Biological Impact of Phenolic Compounds from Coffee on Colorectal Cancer
Colorectal cancer is one of the leading death-related diseases worldwide, usually induced by a multifactorial and complex process, including genetic and epigenetic abnormalities and the impact of diet and lifestyle. In the present study, we evaluated the biological impact of two of the main coffee p...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-08-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/14/8/761 |
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| author | Hernán Villota Manuel Moreno-Ceballos Gloria A. Santa-González Diego Uribe Isabel Cristina Henao Castañeda Lina María Preciado Johanna Pedroza-Díaz |
| author_facet | Hernán Villota Manuel Moreno-Ceballos Gloria A. Santa-González Diego Uribe Isabel Cristina Henao Castañeda Lina María Preciado Johanna Pedroza-Díaz |
| author_sort | Hernán Villota |
| collection | DOAJ |
| description | Colorectal cancer is one of the leading death-related diseases worldwide, usually induced by a multifactorial and complex process, including genetic and epigenetic abnormalities and the impact of diet and lifestyle. In the present study, we evaluated the biological impact of two of the main coffee polyphenols, chlorogenic acid (CGA) and caffeic acid (CA), as well as two polyphenol-rich coffee extracts (green coffee extract and toasted coffee Extract) against SW480 and SW620 colorectal cancer cells. First, the total phenolic content and the antioxidant capability of the extracts were determined. Then, cytotoxicity was evaluated by MTT and SBR. Finally, a wound healing assay was performed to determine the impact on the cell migration process. The results showed a cytotoxic effect of all treatments in a time and dose-dependent manner, which decreased the viability in both cell lines at 24 h and 48 h; likewise, the migration capability of cells decreased with low doses of treatments. These results suggest the potential of coffee to modulate biological mechanisms involved in colorectal cancer development; however, more studies are required to understand the mechanistic insights of these observations. |
| first_indexed | 2024-03-10T08:29:42Z |
| format | Article |
| id | doaj.art-df15c537a1c5429cbee0ee42b932a5ba |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-10T08:29:42Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-df15c537a1c5429cbee0ee42b932a5ba2023-11-22T09:11:25ZengMDPI AGPharmaceuticals1424-82472021-08-0114876110.3390/ph14080761Biological Impact of Phenolic Compounds from Coffee on Colorectal CancerHernán Villota0Manuel Moreno-Ceballos1Gloria A. Santa-González2Diego Uribe3Isabel Cristina Henao Castañeda4Lina María Preciado5Johanna Pedroza-Díaz6Biomedical Innovation and Research Group, Instituto Tecnologico Metropolitano, Faculty of Applied and Exact Sciences, Medellin 050012, ColombiaBiomedical Innovation and Research Group, Instituto Tecnologico Metropolitano, Faculty of Applied and Exact Sciences, Medellin 050012, ColombiaBiomedical Innovation and Research Group, Instituto Tecnologico Metropolitano, Faculty of Applied and Exact Sciences, Medellin 050012, ColombiaBiomedical Innovation and Research Group, Instituto Tecnologico Metropolitano, Faculty of Applied and Exact Sciences, Medellin 050012, ColombiaProductos Naturales Marinos, Facultad de Ciencias Farmacéuticas y Alimentarias, Universidad de Antioquia UdeA, Medellín 050010, ColombiaToxinología y Alternativas Terapéuticas y Alimentarias, Facultad de Ciencias Farmacéuticas y Alimentarias, Universidad de Antioquia UdeA, Medellín 050010, ColombiaBiomedical Innovation and Research Group, Instituto Tecnologico Metropolitano, Faculty of Applied and Exact Sciences, Medellin 050012, ColombiaColorectal cancer is one of the leading death-related diseases worldwide, usually induced by a multifactorial and complex process, including genetic and epigenetic abnormalities and the impact of diet and lifestyle. In the present study, we evaluated the biological impact of two of the main coffee polyphenols, chlorogenic acid (CGA) and caffeic acid (CA), as well as two polyphenol-rich coffee extracts (green coffee extract and toasted coffee Extract) against SW480 and SW620 colorectal cancer cells. First, the total phenolic content and the antioxidant capability of the extracts were determined. Then, cytotoxicity was evaluated by MTT and SBR. Finally, a wound healing assay was performed to determine the impact on the cell migration process. The results showed a cytotoxic effect of all treatments in a time and dose-dependent manner, which decreased the viability in both cell lines at 24 h and 48 h; likewise, the migration capability of cells decreased with low doses of treatments. These results suggest the potential of coffee to modulate biological mechanisms involved in colorectal cancer development; however, more studies are required to understand the mechanistic insights of these observations.https://www.mdpi.com/1424-8247/14/8/761natural compoundsphenolic compoundscell biologypharmaceutical sciencecoffee |
| spellingShingle | Hernán Villota Manuel Moreno-Ceballos Gloria A. Santa-González Diego Uribe Isabel Cristina Henao Castañeda Lina María Preciado Johanna Pedroza-Díaz Biological Impact of Phenolic Compounds from Coffee on Colorectal Cancer Pharmaceuticals natural compounds phenolic compounds cell biology pharmaceutical science coffee |
| title | Biological Impact of Phenolic Compounds from Coffee on Colorectal Cancer |
| title_full | Biological Impact of Phenolic Compounds from Coffee on Colorectal Cancer |
| title_fullStr | Biological Impact of Phenolic Compounds from Coffee on Colorectal Cancer |
| title_full_unstemmed | Biological Impact of Phenolic Compounds from Coffee on Colorectal Cancer |
| title_short | Biological Impact of Phenolic Compounds from Coffee on Colorectal Cancer |
| title_sort | biological impact of phenolic compounds from coffee on colorectal cancer |
| topic | natural compounds phenolic compounds cell biology pharmaceutical science coffee |
| url | https://www.mdpi.com/1424-8247/14/8/761 |
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