Cardiac and renal effects of liver cirrhosis in a growing animal model
ABSTRACT Purpose: To assess the biochemical, histological, histomorphometric and molecular effects of biliary duct ligation (BDL) induced liver cirrhosis in the heart and kidneys. Methods: Thirty-two weaning rats (21 days old, 50-70 g) underwent BDL and were divided in four groups (euthanasia afte...
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2021-10-01
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Series: | Acta Cirúrgica Brasileira |
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Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502021000800204&tlng=en |
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author | Ana Cristina Aoun Tannuri Leiliane Somoggi Chavez Juliana Xavier Guimarães Josiane de Oliveira Gonçalves Suellen Serafini Gabriela Carvalho de Souza Denise Maria Avancini Costa Malheiros Vitor Ribeiro Paes Uenis Tannuri |
author_facet | Ana Cristina Aoun Tannuri Leiliane Somoggi Chavez Juliana Xavier Guimarães Josiane de Oliveira Gonçalves Suellen Serafini Gabriela Carvalho de Souza Denise Maria Avancini Costa Malheiros Vitor Ribeiro Paes Uenis Tannuri |
author_sort | Ana Cristina Aoun Tannuri |
collection | DOAJ |
description | ABSTRACT Purpose: To assess the biochemical, histological, histomorphometric and molecular effects of biliary duct ligation (BDL) induced liver cirrhosis in the heart and kidneys. Methods: Thirty-two weaning rats (21 days old, 50-70 g) underwent BDL and were divided in four groups (euthanasia after two, four, six, and eight weeks, respectively) and compared to control groups. Results: The animals’ hearts of group 3 were bigger than those of the control group (p=0.042), including thinner right ventricle wall, decreased internal diameter of ventricles, and increased perivascular collagen deposition in left ventricle, as well as increased interstitial collagen in right ventricle after six weeks. In the kidneys of groups 3 and 4, bilirubin impregnation in the tubules, hydropic degeneration, loss of nuclei and lack of plasmatic membrane limits were noted. Nitric oxide synthase (NOS) gene expressions were higher in group 1 (p=0.008), and endothelial nitric oxide synthase (eNOS) gene expressions were elevated in all experimental groups (p=0.008, p=0.001, p=0.022, and p=0.013, respectively). In the heart, a decreased expression of eNOS in group 1 (p=0.04) was observed. Conclusions: Liver cirrhosis leads to histological and histomorphometric alterations in the heart and kidneys, with changes in the NOS and eNOS gene expressions, that may suggest a role in the associated myocardial and renal manifestations. |
first_indexed | 2024-12-22T02:29:04Z |
format | Article |
id | doaj.art-df0ffd1941a44140a103482663f9895d |
institution | Directory Open Access Journal |
issn | 0102-8650 |
language | English |
last_indexed | 2024-12-22T02:29:04Z |
publishDate | 2021-10-01 |
publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
record_format | Article |
series | Acta Cirúrgica Brasileira |
spelling | doaj.art-df0ffd1941a44140a103482663f9895d2022-12-21T18:41:55ZengSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira0102-86502021-10-0136810.1590/acb360806Cardiac and renal effects of liver cirrhosis in a growing animal modelAna Cristina Aoun Tannurihttps://orcid.org/0000-0002-5481-032XLeiliane Somoggi Chavezhttps://orcid.org/0000-0003-2577-3598Juliana Xavier Guimarãeshttps://orcid.org/0000-0001-9434-6472Josiane de Oliveira Gonçalveshttps://orcid.org/0000-0002-5402-6455Suellen Serafinihttps://orcid.org/0000-0001-5853-5743Gabriela Carvalho de Souzahttps://orcid.org/0000-0003-1155-7464Denise Maria Avancini Costa Malheiroshttps://orcid.org/0000-0002-0409-816XVitor Ribeiro Paeshttps://orcid.org/0000-0001-8752-5624Uenis Tannurihttps://orcid.org/0000-0002-3855-3298ABSTRACT Purpose: To assess the biochemical, histological, histomorphometric and molecular effects of biliary duct ligation (BDL) induced liver cirrhosis in the heart and kidneys. Methods: Thirty-two weaning rats (21 days old, 50-70 g) underwent BDL and were divided in four groups (euthanasia after two, four, six, and eight weeks, respectively) and compared to control groups. Results: The animals’ hearts of group 3 were bigger than those of the control group (p=0.042), including thinner right ventricle wall, decreased internal diameter of ventricles, and increased perivascular collagen deposition in left ventricle, as well as increased interstitial collagen in right ventricle after six weeks. In the kidneys of groups 3 and 4, bilirubin impregnation in the tubules, hydropic degeneration, loss of nuclei and lack of plasmatic membrane limits were noted. Nitric oxide synthase (NOS) gene expressions were higher in group 1 (p=0.008), and endothelial nitric oxide synthase (eNOS) gene expressions were elevated in all experimental groups (p=0.008, p=0.001, p=0.022, and p=0.013, respectively). In the heart, a decreased expression of eNOS in group 1 (p=0.04) was observed. Conclusions: Liver cirrhosis leads to histological and histomorphometric alterations in the heart and kidneys, with changes in the NOS and eNOS gene expressions, that may suggest a role in the associated myocardial and renal manifestations.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502021000800204&tlng=enBile Ducts, ExtrahepaticLiver CirrhosisModels, AnimalRats |
spellingShingle | Ana Cristina Aoun Tannuri Leiliane Somoggi Chavez Juliana Xavier Guimarães Josiane de Oliveira Gonçalves Suellen Serafini Gabriela Carvalho de Souza Denise Maria Avancini Costa Malheiros Vitor Ribeiro Paes Uenis Tannuri Cardiac and renal effects of liver cirrhosis in a growing animal model Acta Cirúrgica Brasileira Bile Ducts, Extrahepatic Liver Cirrhosis Models, Animal Rats |
title | Cardiac and renal effects of liver cirrhosis in a growing animal model |
title_full | Cardiac and renal effects of liver cirrhosis in a growing animal model |
title_fullStr | Cardiac and renal effects of liver cirrhosis in a growing animal model |
title_full_unstemmed | Cardiac and renal effects of liver cirrhosis in a growing animal model |
title_short | Cardiac and renal effects of liver cirrhosis in a growing animal model |
title_sort | cardiac and renal effects of liver cirrhosis in a growing animal model |
topic | Bile Ducts, Extrahepatic Liver Cirrhosis Models, Animal Rats |
url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502021000800204&tlng=en |
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