Utilization of marigold (Tagetes erecta) flower fermentation wastewater as a fertilizer and its effect on microbial community structure in maize rhizosphere and non-rhizosphere soil
Marigold (Tagetes erecta L.) flowers can be used to produce lutein, but the production process generates fermentation wastewater, which is rich in lactic acid. Currently, most fermentation wastewater treatment methods are relatively expensive and time-consuming. To develop a practical marigold flowe...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2020-01-01
|
| Series: | Biotechnology & Biotechnological Equipment |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/13102818.2020.1781548 |
| _version_ | 1818920761345179648 |
|---|---|
| author | Hongwei Liu Yana Wang Cong Liang Qingxia Yang Shuai Wang Buqing Wang Feiyan Zhang Liping Zhang Huicai Cheng Shuishan Song Liping Zhang |
| author_facet | Hongwei Liu Yana Wang Cong Liang Qingxia Yang Shuai Wang Buqing Wang Feiyan Zhang Liping Zhang Huicai Cheng Shuishan Song Liping Zhang |
| author_sort | Hongwei Liu |
| collection | DOAJ |
| description | Marigold (Tagetes erecta L.) flowers can be used to produce lutein, but the production process generates fermentation wastewater, which is rich in lactic acid. Currently, most fermentation wastewater treatment methods are relatively expensive and time-consuming. To develop a practical marigold flower fermentation wastewater treatment method, in this study, we treated the fermentation wastewater with calcium oxide to prepare a neutralization solution, which was then used to irrigate maize that in turn exhibited a significant increase in growth. Then, we investigated soil pH, conductivity and enzyme activity, as well as microbial community and diversity of the maize rhizosphere and non-rhizosphere soil. Compared with the control group, the neutralization solution had no significant effect on soil pH and four kinds of enzyme (soil urease, catalase, sucrase and acid phosphatase) activity, but the electrical conductivity of the treatment group increased by 24.3%. The 16s rDNA and ITS rDNA sequencing results showed that the neutralization solution treatment influenced the diversity and abundance of bacteria and fungi in the non-rhizosphere samples, as well as the number of bacterial species in rhizosphere soil. The effect of neutralization solution treatment on bacteria was greater than that on fungi. Proteobacteria and Ascomycota were the predominant bacterial and fungal phyla in maize rhizosphere soil. The relative abundance of Paenibacillus, Bacillus and Penicillium genera significantly increased in non-rhizosphere soils. We hereby present a potential method for treating marigold flower fermentation wastewater for fertilizer utilization and show that the neutralization solution promotes maize growth and influences the soil microbial structure. |
| first_indexed | 2024-12-20T01:26:53Z |
| format | Article |
| id | doaj.art-df154360d5264504a526ec84c10d90e3 |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-12-20T01:26:53Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-df154360d5264504a526ec84c10d90e32022-12-21T19:58:13ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302020-01-0134152253110.1080/13102818.2020.17815481781548Utilization of marigold (Tagetes erecta) flower fermentation wastewater as a fertilizer and its effect on microbial community structure in maize rhizosphere and non-rhizosphere soilHongwei Liu0Yana Wang1Cong Liang2Qingxia Yang3Shuai Wang4Buqing Wang5Feiyan Zhang6Liping Zhang7Huicai Cheng8Shuishan Song9Liping Zhang10Department of Microbiology, College of Life Science, Hebei UniversityMicrobiology Laboratory, Institute of Biology, Hebei Academy of ScienceMicrobiology Laboratory, Institute of Biology, Hebei Academy of ScienceMicrobiology Laboratory, Institute of Biology, Hebei Academy of ScienceDepartment of Biopharmaceuticals, College of Pharmacy, Liaocheng UniversityMicrobiology Laboratory, Institute of Biology, Hebei Academy of ScienceMicrobiology Laboratory, Institute of Biology, Hebei Academy of ScienceMicrobiology Laboratory, Institute of Biology, Hebei Academy of ScienceMicrobiology Laboratory, Institute of Biology, Hebei Academy of ScienceMicrobiology Laboratory, Institute of Biology, Hebei Academy of ScienceDepartment of Microbiology, College of Life Science, Hebei UniversityMarigold (Tagetes erecta L.) flowers can be used to produce lutein, but the production process generates fermentation wastewater, which is rich in lactic acid. Currently, most fermentation wastewater treatment methods are relatively expensive and time-consuming. To develop a practical marigold flower fermentation wastewater treatment method, in this study, we treated the fermentation wastewater with calcium oxide to prepare a neutralization solution, which was then used to irrigate maize that in turn exhibited a significant increase in growth. Then, we investigated soil pH, conductivity and enzyme activity, as well as microbial community and diversity of the maize rhizosphere and non-rhizosphere soil. Compared with the control group, the neutralization solution had no significant effect on soil pH and four kinds of enzyme (soil urease, catalase, sucrase and acid phosphatase) activity, but the electrical conductivity of the treatment group increased by 24.3%. The 16s rDNA and ITS rDNA sequencing results showed that the neutralization solution treatment influenced the diversity and abundance of bacteria and fungi in the non-rhizosphere samples, as well as the number of bacterial species in rhizosphere soil. The effect of neutralization solution treatment on bacteria was greater than that on fungi. Proteobacteria and Ascomycota were the predominant bacterial and fungal phyla in maize rhizosphere soil. The relative abundance of Paenibacillus, Bacillus and Penicillium genera significantly increased in non-rhizosphere soils. We hereby present a potential method for treating marigold flower fermentation wastewater for fertilizer utilization and show that the neutralization solution promotes maize growth and influences the soil microbial structure.http://dx.doi.org/10.1080/13102818.2020.1781548marigold flower fermentation wastewatercalcium oxide treatmentmicrobial community structuremaize rhizosphere and non-rhizosphere soilplant growth promotionfertilizer |
| spellingShingle | Hongwei Liu Yana Wang Cong Liang Qingxia Yang Shuai Wang Buqing Wang Feiyan Zhang Liping Zhang Huicai Cheng Shuishan Song Liping Zhang Utilization of marigold (Tagetes erecta) flower fermentation wastewater as a fertilizer and its effect on microbial community structure in maize rhizosphere and non-rhizosphere soil Biotechnology & Biotechnological Equipment marigold flower fermentation wastewater calcium oxide treatment microbial community structure maize rhizosphere and non-rhizosphere soil plant growth promotion fertilizer |
| title | Utilization of marigold (Tagetes erecta) flower fermentation wastewater as a fertilizer and its effect on microbial community structure in maize rhizosphere and non-rhizosphere soil |
| title_full | Utilization of marigold (Tagetes erecta) flower fermentation wastewater as a fertilizer and its effect on microbial community structure in maize rhizosphere and non-rhizosphere soil |
| title_fullStr | Utilization of marigold (Tagetes erecta) flower fermentation wastewater as a fertilizer and its effect on microbial community structure in maize rhizosphere and non-rhizosphere soil |
| title_full_unstemmed | Utilization of marigold (Tagetes erecta) flower fermentation wastewater as a fertilizer and its effect on microbial community structure in maize rhizosphere and non-rhizosphere soil |
| title_short | Utilization of marigold (Tagetes erecta) flower fermentation wastewater as a fertilizer and its effect on microbial community structure in maize rhizosphere and non-rhizosphere soil |
| title_sort | utilization of marigold tagetes erecta flower fermentation wastewater as a fertilizer and its effect on microbial community structure in maize rhizosphere and non rhizosphere soil |
| topic | marigold flower fermentation wastewater calcium oxide treatment microbial community structure maize rhizosphere and non-rhizosphere soil plant growth promotion fertilizer |
| url | http://dx.doi.org/10.1080/13102818.2020.1781548 |
| work_keys_str_mv | AT hongweiliu utilizationofmarigoldtageteserectaflowerfermentationwastewaterasafertilizeranditseffectonmicrobialcommunitystructureinmaizerhizosphereandnonrhizospheresoil AT yanawang utilizationofmarigoldtageteserectaflowerfermentationwastewaterasafertilizeranditseffectonmicrobialcommunitystructureinmaizerhizosphereandnonrhizospheresoil AT congliang utilizationofmarigoldtageteserectaflowerfermentationwastewaterasafertilizeranditseffectonmicrobialcommunitystructureinmaizerhizosphereandnonrhizospheresoil AT qingxiayang utilizationofmarigoldtageteserectaflowerfermentationwastewaterasafertilizeranditseffectonmicrobialcommunitystructureinmaizerhizosphereandnonrhizospheresoil AT shuaiwang utilizationofmarigoldtageteserectaflowerfermentationwastewaterasafertilizeranditseffectonmicrobialcommunitystructureinmaizerhizosphereandnonrhizospheresoil AT buqingwang utilizationofmarigoldtageteserectaflowerfermentationwastewaterasafertilizeranditseffectonmicrobialcommunitystructureinmaizerhizosphereandnonrhizospheresoil AT feiyanzhang utilizationofmarigoldtageteserectaflowerfermentationwastewaterasafertilizeranditseffectonmicrobialcommunitystructureinmaizerhizosphereandnonrhizospheresoil AT lipingzhang utilizationofmarigoldtageteserectaflowerfermentationwastewaterasafertilizeranditseffectonmicrobialcommunitystructureinmaizerhizosphereandnonrhizospheresoil AT huicaicheng utilizationofmarigoldtageteserectaflowerfermentationwastewaterasafertilizeranditseffectonmicrobialcommunitystructureinmaizerhizosphereandnonrhizospheresoil AT shuishansong utilizationofmarigoldtageteserectaflowerfermentationwastewaterasafertilizeranditseffectonmicrobialcommunitystructureinmaizerhizosphereandnonrhizospheresoil AT lipingzhang utilizationofmarigoldtageteserectaflowerfermentationwastewaterasafertilizeranditseffectonmicrobialcommunitystructureinmaizerhizosphereandnonrhizospheresoil |