological, histomorphometric and molecular effects of biliary duct ligation (BDL) induced liver cirrhosis in the heart and kidneys. Methods: Thirty-two weaning rats (21 days old, 50-70 g) underwent BDL and were divided in four groups (euthanasia after two, four, six, and eight weeks, respectively) and compared to control groups. Results: The animals’ hearts of group 3 were bigger than those of the control group (p=0.042), including thinner right ventricle wall, decreased internal diameter of ventricles, and increased perivascular collagen deposition in left ventricle, as well as increased interstitial collagen in right ventricle after six weeks. In the kidneys of groups 3 and 4, bilirubin impregnation in the tubules, hydropic degeneration, loss of nuclei and lack of plasmatic membrane limits were noted. Nitric oxide synthase (NOS) gene expressions were higher in group 1 (p=0.008), and endothelial nitric oxide synthase (eNOS) gene expressions were elevated in all experimental groups (p=0.008, p=0.001, p=0.022, and p=0.013, respectively). In the heart, a decreased expression of eNOS in group 1 (p=0.04) was observed. Conclusions: Liver cirrhosis leads to histological and histomorphometric alterations in the heart and kidneys, with changes in the NOS and eNOS gene expressions, that may suggest a role in the associated myocardial and renal manifestations.
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spelling doaj.art-df0ffd1941a44140a103482663f9895d2022-12-21T18:41:55ZengSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira0102-86502021-10-0136810.1590/acb360806Cardiac and renal effects of liver cirrhosis in a growing animal modelAna Cristina Aoun Tannurihttps://orcid.org/0000-0002-5481-032XLeiliane Somoggi Chavezhttps://orcid.org/0000-0003-2577-3598Juliana Xavier Guimarãeshttps://orcid.org/0000-0001-9434-6472Josiane de Oliveira Gonçalveshttps://orcid.org/0000-0002-5402-6455Suellen Serafinihttps://orcid.org/0000-0001-5853-5743Gabriela Carvalho de Souzahttps://orcid.org/0000-0003-1155-7464Denise Maria Avancini Costa Malheiroshttps://orcid.org/0000-0002-0409-816XVitor Ribeiro Paeshttps://orcid.org/0000-0001-8752-5624Uenis Tannurihttps://orcid.org/0000-0002-3855-3298ABSTRACT Purpose: To assess the biochemical, histological, histomorphometric and molecular effects of biliary duct ligation (BDL) induced liver cirrhosis in the heart and kidneys. Methods: Thirty-two weaning rats (21 days old, 50-70 g) underwent BDL and were divided in four groups (euthanasia after two, four, six, and eight weeks, respectively) and compared to control groups. Results: The animals’ hearts of group 3 were bigger than those of the control group (p=0.042), including thinner right ventricle wall, decreased internal diameter of ventricles, and increased perivascular collagen deposition in left ventricle, as well as increased interstitial collagen in right ventricle after six weeks. In the kidneys of groups 3 and 4, bilirubin impregnation in the tubules, hydropic degeneration, loss of nuclei and lack of plasmatic membrane limits were noted. Nitric oxide synthase (NOS) gene expressions were higher in group 1 (p=0.008), and endothelial nitric oxide synthase (eNOS) gene expressions were elevated in all experimental groups (p=0.008, p=0.001, p=0.022, and p=0.013, respectively). In the heart, a decreased expression of eNOS in group 1 (p=0.04) was observed. Conclusions: Liver cirrhosis leads to histological and histomorphometric alterations in the heart and kidneys, with changes in the NOS and eNOS gene expressions, that may suggest a role in the associated myocardial and renal manifestations.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502021000800204&tlng=enBile Ducts, ExtrahepaticLiver CirrhosisModels, AnimalRats
spellingShingle Ana Cristina Aoun Tannuri
Leiliane Somoggi Chavez
Juliana Xavier Guimarães
Josiane de Oliveira Gonçalves
Suellen Serafini
Gabriela Carvalho de Souza
Denise Maria Avancini Costa Malheiros
Vitor Ribeiro Paes
Uenis Tannuri
Cardiac and renal effects of liver cirrhosis in a growing animal model
Acta Cirúrgica Brasileira
Bile Ducts, Extrahepatic
Liver Cirrhosis
Models, Animal
Rats
title Cardiac and renal effects of liver cirrhosis in a growing animal model
title_full Cardiac and renal effects of liver cirrhosis in a growing animal model
title_fullStr Cardiac and renal effects of liver cirrhosis in a growing animal model
title_full_unstemmed Cardiac and renal effects of liver cirrhosis in a growing animal model
title_short Cardiac and renal effects of liver cirrhosis in a growing animal model
title_sort cardiac and renal effects of liver cirrhosis in a growing animal model
topic Bile Ducts, Extrahepatic
Liver Cirrhosis
Models, Animal
Rats
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502021000800204&tlng=en
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