Structure elucidation, immunomodulatory activity, antitumor activity and its molecular mechanism of a novel polysaccharide from Boletus reticulatus Schaeff
A new water-soluble heteropolysaccharide with a molecular weight of 15 kDa was isolated from the fruiting bodies of Boletus reticulatus Schaeff. Structural characterization results revealed that B. reticulatus Schaeff polysaccharide (BRS-X) had a backbone of 1,6-linked α-D-galactose and 1,2,6-linked...
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Tsinghua University Press
2023-03-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022001793 |
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| author | Siyuan Su Xiang Ding Yiling Hou Binbin Liu Zhouhe Du Junfeng Liu |
| author_facet | Siyuan Su Xiang Ding Yiling Hou Binbin Liu Zhouhe Du Junfeng Liu |
| author_sort | Siyuan Su |
| collection | DOAJ |
| description | A new water-soluble heteropolysaccharide with a molecular weight of 15 kDa was isolated from the fruiting bodies of Boletus reticulatus Schaeff. Structural characterization results revealed that B. reticulatus Schaeff polysaccharide (BRS-X) had a backbone of 1,6-linked α-D-galactose and 1,2,6-linked α-D-galactose which branches were mainly composed of a terminal 4-linked β-D-glucose and the ratio of D-galactose and D-glucose was 5:1. Bioactivity assays indicated that BRS-X displayed a strong proliferative activity in T cells and B cells and promoted the secretion of immunoglobulin G (IgG), IgE, IgD and IgM. In addition, BRS-X could facilitate the proliferation and phagocytosis of RAW264.7 cells and could significantly inhibit the growth of tumors in S180-bearing mice. The results of transcriptome sequencing analysis illustrated that total 46 genes enriched in MAPK and total 34 genes enriched in PI3K/Akt signaling pathways in BRS-X group. The protein VEGF and VEGFR expression were significantly reduced under the treatment with BRS-X. These findings provide a scientific basis for the edible and medicinal value of BRS-X. |
| first_indexed | 2024-03-12T11:01:54Z |
| format | Article |
| id | doaj.art-df11b455570748d991f756fb84128034 |
| institution | Directory Open Access Journal |
| issn | 2213-4530 |
| language | English |
| last_indexed | 2024-03-12T11:01:54Z |
| publishDate | 2023-03-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj.art-df11b455570748d991f756fb841280342023-09-02T05:21:34ZengTsinghua University PressFood Science and Human Wellness2213-45302023-03-01122647661Structure elucidation, immunomodulatory activity, antitumor activity and its molecular mechanism of a novel polysaccharide from Boletus reticulatus SchaeffSiyuan Su0Xiang Ding1Yiling Hou2Binbin Liu3Zhouhe Du4Junfeng Liu5Key Laboratory of Southwest China Wildlife Resources Conservation, College of Life Sciences, China West Normal University, Nanchong 637009, China; Sericultural Research Institute, Sichuan Academy of Agricultural Sciences, Nanchong 637000, ChinaCollege of Environmental Science and Engineering, China West Normal University, Nanchong 637009, ChinaKey Laboratory of Southwest China Wildlife Resources Conservation, College of Life Sciences, China West Normal University, Nanchong 637009, China; Corresponding author at: Key Laboratory of Southwest China Wildlife Resources Conservation, College of Life Sciences, China West Normal University, Nanchong 637009, China.Sericultural Research Institute, Sichuan Academy of Agricultural Sciences, Nanchong 637000, ChinaSericultural Research Institute, Sichuan Academy of Agricultural Sciences, Nanchong 637000, ChinaSericultural Research Institute, Sichuan Academy of Agricultural Sciences, Nanchong 637000, ChinaA new water-soluble heteropolysaccharide with a molecular weight of 15 kDa was isolated from the fruiting bodies of Boletus reticulatus Schaeff. Structural characterization results revealed that B. reticulatus Schaeff polysaccharide (BRS-X) had a backbone of 1,6-linked α-D-galactose and 1,2,6-linked α-D-galactose which branches were mainly composed of a terminal 4-linked β-D-glucose and the ratio of D-galactose and D-glucose was 5:1. Bioactivity assays indicated that BRS-X displayed a strong proliferative activity in T cells and B cells and promoted the secretion of immunoglobulin G (IgG), IgE, IgD and IgM. In addition, BRS-X could facilitate the proliferation and phagocytosis of RAW264.7 cells and could significantly inhibit the growth of tumors in S180-bearing mice. The results of transcriptome sequencing analysis illustrated that total 46 genes enriched in MAPK and total 34 genes enriched in PI3K/Akt signaling pathways in BRS-X group. The protein VEGF and VEGFR expression were significantly reduced under the treatment with BRS-X. These findings provide a scientific basis for the edible and medicinal value of BRS-X.http://www.sciencedirect.com/science/article/pii/S2213453022001793Boletus reticulatus SchaeffPolysaccharidesStructure identificationImmunomodulatory activityAntitumor activity |
| spellingShingle | Siyuan Su Xiang Ding Yiling Hou Binbin Liu Zhouhe Du Junfeng Liu Structure elucidation, immunomodulatory activity, antitumor activity and its molecular mechanism of a novel polysaccharide from Boletus reticulatus Schaeff Food Science and Human Wellness Boletus reticulatus Schaeff Polysaccharides Structure identification Immunomodulatory activity Antitumor activity |
| title | Structure elucidation, immunomodulatory activity, antitumor activity and its molecular mechanism of a novel polysaccharide from Boletus reticulatus Schaeff |
| title_full | Structure elucidation, immunomodulatory activity, antitumor activity and its molecular mechanism of a novel polysaccharide from Boletus reticulatus Schaeff |
| title_fullStr | Structure elucidation, immunomodulatory activity, antitumor activity and its molecular mechanism of a novel polysaccharide from Boletus reticulatus Schaeff |
| title_full_unstemmed | Structure elucidation, immunomodulatory activity, antitumor activity and its molecular mechanism of a novel polysaccharide from Boletus reticulatus Schaeff |
| title_short | Structure elucidation, immunomodulatory activity, antitumor activity and its molecular mechanism of a novel polysaccharide from Boletus reticulatus Schaeff |
| title_sort | structure elucidation immunomodulatory activity antitumor activity and its molecular mechanism of a novel polysaccharide from boletus reticulatus schaeff |
| topic | Boletus reticulatus Schaeff Polysaccharides Structure identification Immunomodulatory activity Antitumor activity |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022001793 |
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